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We narrowed to 963 results for: App;

Showing: 281 - 300 of 963 results

  1. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...these proteins are quite large — comprising approximately 1000–1400 amino acids — which poses a challenge...-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...), which is especially valuable for regulatory approval and public acceptance. Recently, viral vectors... is where TnpB offers a clear advantage. At approximately one-third the size of Cas9 or Cas12a, TnpB is...viral vectors. Even for advanced genome editing approaches like base editing and prime editing (Molla et...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...

  2. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...This distinct approach means researchers can now select the best tool for their application from among many...incorporate A bases across from abasic sites. This approach resulted in a maximum A → T product purity of ...AYBEv3!). Although AYBE, AXBE, and ACBE do not approach the high product purity of CBEs and ABEs, they...). Several different teams succeeded with this approach around the same time (He et al., 2024; Tong et...on in the base editing field! From CRISPR-free approaches to mitochondrial base editing to base editors...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Depending on the research question and downstream application, one or several of those techniques can be used...your flow analysis. However, certain downstream applications, like an in vivo adoptive transfer of those ...or free amines of proteins. Depending on the application, they can be membrane-permeant or membrane-impermeant...are positive or negative for the dye. The main applications of dye stains in flow cytometry are cell proliferation...with a high resolution for specific downstream applications, while maintaining a clean cell surface, dump...and bind DNA or free amines, depending on the application. Paul Heisig is a Research Associate in the lab...

  4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...cells retain the same color, allowing for the fate mapping of cell populations over time. The ability to track...variety of species, cell types, and research applications. Labeling neurons with Brainbow The Lichtman...neurons in the cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...cell type when crossed with mice expressing the appropriate Cre allele. The Confetti mouse contains the Brainbow...PMC3713494. Ghigo, Clément, et al. "Multicolor fate mapping of Langerhans cell homeostasis." Journal of Experimental...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...experimental system. AAV1 AAV1 was the first viral vector approved for gene therapy (Issa et al., 2023). It may be...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally... 2016). This functionality makes it useful for mapping neural connections. Although derived from AAV2,...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...

  6. A Control for All Seasons

    Type
    Blog Post
    ...secondary antibodies.   Application-specific controls Some antibody-based applications have very specific ...then moving on to controls for antibody-related applications. After reading, you will have a better understanding... unavailable, try substituting with knockdown approaches using siRNA or shRNA. If this alternative is ... alternative, albeit less ideal, verification approaches discussed in the Antibodies 101: Validation post...Secondary antibody specificity  In indirect staining approaches, secondary antibody specificity must also be ...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...you with these, so keep reading! Good luck, and happy optimizing!  Recommended Reading Issa, S. S., Shaimardanova.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...A. (2021). Next Step in Gene Delivery: Modern Approaches and Further Perspectives of AAV Tropism Modification...AAV-Mediated Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined Neural Pathways...Retrograde Serotype Viral Vectors 101: Viral Applications...

  8. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...making it very useful for genetic engineering approaches. Most importantly for this technology, however...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...ssODN) as the donor [6]. The problem with this approach is that, since there is no selection, you will...technology that may be used for a variety of research applications, including making transgenic cell lines and ..., while avoiding the pitfalls common to other approaches. Many thanks to our Guest Bloggers Amar Singh...Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge to help researchers identify...

  9. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...created by the Recipient Right to file patent applications on modifications or inventions Right to publish...can even sign electronically or choose to auto-approve MTAs. Whatever your preference, we are here to ...to make it fast and easy. Fast Approval: The UBMTA used by Addgene is internationally recognized as...transfer officers are familiar with the UBMTA and are happy to agree to it. Their confidence and familiarity...familiarity with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...have used fluorescent proteins to observe what’s happening inside cells for at least a couple of decades....of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...exactly what they reported doing in a paper that appeared in Nature Biotechnology back in 2011 and then ...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670...contrast agents for two-color imaging in animals. The approach relies on ultrasound waves as opposed to light...

  11. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...increase specificity. This modular approach made ZFNs highly appealing to researchers.  TALENs In 2010, ...fiction, was also a trained biologist. This is apparent in his work The Island of Dr. Moreau (1896) where...made SciFi director Ridley Scott wonder what would happen if these corporations would extrapolate genetic...successful in the box office, indicating the insatiable appetite of the moviegoers for dinosaurs wreaking havoc...also transcended into the world of SciFi. This is apparent in the use of the word ‘CRISPR’ for anything associated...

  12. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... Normal Variables – Independent Variance – Approximately equal Data type - Quantitative If your data ...null hypotheses. Benjamini uses a complementary approach which identifies a p value cut off. This method...all generally yield a similar result and are appropriate to use if all sample comparison is needed, without...keep your friendly neighborhood biostatistician happy by making your statistical analysis plan when you...right statistical test, just do your homework and happy testing!   References Wissing, Dennis R., and Timm...Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389/fams...

  13. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...thermophiles, bacteria must be grown at lower temps. This approach only works for facultative thermophiles (high ... occurs? This is different than the engineered approach used to make eSpCas9 and SpCas9-HF1. By mining...SpCas9 RNP’s and a guide targeting a site with an overlapping PAM. In both cases, GeoCas9 and SpyCas had similar...could also be used with in vitro molecular bio applications that require high temperature reaction conditions...always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al....

  14. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...scientific aspects of writing. Following his advice, I applied to the class. Receiving the email that I had been...the ASM headquarters. I decided to go ahead and apply; thinking I probably would not get selected for ...knowledge with practical understanding of how to apply it. The personal interaction of critiquing others...well as being critiqued added an extra level of application not present in the online class.   Four ways ... manuscript should be able to be enjoyed and appreciated by all fields not solely the specific area the...

  15. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...learn how easy this process is and to get tips for applying it in your own lab. Table 1: Comparison Between... E. coli and transformants selected with the appropriate antibiotic. Although the precise mechanistic ...additional 15-17 bases that are complementary to the overlapping vector ends. See an overview of the PCR process...an agarose gel. You should see bands with the appropriate sizes for your vector and insert. Once you’ve...correct product. Once you find a colony with the appropriately sized product, purify and sequence the plasmid...

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted... subcellular regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting...to the limits of light delivery. Chemogenetic approaches excel at broad tissue or system-wide targeting...chemogenetics. Note that, as with all tools, both approaches suffer from unique sets of off-target side-effects...EndogenousNot easily controlled Unique advantages Mapping the brainDissecting circuits Studying GPCR pathways...

  17. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...tag to remain on your protein for downstream applications Below, we describe a few of the most common...not to perturb protein function for downstream applications. Tag cleavage must be performed after primary...also provides a visual detection mechanism via applications such as western blot and immunofluorescence....dimerizes in solution, which can affect downstream applications. Also, purifying GST by affinity chromatography... are generally best suited for their primary application. Ready to start tagging? We are here to help ...

  18. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...users have recently begun to develop CRISPR-based approaches for studying gene function in these systems. ...use in a fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived...tissue-types. By bringing together CRISPR-based approaches and organoid models, toolkits like this one provide...trigger seed germination of parasitic plants, this approach was also used to build and validate a ratiometric...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...

  19. What the HEK?

    Type
    Blog Post
    ...isolating the cell line all the way to modern applications. What the HEK is it?   HEK cells were first...cell biology, there are thankfully many other applications that they are useful for! What the HEK is it...cell line and we will highlight some of those applications here.   Virus production HEKs are the go-to ...proteins at high levels. In a case where clinical applications of recombinant proteins are necessary, HEKs ...significantly reduces the cost of production. Research applications There are two areas of research where HEK cells...

  20. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...NgAgo replace CRISPR? Such a drastic shift won’t happen overnight, but there are a few reasons why you ...flexibility in choosing a genomic target. NgAgo also appears to outperform Cas9 in GC-rich regions. DNA guides...staggered (Cpf1) cleavage. NgAgo takes a different approach, randomly removing 1-20 nucleotides from the cleavage...site specified by the gDNA. Depending on your application, this could be favorable or unfavorable. In a...activity. One-guide faithful NgAgo guide loading appears limited to the period when the protein is being...
Showing: 281 - 300 of 963 results