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We narrowed to 284 results for: cas9 plasmid

Showing: 141 - 150 of 284 results

  1. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method..., relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish ..., and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these...collection Read our molecular biology reference Find plasmids for your research ...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...(Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile... These two steps are (Figure 1): The initial plasmid integration step (the so-called “single crossover... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...15109831. Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with lambda red recombineering enables simplified...

  3. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...restriction enzyme or PCR to make linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave...Ligation Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost effective...Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage combined with Gibson assembly ... Zhao X, Gabrieli T, Lou C, Ebenstein Y, Zhu TF. Cas9-Assisted Targeting of CHromosome segments CATCH ...Resources on the Addgene Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques ...Techniques Additional Resources on Addgene.org Find Plasmid Cloning Protocols Find Virus Protocols Find Basic...

  4. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...following components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium ...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a...the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to the DNA to induce... of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria links the...encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate Phage infection...Phage-assisted evolution of base-editing activity. A plasmid-based genetic circuit in the bacteria links the...off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors...

  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just ...correctly banded clones for plasmid sequencing and found 98% of the plasmids were perfectly correct. Because... Mammalian Plasmid pRDA_791 (contains only orio and ampR) Drosophilia Plasmid pRDB_051 (for...depending on above selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism...the assembled plasmid. It shows you each component, with the ID of the component plasmid, component name...the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating...resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: Golden Gate Cloning...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy not...Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...Entry DNA overhangs may be present in the original plasmid (Option 1) or added using PCR-based amplification...fragments to create TAL effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type...makes it easy to create a single gRNA-expressing plasmid, but it can also be adapted to express multiple...PubMed. More Resources at Addgene:  Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease ...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...head over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively...nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library is ...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair...linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable ABE.  Find the plasmid here ...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the viral service A fast blue-light...

  9. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors... deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid of 2017 is...envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin...to check out the Trono Lab website. If you have plasmids you’d like to deposit with Addgene, be sure to...Who knows, maybe yours will be the top requested plasmid of 2018! Additional Resources Quick Guide to ...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR...fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study...University of Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal... al. “Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nature...
Showing: 141 - 150 of 284 results