We narrowed to 403 results for: CAPS

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ... delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...

2. Plasmid Tools for Microbiome Studies

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   ...Pamela Silver’s lab developed a synthetic mark and recapture strategy for bacteria to count cell divisions....

3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

4. Developing Lab Management Software for Biology

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   ...materials make everyone's life easier, the time and capital cost of getting up to speed with a setup is almost...

5. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...

6. Antibodies 101: Conjugation

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   ...-based assays. Biotin itself is not visible or capable of producing a visible signal, but it has a strong...

7. To Each HIS Own

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   ... level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended if ...

8. Plasmids 101: Golden Gate Cloning

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   ...precision cloning method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One...

9. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...transition is also an area of specialty for Michelle Capes at Adeptify Me. While based in Madison, Wisconsin...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure. This method...

11. How to Prepare for an Industry Interview

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    ...a theme too, which is something not inherently captured by the STAR method. I like to think of the theme...

12. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    ...focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers...

13. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling ...

14. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...Sequencing (CPS) workflow and yield sufficient data to capture the entire viral genome. The full details of that...

15. Design Tips for Prime Editing

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    ...but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation...

16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative...

17. Making CRISPR Plasmids Using Fragmid

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    ...Sizes of fragments are not to scale. Figure and caption from McGee et al., 2024, used under the Creative...

18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...the authors tracked protein activity while also capturing information about spatial and temporal organization...

19. CRISPR in the Clinic

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    ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

20. Viral Vectors 101: Calcium Sensors

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    ...studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References...