...type- and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...

...if you’re working with plants, don’t be afraid to try your hand at CRISPR. References Belhaj K, Chaparro-Garcia...

...Nelson et al., and Tabebordbar et al. each chose to try exon skipping in DMD mice, which have a mutation ...

...expression in a variety of cell types, but you should try to find the best AAV for your particular experiment...: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15 (2005): 9933...

...collaboration with Prof. Alexis Komor and together they are trying to broaden our understanding of the base editing...

...spread from the injection site it is advisable to try a few different injection titers and volumes to determine...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol 92. https:/...

...tissue tropism for mice in vivo. To target... Try using... Citation CNS AAV1, AAV2, AAV5, AAV8, AAV9... for gene transfer. The Journal of Biological Chemistry, 276(23), 20610–20616. https://doi.org/10.1074...

...or if no monomers exist in your sample, you could try linearizing the plasmid via restriction digestion...colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...

...student at Johns Hopkins University, where he is trying turn a few exoplanets into a successful PhD thesis...ensued as “alternative facts” became a part of our country’s rhetoric and drastic cuts to the NIH budget were...

...enough to go up a kit size, it may be tempting to try increasing your input to increase your output. But...quantity and quality of your plasmid prep is by spectrophotometry. Achieving the highest-quality DNA requires...than half 70% recycled materials Sustainable forestry certification Starch-based compostable bags for...

...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the...its substrate. Then, all it takes is a bit of chemistry magic to produce a wide range of useful molecules...incorporate an alkene to be labeled through click chemistry. Although these multi-component systems can be...brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and HaloTag ligands...Tag for Multiprotein Labeling in Living Cells. Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016...L. (2016, September 8). Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https...https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell...

...Gene does not appear in the auto-populated list, try entering the gene's Official Full Name or one of ...the top right of each data entry page. Once you have completed the data entry process for all of the plasmids...will be saved and the page will open a new set of entry fields to describe the next insert. Repeat as needed...the "Submit Deposit" button to complete the data entry. Clicking the "Submit Deposit" button will allow...

...available to industry, in addition to academics and nonprofits, and this is denoted in the Industry column ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing...this novel virus. It is now known that SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on ...plasmids that have been used for measuring viral entry and activity in neutralization assays and links ...growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV...-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV ... as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find plasmids related...

...tag insertion, you should test a new crRNA and/or try out an alternative tag (3xFLAG, Myc, V5, or HA). ...pull-down assays include immunoprecipitation mass spectrometry (IP/MS) and chromatin immunoprecipitation (ChIP... protein complexes are then analysed by mass spectrometry to identify the interacting proteins. Similarly...analyzed for tagging efficiency using standard immunocytochemistry (ICC) assays.  Figure 1: Three ingredients...single-stranded donor DNA (V5 tag) were quantified by immunocytochemistry assays. Dewari et al, eLife 2018;7:e35069...

...Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry Blues Plasmid...Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry Greens Plasmid...Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description Industry Do you...

...disease-inducing genetic mutation in a patient, trying to transdifferentiate any of the patient’s cells...

...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian...with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko 139547...Cre T. brucei Cross 33345 pLEW100cre-EP1 Cre Trypanosoma brucei Cross 34564 pMSCVpuro-Cre Cre 5'LTR Retroviral...components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish Eisenhoffer 131783 pTol2...139547 pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin Plant...

...Methanogenic archaeon 4-Fluoro-L-Tryptophan (4FTrp), 5-Fluoro-L-Tryptophan (5FTrp) Bacterial TAG Thomas Huber...Methanogenic archaeon 6-Fluoro-L-Tryptophan (6FTrp), 7-Fluoro-L-Tryptophan (7FTrp) Bacterial TAG Thomas Huber...pRSF-G1(7FTrp)RS PylRS M. archaeon 7-Fluoro-L-Tryptophan (7FTrp) Bacterial TAG Thomas Huber 177311 pRSF-G1TFAKRS...7AW)RS G1(7AW)RS Methanogenic archaeon 7-Aza-L-Tryptophan (7AW) Bacterial TAG Thomas Huber 207620 pRSF-...

...Jul 29;7:12266. David Savage Tryptophan Visualize intracellular tryptophan levels and flux Nanosensor detection...green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green- and...characterization of dedicated sensors for FLIM, for ratiometry and with high affinity. PLoS One. 2015 Apr 14... Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. Martin Webb Phosphate... detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10)... Grx1-roCherry. Redox Biol. 2019 Feb;21:101071. Dmitry Bilan Temperature Genetically encoded ratiometric...

...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...kinase-library reactions (SERIOHL-KILR, Barber et al, Biochemistry 2018). Iterative Synthetically Phosphorylated..., Jun JW, Lou HJ, Turk BE, Rinehart J. 2018. Biochemistry . 2018 Aug 7;57(31):4717-4725. PubMed (Link ...