Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 970 results for: Abo;

Showing: 281 - 300 of 970 results

  1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...for Scientists Series. I have been thinking a lot about Mentoring for over 10 years. Many successful scientists...Medical School researcher Connie Cepko to learn about her mentoring style. While working on this project...postdocs I have started to talk to trainees more about peer mentoring. They want to find mentors to help... questions I get is, “What are we going to talk about?” There are an infinite number of ideas on the internet...from a lab, career or industry of interest – learn about their career paths and get their advice. Attend ... your own mentoring group or with any questions about mentoring. Read the other posts in the series to...

  2. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...work if both parties are diligent about replying and thinking about good questions and answers. Professional...Sometimes, it is not who you know but who knows about you. I found some other great tips for finding mentors...Medical School researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We...Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. If your organization...much less threatening! If they say no, don’t worry about it. It is a compliment to ask someone to share their...

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...is referred to as an antigen. You can learn more about antibodies in our Antibodies 101 animation, What... expressing this protein. Read on to learn more about antibodies and how to use them in the lab! Parts...lots is a big concern. You can find more details about polyclonal antibodies on our Antibodies 101: Polyclonal...not necessarily what you are getting. Learn more about monoclonal antibodies here. Recombinant antibodies... as well as compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies...Additional resources on the Addgene blog: Read about another reagent sharing platform for antibodies,...Developmental Studies Hybridoma Bank Learn more about nanobodies Watch our Antibodies 101 animation, What...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...biosafety cabinet with cloning rings or following labor-intensive limiting dilution protocols? If this sounds...fluorescent microscopy must be used. To learn more about the most suitable fluorescent microscopy technique...a laser and the instrument collects information about the cell’s size, complexity and fluorescence intensity...captured by detectors and provides useful information about the cell. Light that continues in the same direction... forward scatter (FSC) and provides information about the relative size of the cell; larger cells produce...called side scatter (SSC) and provides information about the cell’s complexity. A cell with a high degree...other methods using fluorescent proteins Learn more about antibodies Resources on Addgene.org Visit the ...

  5. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ... careers, which she hadn’t.  He then said, “How about being a science writer?” to which Susan replied,...replied, “What’s a science writer?” Learning about SciComm by doing SciComm Writing had always been a strength...the National Eye Institute (NEI), where she wrote about NEI research for a general audience. She then got...always plenty of writing: plain-language stories about Fred Hutch research, pieces for donors such as fundraising...articles are just harder to write. “There’s something about being a writer that can be a very painful process...using social media as a scientist here Learn more about storytelling in science ...

  6. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...differently based on their audience. How you talk about your experiments to your PI, your colleagues, or...have cross-team projects within your own lab, collaborations with other scientists within and outside your...not happen in the lab, they have parallels to collaborative lab work. Corralling context and junking jargon...difficult to know what kind of plate they were talking about during the meetings. And “plate” certainly wasn’... a lot on context. New people in the lab or collaborators will not have the context you do, which means... Additional resources on the Addgene blog Read about other transferable skills Find all of our science...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ... the best way I’ve come up is this: It takes me about the same amount of time to read a 100-page genre...it instead as valuable feedback.  Listen. Think about the questions you’ve received, read the comments...aware of cognitive load changed the way I thought about engaging with learners. If something I was communicating... instead of blaming the audience, I would think about how I was presenting the information. Maybe I needed...observe and adjust on the fly.  Once you can think about everything you’re asking your learners to do, it... engagement with your target audience. Thinking about cognitive load will help you understand why you’...

  8. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... time, when someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD...mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize...for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments...2019). Structural diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    ...20th anniversary was about all the amazing years behind us, as much as it as about the amazing years in...Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning...Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016, we also... As the pandemic slowed, Addgene began to think about how we could expand our support for the research...part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative....

  10. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in my head...doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week!  Nerves...attention? fear of messing up?–and use the three tips above to make a strategy that can help. Even something...speaking as a transferable skill The worst thing about doing public speaking as a scientist is that you...perfectly, you truly are a rock star.  The best thing about public speaking as a scientist is that you will ...increases visibility of your work, opens up collaboration and growth opportunities, and gives your entire...

  11. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Individual Development Plan Learn a Little about The Academic Labor Environment from the Future of Research...research.  It helps to spend some time thinking about what your goals are for the coaching relationship...ask for references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will have...with someone you connect with. Talk to the coach about their “style” and make sure this is a style that...coaching group is Still Point Coaching. One nice thing about a group is that sometimes this offers the option...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...Sainsbury Laboratory. She is passionate about science communications and loves talking about science, ...Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early career researcher...break into science communication? Everybody goes about this in their own way. The career paths into science...Society gives training in media skills, writing about your research and residential communication. If ...

  13. Lab Automation at Addgene

    Type
    Blog Post
    ... work into finding robots that’ll take a little labor off our hands. Good news! There is probably a machine...96 transformations in one run compared to doing about 50 transformations in the same amount of time by...machinery we have been able to transform 96 samples in about 4 hours with a greater than 95% success rate! Automating...plates in just under 3 hours or one 96 well plate in about an hour. Automating vial fiIling The majority of...35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand. How can you start...on each tube by hand. We’ll be sure to tell you about more lab updates as we develop them. In the meantime...

  14. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...ask the depositing laboratory to review the discrepancies. If the depositing laboratory confirms that these...finish the library preparation process only takes about one day, even for six plates (576 individual plasmids... we perform a 2x251 run on our MiSeq that takes about two days to complete. After the run completes, we..." In some cases, there will be more information about our sequencing results in the Depositor Comments...to note the appropriate barcode for each sample. About 24 hours later we unload the FASTQ data from our...

  15. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...distance so ideal implant placement should be about 0.4-0.5mm above the site of your stimulation. The scatter...scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that the majority...instead). Laser stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in...These lasers are relatively cheap and will run you about $2-3,000/laser. However, an important caveat is ...output of the laser must be controlled. As discussed above, each opsin variant has slightly different activation...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Plasmid cloning is an essential...with successful recombination reported in all laboratory E. coli strains tested so far. The use of this...low cost and can be immediately adopted by any laboratory. All procedures are performed using the same... Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral...postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology. He is currently investigating...García-Nafría is an investigator scientist at the MRC Laboratory of Molecular Biology. He is interested in neuronal...

  17. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...Control Protein Activity Learn more about protein tags Read about E. coli strains for protein expression..., if not better, than antibodies for all of the above listed molecular techniques, but they can also be...protein purification methods. Read on to learn more about nanobodies and how their structure and function ...between a fluorescent tag and the target antigen to about 2 nm, which can lead to higher resolution for super-resolution...nanobodies. If you’re interested, you can learn more about how nanobodies are identified in this review. If...

  18. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...early employees spent this remaining time by elaborately pranking each other, dressing in costume, and...frequently ask the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all day long, whistling...megaphone, or your company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring...company.        Additional Resources Read other posts about life at Addgene!Read more of our careers posts! ...

  19. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...on becoming grant writers. When we asked others about how they became grant professionals, mid- to late-career...late-career grant professionals have similar stories about a time when their passion for an organization or... logic models, established partnerships and collaborations, and commitments to and proof of compliance...grants. It is no surprise that the U.S. Bureau of Labor and Statistics shows that employment of grant writers... skills & passion  Skills such as research, collaboration across multiple disciplines, writing, project...development strategy builds on the steps listed above and formalizes your development. You may consider...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ...Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about the viral...resources available from Addgene. There’s a lot to talk about! And by the way, there will be viral vector jokes...injection site, and incubation time. Learn more about the Data Hub in this blog post. Alongside our efforts...SfN2019, stop by booth #2163 to say hi and learn more about Addgene’s viral service. Additional resources on...
Showing: 281 - 300 of 970 results