We narrowed to 731 results for: Sis;

1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   ...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

2. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   ...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...

3. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   Blog Post

   ...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

4. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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   Blog Post

   ...neuroscience research. More broadly, advances in gene synthesis, affinity reagent screening, and next-generation...

5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   Blog Post

   ...fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP...

6. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   Blog Post

   ...Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin and antibodies with crystalline...

7. Donations from Addgene to Yield Answers for Rare Disease Researchers

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   Blog Post

   ...AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied...

8. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   Blog Post

   ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

9. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   Blog Post

   ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

10. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post

    ...became aggressive. If we can understand the neural basis of violence or aggression, it might do a lot to ...

11. Kit Free RNA Extraction

    Type

    Protocol

    ...general steps of this protocol include homogenization/lysis of cells or tissues, extraction of RNA, precipitation...isolation will depend on how effective your cell lysis protocol is. While simple homogenization is effective...

12. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post

    ...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

13. K. phaffii: Rising to the Occasion

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    Blog Post

    ...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

14. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post

    ...technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,...

15. FPbase: A new community-editable fluorescent protein database

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    Blog Post

    ...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...

16. 25 Work From Home Tips for Scientists

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    Blog Post

    ...communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ...

17. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post

    ...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making...

18. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type

    Blog Post

    ...report is that when it became clear that this assistive reproductive technique was feasible, three separate...

19. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    Blog Post

    ...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ...

20. Networking Follow-up: I Met a Person. Now What?

    Type

    Blog Post

    ...touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked...

21. Celebrate National Book Month with Addgene

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    Blog Post

    ...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're...

22. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    ...., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ...

23. Pushing the Limits of DNA Assembly

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    Blog Post

    ...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

24. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    ...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution...

25. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type

    Blog Post

    ...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

26. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post

    ...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

27. Components of CRISPR/Cas9

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    Blog Post

    ...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

28. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

    ... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

29. Management for Scientists: Delegating is Key

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    Blog Post

    ...new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage...

30. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    ...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset...

31. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    ...polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene...

32. Viral Vectors 101: Biosensors

    Type

    Blog Post

    ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

33. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    ...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

34. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    ... for understanding how the brain functions. The basis for these connections in the brain are (drum roll...

35. How Addgene Creates Content

    Type

    Blog Post

    ...reviews on blog posts are done on a case-by-case basis, as many of our blog contributors primarily write...

36. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...

37. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

38. Plasmids 101: Inducible Promoters

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    Blog Post

    ...present, YFI is inactive, preventing repressor cI synthesis and allowing reporter gene transcription to take...

39. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post

    ...seem to require some mathematical know-how for analysis. If you’re looking to get into neuroscience and...

40. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    ...2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike...