...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

...from the Zhang group . Many protocols have been published with thorough step-wise instructions on designing...references. For genome editing, the Zhang lab has published this Nature Protocol article . Should I add the...very robust in this case for EMX1. Since the publication of our paper, we have two new optimized primers...primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the reaction...

...reagents, we scaled up these tagging experiments by assembling csRNPs in a 96-well plate format and tested parallel...Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. ...

...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...

...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

...Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene...Gene/Insert PI Publication Do you have suggestions for other plasmids that should be added to this list?...

...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid ...Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January 30, 2025 Do you have suggestions for other...

...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase...

...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...

... for Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger...Scientists using these zinc finger arrays in a publication should notify [email protected] and...acknowledge NIH grant number R01 GM088040 in the publication. References Rapid 'open-source' engineering of...

...cloning chassis and set of functional parts for assembling plant transformation vectors - Daniel Voytas....community. Grassius (Link opens in a new window) - a public web resource composed by a collection of databases...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require cloning of left and ...the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences (N153AA, C63AA) and showed...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...

...factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic cells. A common...be found at Addgene and in Figure S12 of the publication. Agudelo D, et al. Nature Methods. 2017. PubMed...

...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...or follow the references to find the original publication and ensure that it includes validation data relevant...

...from three separate plasmids A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult...differentiation of iPSCs into cortical and motor neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons...and more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...