We narrowed to 808 results for: Eng

...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...

...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...

...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...

... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...

...and feedback giving authors the opportunity to strengthen the manuscript prior to submission. Preprints...

...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...

...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...

...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...

...working with communities, not for them - Another strength of this collaboration is that it is driven by ...

...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...

...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...

...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...

...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...

...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...

...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

...outside of the reader’s field of view because of its length, and will be more difficult to read. The Thompson...

...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...

...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...

...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

...Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox...

...Ceballos-Diaz, C., Liu, X., Paterno, G., Mejia, C., Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos...

... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

.... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...

...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...

...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...

...colleagues to reduce off-target hits in a rationally engineered Cas9 with improved specificity. Hold this flexible...

...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...

...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...

...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...

...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...

...included in the search to maximize the alignment length. While this recommendation is not infallible, we...

...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...

...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...

...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...

...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...