We narrowed to 430 results for: {{{s}}}
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TypeCollection...Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory R egion) plasmids... in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc Firefly Insertion of...
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FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...PubMed PMID: 24727401. Bernstein, Jacob G., and Edward S. Boyden. "Optogenetic tools for analyzing the neural... PubMed Central PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted... -
Arf GTPase Family
TypeCollection...Cherfils, J., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi... -
TALEN Guide
TypeCollection...from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post... Plasmid Serotype Name 50363 AAV5 AAV phSyn1(S)-DreO-bGHpA 121675 AAV9 pAAV-EF1a-fDIO-Cre (FLP-dependent... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post... non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...DNA repair. A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...