We narrowed to 345 results for: Ada

1. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

2. Plasmids 101: Knockout/Knock-In Plasmids

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   ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

3. Experimenting with New Careers while in Grad School

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   ...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

5. Getting to Know Expansion Microscopy

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   ...model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins...

6. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ...difficulties in dealing with climate change, the degradation of the U.S. environment, and a decline in the...

7. A Guide to Starting Your Own Journal Club

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   ...group and topic. For example, it may be useful to adapt the second version (multiple presenter, figure by...

8. SciComm: Taking Science to Elementary and Middle Schools

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   ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...are quickly cleared from the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR...

10. AAVs in Retinal Gene Therapy

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    ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and engineered...

11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...given state or point in time. You want to halt degradative processes while maintaining the structure of ...

12. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...fashion. 9. Ask for help My grandma used the old adage “many hands make light work” all the time. She was...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...immunoprecipitation (enChIP). Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H. Sci Rep. 2013 Nov ...

14. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...pages. We guarantee this will save you a lot of headaches down the line! Cash in on gene synthesis Before...

15. An Inside Look into Addgene's Dev Team

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    ...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...

16. Antibodies 101: The Four ELISAs and When to Use Them

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    ...measure antibody levels, scientists have since adapted it for a host of different proteins and small molecules...

17. Plasmids 101: Stringent Regulation of Replication

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    ...Figure 2: Prokaryotic chromosomal replication. Adapted from BioRender template. Since DnaA binding...

18. Plasmids 101: Broad Host Range Plasmids

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    ...contains structural elements that are versatile and adaptable. Variation in ori structure allows plasmids to...

19. Plasmids 101: Codon usage bias

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    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

20. Neuronal labeling with Spaghetti Monster

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    ...smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance probes for light and ...