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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 730 results for: ACE;

Showing: 281 - 300 of 730 results

  1. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a.m. many ...format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease case...they agreed on each element to use, they had to place the selected card on the board game. They had 35...

  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs developed...discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4...genes identified as non-essential. The knockouts replace entire protein-coding regions (excluding start ...

  3. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...cell!)—ATP. There are biosensors for intracellular, extracellular, and even the ADP:ATP ratio. Most of... from dopamine to glutamate, all the way to acetylcholine. These sensors have been tested in vivo, in ...

  4. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...substrate, they synthesized diacetyl coelenterazine-h. Unlike coelenterazine-h, diacetyl coelenterazine-h does... to the media. Cellular esterase then converts diacetyl coelenterazine-h to coelenterazine-h, producing...

  6. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...One of the most pivotal decisions many PhDs face when transitioning from academia to industry is whether...requires deliberate preparation and a willingness to embrace new responsibilities. After my second postdoc,...permission to explore, reflect on your priorities, and embrace these transitions as an integral part of your broader...

  7. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...guided by a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...

  8. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown...dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor...

  9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...forms tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage...predicted with no addition of nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either...once, and each <200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate...

  10. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ... There is essentially no better place for a scientist to make new relationships than at scientific conferences...rut of hanging out with only your lab mates (let’s face it, you see enough of them).  Before the conference...students and postdocs. There are many inexpensive/free places to order cards. There are a few good reasons to...

  11. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...biggest difficulties that plant biotechnologists face is public perception of genetic modification (GM...and we’d love to hear about the obstacles you’ve faced as a plant scientist, and how you were able to overcome...Bioengineering Follow RevBio on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic...

  12. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were even ...into M phase oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that...articles and commentaries) pop up on Twitter and Facebook, shared by biologists and non-biologists alike...

  13. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...quicker to pick up new languages!) Jargon has a place in science communication, but the more you use, ...of detail Again, this is an obvious one on the surface, but it’s quite easy to add in “necessary” detail...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed. The ...

  14. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under the control of...needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a constitutive promoter...production titers decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans...

  15. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...talked with Voigt about this collision that’s taking place between CRISPR technology and synthetic biology,.... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental... Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 ...

  16. Working with Nuclear Receptors

    Type
    Blog Post
    ...the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely...

  17. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

  19. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ... like cliff notes, so I use these as a starting place for drafting my stories. In total, I prepare 5-6...arrangements with those you live with to have a quiet place to take your video call.  Expect technical difficulties...

  20. A Look at Addgene's QC Process

    Type
    Blog Post
    ...indicated by exclamation marks. Error points include places where DNA is being created, such as PCR amplification...request the lab provide a new, sequence-verified replacement for the plasmid, and the full QC process is started... started over again after receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s ...
Showing: 281 - 300 of 730 results