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We narrowed to 649 results for: CHI

Showing: 281 - 300 of 649 results

  1. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    .... DNA quantity is fairly self explanatory and achieved by performing different sized preps, such as miniprep...of your plasmid prep is by spectrophotometry. Achieving the highest-quality DNA requires taking care at... are designed to work together — no mixing and matching!   Figure 2: Plasmid isolation workflows...

  2. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and implanting...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute... three germ layers, and the ability of theses chimaeras to produce all-iPSC derived offspring demonstrates...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...strength, and binding (e.g. DHS-seq, FAIRE-seq, and CHIP-seq), genome-wide identification and quantification...to test multiple expression systems before they achieve the appropriate expression level for their gene...cloning process alone involves much literature searching, DNA synthesis, and plasmid assembly. Luckily,...genome-engineering endeavours of unparalleled complexity in Escherichia coli, like the construction of a so-called “genomically...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells, ...

  5. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...incorporates a bioluminescence protein into the chitin that can be activated only by a specific frequency...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....

  6. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious...:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition...

  7. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...organizing Halloween festivities for years – searching our photo archives I found pictures back through 2009, ...

  9. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering...colossal 547 years! While we are very excited about reaching this milestone, it wouldn’t have been possible...

  10. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...

  11. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk...Fireplace from the Dyer Lab If you’re feeling as chilly this winter as the Addgenies are in Massachusetts...

  12. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... your own depositor metrics!  About the Author Joachim Goedhart is an assistant professor at the Section...fluorescent probes. You can follow him on twitter: @joachimgoedhart.    ...

  13. Editor's Choice, July 2016

    Type
    Blog Post
    ...was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the entire blog and ... Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always...

  15. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...establish.These funding decisions will have far-reaching consequences throughout the scientific community... can turn to Addgene as a trusted partner for archiving their biological materials during funding transitions...

  16. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this year...

  17. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

  19. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...
Showing: 281 - 300 of 649 results