We narrowed to 1,054 results for: STI

...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...were unable to deduce the mechanism responsible we still believe that these results will be beneficial to...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...

... have the potential to provide improvements to existing technologies based on polymerase processivity....stable, highly soluble, and non-immunogenic yet are still just as specific to their target as regular antibodies...podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics: developing and applying...analysed. In this work, the authors specifically investigated protein binding at the TERT gene, but this method...

... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual... weird and rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...

... of the primer assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen...and then TAAGCA to improve restriction enzyme digestion. This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA... You can generate the reverse-complement using existing software (a quick internet search will lead you... The PCR product is now ready for restriction digestion. As such, the later steps in this process are ...

...labs. We’re overjoyed to provide you with these fantastic educational resources and will be sure to keep...us at [email protected]. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015...UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous end joining...career coaches do and offers up some career coach suggestions. 8. A Match Made in Heaven: CRISPR/Cas9 and ...

... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect... Steven Farber and colleagues at the Carnegie Institute for Science have developed the LipoGlo system ...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif...displays high dynamic range. However, the promoter in question must be σ54-dependent and the system may not be...

...scientific question that they are eager to discuss with others and sending out these questions ahead of ...Figure 1: Virtual lab meeting? No problem! You can still creatively collaborate and brainstorm! Image from... be written by several authors from different institutes, disciplines, and cultural backgrounds.” This...

...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a colleague... “it was nice meeting you.” Include follow-up questions or requests for advice based on your discussion...LinkedIn I once helped by answering one simple question so I accepted the connection invitation. They ...became closer. She supported me and answered my questions and honestly, she was the only person who really...

...for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice even while working...for bench scientists to assist in labs that are testing for COVID-19. You can also give back by supporting...interview with you, or look for professional societies hosting virtual networking events. Download Addgene’s Science...expectations for your productivity Set a routine and stick to it. Plan defined work hours as well as time for...

...Serendipitous Stain. https://hardydiagnostics.com/wp-content/uploads/2016/05/Hans-Christian-Gram.pdf Smith, A. ...infected lung tissue, a Danish biologist named Christian Gram realized that certain stains were retained...Stain. https://my.clevelandclinic.org/health/diagnostics/22612-gram-stain Hardy, J. Gram’s Serendipitous...

...and white. The decor was very contemporary and futuristic with block chairs in the hallway for people to...what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with a lot of...’m hoping to learn from this workshop? These questions will help me avoid being overwhelmed by all the...instead allow me to pay attention and answer my key questions. As an added bonus, I also got the opportunity...

...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...can submit new fluorescent proteins, add data to existing proteins when missing, or edit inaccuracies as...these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what you’re looking...Plans for future development of FPbase FPbase is still very much in the early stages of development, and...

...just focus on the parts where people had questions or suggestions, instead of reading the whole thing together...gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each...

...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often a selectable...your construct in target cells transiently or constitutively depending on the virus used. All of these ...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...

...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...protocol, from a full page to a few key reminders on a sticky note   Visual Planning  Plate maps, pre-labeling...you need to be successful! Slowing Down At the suggestion of a postdoc, to whom I am forever grateful, ... of things, including (and maybe especially) suggestions you are initially resistant to. Just like any...

...answer to any of these questions is “yes,” then I would suggest either testing the antibody efficacy through...incident to your records, and consider the following questions as you move forward:  Was a light-sensitive conjugate...for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out, however...

...chemically modified to resist degradation while still effectively serving their function of guiding Cas9...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...molecule) you will not have that option, but you can still choose what dye to use  What to do once you’ve chosen...

...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...neuroscience and you don’t have a background in statistics and programming, you’d be wise to start working...surprised at how easily I could jump in and ask questions about the various posters I visited. If you’re...neuroscientist looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene...

...focus of the application was changed, but Sample6 still leverages the core engineering and scientific concepts...communication styles in academia and industry vary drastically, he reassures that one can learn it by reading...into the local academic community. However, Mike still could not remove himself from the idea of starting... many universities throw out old equipment that still works. “We use functional spectrometers from the...

...Mentoring series will cover 2 of the 7 mentoring questions I set out to answer. First, I will describe some...diligent about replying and thinking about good questions and answers. Professional networking groups will...and move on. If they say yes, be prepared with questions and topics to discuss. The mentee is responsible... needs to be open to change and constructive suggestions and feedback. The best thing to do to make your...

...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic...you will need to make sure that you can easily distinguish each different type of antibody and therefore... through the above steps, you may find that you still have a number of options. If this is the case, you...your assays up and running.  Ed.'s note: More questions on antibody validation? Check out Antibodies 101...

... post was written by Professor Mark Howarth, investigator at the University of Cambridge. His research...the features that would define a good tool for sticking together biological components. Nearly all of ...of us played with LEGO as a child (some of us still do), so we are used to the idea of general interfaces...Biology reactions can add new functionality to existing genes, while being accessible simply by dialing...

..., a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted protein degradation In the Bradner and.... Remarkably, small molecule degraders provide distinct advantages over pharmacological inhibitors including...address your requests for dTAG molecules and any questions to Behnam Nabet ([email protected] fellow at the Dana-Farber Cancer Institute. He is a chemical biologist interested in targeted...

...Shaner et al, 2008). This brings us to the key question: what is the best way to compare the photostability...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

...Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful Cas9 toolbox, Ma ... different fluorescent protein to create three distinct colors. The specificity of these orthologs is ...yellow, cyan, or magenta light. A gRNA with all 3 distinct hairpin sequences produces white light. Created...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...

...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...experienced: wanting to ask and answer experimental questions, but not having the tools needed to do so. Cepko... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found...

...many important questions, areas for informed discussion, debate, and data collection still remain: What...workforce structure? Helping to answer some of these questions, and posing some of their own are the following...grassroots organisations that represent postdocs and institutes that employ them, the diversity of trainee backgrounds...

...funding from a variety of government and academic institutions. New graduate students may assume that their...the mission of the application and the funding institution. Moreover, avoidance of ‘to be’ verbs creates... with the description of the RFA and funding institution. Emphasizing how your solution fits into the ...research project grant application" by the NIH Still want more information on the application process...

...and application should be considered The first question you may have is whether a monoclonal or polyclonal... 1998). Consequently, if you find yourself in a sticky species-on-species situation, and other antibody...that users choose antibodies that are easily distinguishable from each other. In a direct approach, that... species or isotype that can be probed with a distinctly conjugated secondary antibody. Consider too that...

...changeover had no impact on our ability to provide requesting scientists high quality data! Addgene's new ... example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...develop a PCR strategy suitable for the library in question. We will order primers that can amplify the insert...

... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of...pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode...

...pattern of the antibody binding “distinct imaged pattern.” The distinct imaged pattern should align with...comparison on the antibody signal’s location. If a distinct imaged pattern does NOT align with the atlases...through their own analysis of the background noise, distinct imaged pattern, and exposure times. To test the...

...plasmids can lead to far reaching consequences, from wasting time and money on optimizing experiments, to false...all incoming plasmids. If you are interested in requesting one of these plasmids, you can request that it...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...team leaves a note on the plasmid page to make requesting scientists aware of the identified discrepancy...

...and overlapping complexes that have distinct functions. Many existing methods for identifying PPIs, such...additional interacting factors. Problems with Existing Methods for Studying Protein-Protein Interactions... biotinylated. This makes it’s impossible to distinguish proteins that directly interact with the bait...

...transduced cells and loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes...virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding an inner core which contains...use of this system. New and improved systems are still being developed. A detailed protocol for production...mL of sample. The purity of the sample can be estimated by looking at the ration of DNA (260nm) versus...

...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin...box for the “Purpose” field. If you ever have a question about what should go into a cell, hover over the...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...in March 2017 and updated by Angela Holmes and Christina Mork in February 2024.    Additional Resources...

...as a creative problem-solving exercise? Asking questions like the below can help you understand the perspective... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ... How can I negotiate to show the company that investing in me is also beneficial to them? I have an idea...

...within a multi-tRNA synthetase complex, the team investigated whether the generated antibodies could bind ...myo-inositol oxygenase), providing enzyme protection while still allowing substrates and products to diffuse freely...encapsulated enzyme produced 20% more D-glucaric acid, suggesting that MPyV system can improve metabolic pathway... a ready-to-use viral prep in serotype AAV1. Distinguish dendritic neurites from axonal neurites with ...

...kinds of CRISPR tools people were depositing and requesting each year (Figure 2). We did a similar analysis...2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests ...continue to develop and share new tools, while our requesting labs continue to request and use new tools. Because... each country. Countries shown are the top 10 requesting countries. FR=France; KR=South Korea; CH=Switzerland...

...have specific web sites for postdoc listings, so the method of posting varies between companies. Like most...that they were having a positive experience, I was still incredibly nervous about leaving academia. A lot...seen listed as a requirement on some industry job postings. Also, if you work in industry for any length ...