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We narrowed to 644 results for: CAR

Showing: 281 - 300 of 644 results

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...groups of trainees at an earlier point in their careers? A CURE for Biochemistry Undergraduate biochemistry... then proceed to use those skills to design and carry out their own novel experiments in semester two ...as they increase student interest in scientific career paths while also broadening access to experiences...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... settles in and people get the time recurrently carved out. This notice typically should include the paper...suited for different applications and should be carefully chosen for each group and topic. For example, ...purpose, journal clubs should be well planned and carefully organized. Most importantly, they should be regularly...

  5. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...micro, and cell biology are often more suited for cartoons or graphics for visuals. However, it’s much more...need text to properly contextualize. Graphics/cartoons (no words) Low Visually compelling and flexible...requires both artistic and scientific training. Cartoons may be read as “for children” or may need text...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    ...bacterial CRISPR targeting. Phage-derived vectors carry CRISPR RNAs (crRNAs) targeting either genomic (blue... integrating into the bacterial genome, and it carries CRISPR machinery targeting 1) the given resistance...hardest-to-kill bacteria. In using phage-derived vectors to carry CRISPR, we’re turning the tables on the bacteria...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...vector can protect both plasmid-carrying bacteria and eukaryotes. But be careful — antibiotic mechanisms vary...and jump between all kingdoms of life. A bit of careful design will help make your experiment with shuttle...

  8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...generating interesting drug-like compounds especially cardenolides which have been used to treat heart disease....disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous) for bird ...system (Receptor-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the arthropod germline by...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Cultures should only be passaged 20-30 times before discarding and thawing a fresh vial. In many labs, the history...Routinely test your cell line for mycoplasma and discard it if the culture has become overly confluent or...ensure high quality and reproducible results always care for your cell lines and virus properly and be consistent...

  10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ... a western blot — and why you might want to be careful relying on so called “housekeeping proteins.” Fully...approach is so common that we’ve used it for our cartoon gels so far. However, it throws both assumptions...Convenient! Comparison of loading control approaches Cartoons are nice and all, but we’ll leave you with this...

  11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...’s which allow researchers to deliver oversized cargo (Hirsch et al., 2010). Today’s blog post will give... https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho LS, Turunen HT, Wassmer SJ, Luna-Velez MV, Xiao...transduction following recombination between AAV vectors carrying parts of a larger gene. Nat Biotechnol 20:697–...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...in vitro translation system. The gene set was carefully chosen to perform statistically relevant benchmarking...conveniently performed through the N-terminal eGFP tag carried on all constructs. The Cell-Free Protein Expression... 48 plasmid adapter library, with each plasmid carrying a unique barcoded Illumina-M13F bridging primer...

  13. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...double-stranded RNA. They hypothesized that if ADAR1 were carried by extracellular vesicles (EVs), edited transcripts... as a sensitive reporter for tracking proteomic cargo flow via EVs. Find ADAR-HiBiT Fusion Vector at Addgene...the heterogeneity and spread of hepatocellular carcinoma in human tissue and in vitro. Oncology Reports...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate... B., Fong, D. M., Mouravlev, A., Young, D., & O’Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy....2014.00076 Nieuwenhuis, B., Haenzi, B., Hilton, S., Carnicer-Lombarte, A., Hobo, B., Verhaagen, J., & Fawcett...

  15. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...al., 2009), and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to...0092-8674(84)90060-6. PMID: 6323019. Williams JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design... JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA production platform incorporating...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...latter would likely be more effective, so long as care is taken in the interpretation of results by requiring...use of CRISPR technology will always depend on careful experimental design, execution, and analysis. ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...

  17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...health sector, phages represent a reservoir of carbohydrate binding proteins waiting to be explored and ...spot this protein directly onto nitrocellulose and carry out the steps from blocking the membrane to probing...bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion in structural biology 34 (...

  18. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...lesser than the mean of a control group. If you only care if one group is different in a certain direction...rate by only performing the comparisons you really care about. Family-wise error is the probability of a...Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume...

  19. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to differences in the ...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for... 12. https://doi.org/10.3389/fnsyn.2020.00006 O’Carroll SJ, Cook WH, Young D (2021) AAV Targeting of Glial...

  20. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... compartments in in vivo model systems, so extra care must be taken to assess if proper staining is achieved... such as conjugation with proteins, should be carefully managed. Proper dye-to-protein ratios, or the ... and quenching, which can be mitigated through careful selection and optimization. Fluorescent experiment...
Showing: 281 - 300 of 644 results