We narrowed to 844 results for: Tat

...its gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this ..., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its...

...Office of Postdoctoral Affairs at UNC, called her presentation “Disequilibrium, Disillusion and The Postdoc...pretty much said it all.  She cited the oft quoted statistic that only 15% of current postdocs will be academic...Biology at Harvard Medical School, titled her presentation “How to Train Your PI”.   She focused her talk...

...but many throughout the world are affected by devastating diseases that affect far fewer people and therefore...provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi - Spinal muscular...Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and is affiliated with the University of...

...for scientific titles generated from the United States Standard Occupational Classification System  and...they are based on the US Occupational Employment Statistics (OES), but international Twitter users were included...female to male scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists...

...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes... Plasmid sharing in Europe: Addgene’s request statistics Since 2004, Addgene has shipped more than 75,...Years of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of plasmid sharing? ...

... the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2...resulting in a decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio...These plasmids are available as a collection, facilitating screening across the transducerome, to gain ...

...techniques offer researchers different advantages and limitations.  The four main factors in which chemogenetics... The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow neurons to... once can result in several hours of neuronal excitation or inhibition. This sustained activation period...

...wild-type version of YGOI to rescue a mutated strain or a mutated version of YGOI to test the different...different phenotypes of the specific mutations. For these purposes Addgene has a large collection of yeast expression...

...immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. ...fusion protein while it is still bound to the glutathione resin, eliminating the need for an extra clean...

... making plasmid maps, but can also analyze and annotate any DNA sequence. SnapGene Viewer: The free SnapGene...donation-based plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained...analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates...

...balance and were content. As a worker, there are expectations whether or not you work in the lab. My first...capstone projects during the Summer Intern Poster Presentation.  As someone who had limited experience ..., I worked on my capstone project report and presentation, during the final week at Addgene. This report...

...another email notifying me of my acceptance. I was ecstatic! I was going to become a serious scientific wordsmith...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique... D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression in Pseudomonas...

...you communicate your science, whether it’s in presentations, manuscripts, or social media. If you want ...usually ok for informal settings like lab meeting presentations. You can download larger images without the ...means the images are probably better suited for presentations and digital articles where it’s easier to give...

...research is improving lives worldwide and the presentation made it clear how important it is for us to ...say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles! We also...early on Saturday morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the ...

...your messaging and your expectations of your audience, and their expectations of you, consistent and clear... racial/ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed...

...-Beltran et al., 2021 with permission.   Mutations in the spike protein are one of the key drivers...the B.1.351 variant, which has only 9 different mutations compared to the wild-type, escaped neutralization... degree of escape from a very small number of mutations was surprising,” says Balazs.   Figure ...

...negative regulation and can be manipulated with mutations in the replicon. For example, the pMB1 ori maintains...per cell, while pUC – which differs by only two mutations – will produce as many as 700 copies per cell.... numbers since the bacteria have just reached stationary phase, but the cells have not started to die ...

...calls, how about trying your hand at a video presentation? Most video platforms make it easy to record...get their input as well. Giving a great video presentation is going to be a useful skill for the foreseeable... Science: Where to Search for Funding The Ohio State University; Graduate Student Funding Opportunities...

...the Center for Applied Plant Sciences at The Ohio State University. Established in 1991, the Arabidopsis...Arabidopsis and related species. Located at The Ohio State University in Columbus, Ohio, ABRC ships more than...the Center for Applied Plant Sciences at The Ohio State University. In her work, she focuses in public engagement...

...Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on a reduced glutathione matrix...protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic analysis...

... Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in vivo Nathaniel...received by a cell. These so-called recombinase-based state machines could potentially be used to report upon...this post to learn more about recombinase-based state machines and how they function. Adapting Toehold...

...journal will help a Mentee track progress, adjust expectations, and record milestones in reaching goals. The...that I try to exercise every morning, but the temptation to skip the gym is strong. There are a few women...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...

... do with them. In some cases, there might be a mutation that disrupts the function of YFG. You could compare... compare this mutant fly to one lacking the mutation in the same genetic background. In other cases, YFG... knock down gene expression, rescue knock out mutations, and overexpress viral genes. Additionally, other...

...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy ...Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced writing ...including: Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer...

...  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared...possible to do things like in vivo imaging.   Excitation with NIR light also produces less non-specific... non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be ...

...elements often determines the type of plasmid and dictates how it might be used in the lab. Regardless of... Addgene Resource(s) Cloning Plasmids Used to facilitate the cloning of DNA fragments. Cloning vectors... gene function as well as identify changes or mutations that may cause certain diseases. Sequencing DNA...

.... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small...improves editing efficiency. Importantly, the point mutations created by Agudelo et al. prevent ouabain binding...DNA molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the...

...than indirect ones and can be used in highly quantitative assays. On the other hand, they are not as sensitive...stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ...detection. Film also needs to be digitized for quantitative work — and with the advent of online lab notebooks...high-abundance single protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods...

...long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...traditionally have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and...challenge due to multiple factors, including limitations of number of flourescent proteins that can be...