We narrowed to 310 results for: Cad

1. Mesothelioma - Causes, Symptoms, and Treatment

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   Blog Post

   ...tumors.  This process is not quick. It can take decades from the first exposure to asbestos to the time...

2. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...stomatitis virus.” Proceedings of the National Academy of Sciences 110.18 (2013). 7306-11. Pubmed PMID...

3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   ...fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004): 10554-10559. PubMed ...

4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...distinct populations." Proceedings of the National Academy of Sciences 109.2 (2012): 466-471. PubMed PMID:...

5. An Integrin Antibody Toolkit from IPI

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   Blog Post

   ...and migration, initiating intracellular signal cascades, and allowing agile responses to mechanical forces...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

   ...been a staple in molecular biology for over three decades. Widely used across various research applications...

7. Site Directed Mutagenesis by PCR

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   Blog Post

   ...the base sequence." Proceedings of the National Academy of Sciences 83.11 (1986): 3746-3750. PubMed PMID...

8. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

   ...Over the past decade, scientists have developed and fine tuned many different ways to clone DNA fragments...

9. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   ...in complex tissues. Proceedings of the National Academy of Sciences, 117(52), 33455–33465. https://doi....

10. Plasmids 101: Dimers and Multimers

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    Blog Post

    ...multiple circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/...

11. CRISPR Guide

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    Guide

    ...enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is ...separate Cas enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems... by Cas9 systems. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex for a ntiviral ...de fense) complex to initiate DNA degradation. Cascade is made of five proteins: Cas8 (Cse1), Cas11 (Cse2...Overview of editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide variety ...nucleases like Cas9 and Cas12a (also known as Cpf1) Cascade C RISPR- as sociated c omplex for a ntiviral de...Lewis, J. D., & Bondy-Denomy, J. (2020). A compact Cascade–Cas3 system for targeted genome engineering. Nature...

12. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991): 189-193. PubMed PMID: ...

13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...cross-over insensitive ATG-out vectors. Proc Natl Acad Sci USA 116:27001–27010. https://doi.org/10.1073...

14. A History of Genome Engineering in Popular Culture

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    Blog Post

    ... the rediscovery of Mendel. Comptes Rendus de l’Académie des Sciences - Series III - Sciences de la Vie...

15. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    Blog Post

    ...mismatch repair mutants. Proceedings of the National Academy of Sciences of the United States of America. 2003...

16. Sequencing Options for CRISPR Genotyping

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    ...establish standardized validation techniques for academia, industry, and especially the clinic. Popular ...

17. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post

    ...one object in the final image. Over the past two decades a number of techniques have been developed that...

18. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...SpCas9 knockout libraries designed by different academic labs, targeting mouse, human, Drosophila melanogaster...

19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...fluorescence conversion." Proceedings of the National Academy of Sciences of the United States of America 101.45...

20. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...improved cameleons." Proceedings of the National Academy of Sciences 96.5 (1999): 2135-2140. PubMed PMID...