We narrowed to 731 results for: Sis;

...fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP...

...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

...many immature neuron-like qualities, which is inconsistent with kidney cells (Shaw et al.). Since then,...

...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...

...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

...neuroscience research. More broadly, advances in gene synthesis, affinity reagent screening, and next-generation...

...Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin and antibodies with crystalline...

...AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied...

...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

...became aggressive. If we can understand the neural basis of violence or aggression, it might do a lot to ...

...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...

...., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ...

...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution...

...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

...technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,...

...communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ...

...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

...report is that when it became clear that this assistive reproductive technique was feasible, three separate...

...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making...

...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're...

...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ...

...touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked...

...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

...new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage...

...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset...

...polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene...

...chromatin-related plasmid in a collection that grows on a daily basis, the lists below provide a snapshot of relevant ...

...present, YFI is inactive, preventing repressor cI synthesis and allowing reporter gene transcription to take...

...seem to require some mathematical know-how for analysis. If you’re looking to get into neuroscience and...

...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

...2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike...

... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

... for understanding how the brain functions. The basis for these connections in the brain are (drum roll...

... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific...