We narrowed to 1,092 results for: addgene
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TypeBlog Post...can also find many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure...Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now that you’ve...PMCID: PMC3969854. Additional Resources on the Addgene Blog Get the Basics of Mouse Genome Engineering...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...
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Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog Post... to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench series was born from the... Various Addgenies... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post... the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where... riboswitches used in the Riboglow platform at Addgene. Braselmann E, et al. Nature Chemical Biology... Various Addgenies... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...plasmids from this publication at Addgene! Additional Resources on the Addgene Blog Find planarian tools for...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...understand how therapeutic strategies work. Find Addgene Blog posts about other educational tools Playing...twitter @FRE_SCI. Additional Resources on the Addgene Blog Find planarian tools for teaching developmental...Arabidopsis Biological Resource Center Resources on Addgene.org Find molecular biology educational resources... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various important considerations...github @tlambert03. Additional Resources on the Addgene Blog Fluorescence Microscopy Techniques - Which...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...Kamens is a science communication summer intern at Addgene and a rising third-year at Old Dominion University...-vs-gram-negative-323007 More resources on the Addgene blog Plasmids 101: What is a plasmid? Plasmids ... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...10.1261/rna.069286.118 Additional resources on the Addgene blog Find more molecular biology and viral vector... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...Fatima's work and keep checking back in with the Addgene blog for future award opportunities. Xin Zhou:...Chemical Biology! Additional Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein Biosensors Browse Plasmids... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...CRISPR Editing Additional resources on Addgene.org Addgene's CRISPR Guide (Jump to Large Edits) CAST...10.1126/science.adt5199 Additional Resources on the Addgene blog INTEGRATE: Bacterial Genome Engineering ... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...Scientists Series Resources at Addgene.org Learn About Careers at Addgene ...need to get there. Additional Resources on the Addgene Blog Learn about Possible Science Careers How ... -
Science Career Options
TypeBlog Post...Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...perfect application. Additional Resources on the Addgene Blog Learn How to Experiment with New Careers ... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...writing a scientific review article Write for the Addgene Blog ... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...multi-color imaging. Find the HA frankenbody tool-kit at Addgene to get started with your experiments! What else...PMCID: PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover ...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...EYFP (55645-AAV5) Additional resources on the Addgene blog Read all of our viral vectors blog posts ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...can be deposited in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories...PMCID: PMC4528400. Additional resources on the Addgene blog Find blog posts on scientific sharing Get...tips on writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication... -
Plasmid Cloning by PCR
TypeBlog Post..., you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer, to allow you to identify which...the final product. Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch...Tips on Verifying Your Plasmid Resources on Addgene.org Check out Our Plasmid Reference Pages Deposit... Various Addgenies... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...innovative biological and translative technologies. Addgene has empowered researchers to harness previous experimental...For more information on CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your... -
2022 Depositor Week
TypeBlog Post...an eye out there for a chance to win some cool Addgene swag. And, of course, to all our depositors: thank...2022! It’s that special time of year where we Addgenies use our social media and blog to say thank you... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...collaborative scientific effort. As we like to say at Addgene: sharing speeds science. Here, we’d like to highlight...and we’re grateful for every scientist who uses Addgene to share their tools. We can’t wait to see what...10.1126/science.aba8853 Additional resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors...PAM-Free CRISPR CRISPR in the Clinic Resources on Addgene.org Browse CRISPR plasmids for base editing Read... -
CRISPR 101: Off-Target Effects
TypeBlog Post...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post... of a tagged protein. Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...10.3390/ijms21176324 Additional resources on the Addgene blog: Read about frankenbodies, an anti-HA scFv-based...neuroscience research from the Trimmer lab Check out Addgene's latest project to develop an open antibody resource... -
Five Popular Model Organisms
TypeBlog Post...to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model organisms is...in C. elegans plasmids and resources check out Addgene’s Worm Expression page. Looking for more model organisms...PMCID: PMC2666344. Additional resources on the Addgene blog Read our quick guide to Drosophila Download...Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection pages Read our... Various Addgenies... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, 2.1 plasmids...addition to the neuronal pThy1-Brainbow constructs, Addgene also has two Brainbow adeno-associated viral vectors... -
DAP arrays for multiplex gene editing
TypeBlog Post...detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716...References and Resources More resources on the Addgene blog CRISPR 101: Multiplex expression of gRNAs ... -
Antibodies 101: ChIP
TypeBlog Post... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ... References and Resources More resources on Addgene’s blog Antibodies 101: Immunoprecipitation Antibodies... -
Pushing the Limits of DNA Assembly
TypeBlog Post...obtaining a plasmid with the gene of interest (search Addgene plasmids by gene here), designing primers with ... profession. Additional Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly...Plasmids by Colony PCR Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production. ...PMCID: PMC5538794. Additional Resources on the Addgene Blog AAV Titers: Where do they come from and what...Expression in Mammals Additional Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post... the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral...Viral Service Resources on Addgene.org Visit Our AAV Guide Page Find Ready-to-use Virus for Your Research... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post... not yet available, just ask an Addgene scientist at help@addgene.org! References and Resources References...10.1038/nprot.2017.016. Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How...Lentiviral Transduction Additional Resources on Addgene.org Plasmid Pooled Libraries Pooled library amplification... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...external collaborations Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ...mahesa2504 Resources Additional resources on Addgene's blog Check out our blog post about remote collaborations... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post... on Addgene.org Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics...BioRxiv (2019): 739201. Additional resources on the Addgene blog Learn about using nanobodies to control protein... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR...PubMed PMID: 30377374. Additional resources on the Addgene blog Download our CRISPR eBook Learn about fluorescent...tools Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR plasmids for visualization... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...PMCID: PMC5374486. Additional resources on the Addgene blog Read all of our viral vectors blog posts ... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera... 36.9 (2018): 819. Additional resources on the Addgene blog Learn about other CRISPR methods for bacterial...Mutagenesis to mutate your plasmid Resources on Addgene.org Browse our CRISPR guide pages Find bacterial... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...you to meet..... *drumroll* Aavery! Welcome to Addgene, Aavery! Original post: In December, we... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...stellar resume and cover letter can be found on the Addgene blog. 3. Personal statement brilliance In addition...co-founder of STEAMid. Additional resources on the Addgene blog Check out other tools and resources for early... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics:.../help/spectral-overlap/ More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene .... References and Resources More resources on Addgene’s blog Antibodies 101: Introduction to Immunofluorescence... -
Antibodies 101: Isotypes
TypeBlog Post...an Antibody animation Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...www.ncbi.nlm.nih.gov/books/NBK27162/ Additional resources on the Addgene blog Learn about the advantages and disadvantages... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...mVenus as a reference protein is available from Addgene. Another way to dodge cellular variation observed...based biosensors. Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...measure luciferase output at different wavelengths. Addgene provides empty backbones with the luciferase gene...July 2025. Resources Additional Resources on the Addgene blog Luminescent Imaging with Nano-lanterns Technologies...Proteins 101: Introduction to FRET Resources on Addgene.org Luciferase collection page Promega Plasmid Collection... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post... LIC received an important update, courtesy of Addgene depositor Stephen Elledge. His new method, named.... PubMed. JBEI-J5’s guide to SLIC Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly... -
Visualizing Protein Turnover In Situ
TypeBlog Post...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post... Click Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration...PMCID: PMC1368960. Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie...Lentiviral Transduction Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection ...10.1101/pdb.top099101 Additional resources on the Addgene blog Read about another common type of antibody... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted.../s41587-020-0453-z Additional resources on the Addgene blog Browse our blog posts on base editing Read... in our CRISPR 101 blog series Resources on Addgene.org Find base editors for your research Read our... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology blog posts Read about synthetic...Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...