...box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...support the community in developing better, more sustainable, easily sharable products. Open-Source Microscopy...popular plugin-based image process framework FIJI are just three of the constantly growing number of "community-driven...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic...

...Addgenie expertise we can. We even have a Slack channel just for asking people if they can write or review things...touches We try to use figures and images to help illustrate our points and break up walls of text. Since ...designer upon request that the science communicator "just sketch a western blot out quickly." Final design...resources that answer questions our depositors and customers are asking. So how can you get involved? Keep ...

... statistically significant results that are not robust. Sampling noise There is, of course, simple noise...variable actually makes a big difference for the robustness of the findings.   How do we make research more...report the results of all four of those tests and not just the subset of those that return a particular result...not left in the uncomfortable position of having robust, null findings that are notoriously difficult to...

... of material to sift through. Fortunately, we’ve just made it easier for you to find what you need quickly...assembled a cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist...particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...of our information in order to more granularly customize the search engine, and a scoping mechanism that...

... shared in total? Just over 49,000 times! Their long-lasting popularity illustrates the utility these ...S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance.... It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with cell lines ...

...It’s not just the antibodies that matter when you’re prepping for your experiment - there are a number...antibody storage, buffer considerations, and give just the lightest of nods towards conjugates.  Storage...your antibody.  At some point in your long and illustrious lab career, you will likely accidentally leave...signaling molecules! Please keep in mind that this is just a brief introduction to the wide, wild, and fascinating...

... “Learning is not attained by chance, it must be sought for with ardor and attended to with diligence...feel that you are an expert networker (or if you just aren’t doing it at all yet) watch my webinar, “Not... that comes highly recommended from someone you trust. A good coach can make a big difference, if you ...ask someone to share their wisdom and they might just not have the time. Thank them anyway and move on...

...Melina Fan, a cell biology graduate student, was frustrated by the difficulty of accessing published plasmids...special thanks to the early-adopter scientists, who trusted Addgene before we were a “labhold” name. Our first... of all the depositors whose plasmids we were entrusted with. So in June 2016, we launched the Blue Flame...award because you don’t get nominated for it, it just reflects how useful the reagents we created are ...

... composed of just the Fab portion of the antibody. Fab fragments are useful when you just need an antibody...isotype for another. But it also allows you to use just a fragment of the antibody in isolation from another...Size: ~50 kDa. Fv - These fragments are composed of just the variable domains of the heavy and light chains...

...: IL-36 biology in generalized pustular psoriasis Generalized pustular psoriasis is a chronic autoinflammatory...affect 0.27 to 4.6 per 10,000 people globally. Pustules form from the uncontrolled activation of the interleukin...at University of Texas Health Science Center at Houston. Prakash became interested in studying TS when ...

...to discuss their visions for a successful and sustainable biomedical research sector at our first Future... ‘standard’ pathway into academia may in fact be just another ‘alternative’ career.   The news is not ... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates...

...advances from exacerbating existing inequalities and injustices. The Personal Genetics Education Project (pgEd...meetings of experts and leaders from academia, industry, community, education and government; advising...marginalized communities have created a legacy of distrust between these communities and the biomedical establishment...academic venues such as scientific, educational or industry conferences, as well as schools, community events...

...biggest benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different...wells plates onto agar q-trays where the samples must be spread much farther apart before plating. With...prep large quantities of DNA because some countries just can’t receive bacteria through the mail due to laws...automated protocol, we can prep two 96 well plates in just under 3 hours or one 96 well plate in about an hour...

...conditions. As mentioned above, plasmid ratios can be adjusted to enhance titer. In addition the transfection...area of the larger vessel to the 10 cm2 dish and adjusting the cell number, plasmid concentration, and experimental...accordingly. While you may need to make some final adjustments, this is generally a good place to start. If ...will precipitate all proteins in a solution, not just the lentivirus; consequently, this may not be the...

...every meeting Supervisors are busy and your work is just one of the hundred things on their minds. When you.... Avoid talking about your emotions, such as frustration or anger, because your discussion will get side-tracked...supervisor know as soon as possible. In order to build trust between you and your supervisor, you need to demonstrate... Whether your supervisor is a negative person or just an inexperienced manager, you can resolve most conflicts...

... is best for you — and the mice. While not an exhaustive list, these can include what’s being injected...DNA, viral vectors, and viruses. All researchers must go through the proper basic training prior to conducting... a bit of extra prep, as the injection site area must be shaved first. Restraint: anesthesia Location:...injections are used when the substance being administered must be delivered to the central nervous system via cerebral...

...compensation values or adjust the values with up/down arrow buttons. For anyone just starting to use flow...compensation means you are telling your cytometer to adjust the fluorescent signals by a specific value between...samples. This means that, unlike voltages, you can adjust compensation values later in your analysis, unlike...

... community. The rapid adoption of new tools, illustrated in Figure 2, and the longevity of tools seen ...FR=France; KR=South Korea; CH=Switzerland; AU=Australia.  It probably comes as no surprise that the...plasmids. But many other countries, from Japan to Australia, are significant depositors and requestors. CRISPR...per working day, including appropriate MTAs and customs forms. Of course, ordering the plasmids is the ...

... Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new tools for unraveling the multifaceted...molecular biology. And the Myc1-9E10 antibody remains a trusty antibody partner; as I write this, there are close...original mouse IgG1. And the refinement of this trusty tool doesn’t end there: you can also find anti-...fragments.  Abi, on the other hand, might just be the antibody Mary Poppins!   All of the examples...

... al 2016 in a letter to Protein & Cell. Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED...complex with an ssDNA guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an...not be present in AT-rich regions of the genome or just after a stop codon (as required for C-terminus epitope...Take Pooran's NgAgo Survey Survey results As of August 1, 2016, a total of 165 researchers responded to...