We narrowed to 755 results for: ELP

...Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book....

...to be aware of and actively use tools designed to help reduce or eliminate bias, just like the ones made...

... and of the normal, replicative life cycle — can help you understand these seemingly odd choices and lay...

... R1 institutions to community colleges, and has helped provide original research experiences for thousands...

...about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment out of the park...

...its absorbance is plotted on the y-axis. With the help of graphing software, you then generate a trendline...

... supernatant for further processing. It may be helpful to incorporate regular feeds during this incubation...

... deleted. These genes’ functions are rescued by helper plasmids used during production and/or by genes...

...approaches you with glowing feedback from a client you helped. Maybe you awkwardly say thanks and move on with...

...sequence manager, such as SnapGene, can be very helpful to confirm primer design.  Use minimal template...

...Brett Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke...

...between labs, initial titration experiments might be helpful to achieve optimal results. It is important to ...

...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...

...constructs like plasmids often include GFP as a marker to help identify which cells have successfully taken up ...

...overall size), using TnpB as the core nuclease could help keep the total construct size smaller than Cas9 ...

...highlight sequence identities in problem areas, helping us pinpoint the issue and resolve the assembly....

...AAG to improve editing efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient...

...models, tissue banks, Rett databases, and other helpful tools.  References: Amir RE, Van den Veyver IB...

...such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So when do you want to codon...

...piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker...

...Our internal software was originally designed to help us work in large formats, such as 96-well microplates...

...have any questions, don’t hesitate to email us at help@addgene.org. Happy screening! This blog was written...

...target is not expressed. The negative control will help you determine if there is non-specific binding or...

...overview of the IP method. If you found this blog helpful be sure to check out Addgene’s blog for other antibody...

...serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol...

...created an array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated...

...Shintani et al., 2015). Now, bioinformatics tools help researchers determine how compatible their plasmids...

...offering advice on areas where you might need some help. Today: Career Planning Resources. The key to ensuring...

... brand new location, with more space to grow and help scientists share resources in new ways. Along with...

...analyzed. While the many different ELNs out there can help with searchability, record-keeping, and data sharing...

...necessary or for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies...

...problematic, however. Well designed aptamers can help reduce such background, thereby improving traditional...

...option in such instances is palliative care, which helps patients manage the pain and discomfort caused by...

...bound of activation that current Cas9 systems cannot help you pass. In addition, activation experiments often...

... cells with fresh medium. Daily medium exchanges help alleviate cellular stresses associated with pH changes...

... lab, check out Addgene’s plasmid collections to help start your experiments! References: Blount, Z. ...

...with a versatile, synthetic MCS (designed with the help of the DNA tuner program) (Latynski and Valentovich...

...and CRISPR. Our CRISPR 101 series was designed to help explain the basic principles driving CRISPRs and...

...identifying the cells you are studying. Antibodies can help reveal cellular identities by targeting marker proteins...

...understand their roles in forming the cilia that help establish left-right organization of the embryo ...