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We narrowed to 728 results for: STO

Showing: 421 - 450 of 728 results

  2. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should be constructed...the GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro ...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...would be useful for their own research, and helps requestors use the plasmid in their own labs. We highly ...survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such as some...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...Addgene's new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our...

  7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...some integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org... Tissue Fixation & the Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    ..., M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating...PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. J., & Wold, W....

  10. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...to late-career grant professionals have similar stories about a time when their passion for an organization...their new career path.  A part of the career path story for most established grant professionals is learning...

  12. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter selection...marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed...

  13. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...64 possible codons but only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for...preferences among organisms aren’t completely understood, but some possible reasons include: Metabolic...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...produced in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus in cell lines...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein ...

  15. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right...and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA ...

  18. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...cloned by two separate labs (Bird et al., 1988; Huston et al., 1988). scFvs are the smallest unit of an.... https://doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny...

  20. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

  21. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...

  22. March for Science

    Type
    Blog Post
    ...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

  24. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...However, ART is not an HIV-1 cure. In patients who stop ART, viral levels will soon skyrocket back to pre-treatment...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription...

  25. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... to the new genomic locus. Image courtesy of Christopher Fell, PhD. After minimizing the system's...enhancer-AAV constructs are now available as in-stock viral preps using the systemic PHP.eB capsid, to...

  26. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Availability of AAV serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1  ✓ ✓ AAV2... Data Hub AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ...

  27. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...

  28. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies...platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol...
Showing: 421 - 450 of 728 results