We narrowed to 570 results for: Cit

1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...though that’s what I thought,” she said. “I’m so excited to see where my life will take me. I’m also just...t had these conversations. Now, I am much more excited about where my career path will take me and much...

2. Tips for Titering Your Lentiviral Preps

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   ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

3. The Materials Science of Optogenetics Experiments

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   ...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...

4. How-to: 5 Steps to a Great Panel Discussion

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   ...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

5. Twenty Years of Addgene Sharing: CRISPR

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   ...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story...

6. Viral Vectors 101: Voltage Indicators

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   ...depends on the ability to monitor their signals – electricity! The research tools for studying voltage within...as neuron subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic...

7. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

8. Tips to Make the Most of a Scientific Conference

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   ...done the research, gathered up your data into an exciting story, and are ready to present your findings ...person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout...

9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...in-depth In other tools, guides are ranked by specificity of targeting or by cut-to-insert distance for...determination (CFD score) is used to measure specificity of a guide to the target. The CFD score ranges...

10. Transferable Skills: Negotiation

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    ...Ethiopian?) to where you are going on vacation (New York City or Hawaii?). So how can you approach improving this...starting date with the company so they know I’m excited while still being able to take my vacation? I want...

11. Plasmids 101: Multicistronic Vectors

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    ... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express... Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new eBook...

12. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’...lot. The problem in plant science is there is a paucity of resources. We wanted to expand the toolkit. ...

13. Great Results Start with Great Standard Curves

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    ...of concentrations but suffers from decreased specificity, especially as it approaches the minimum and ...are more disheartening than seeing a potentially exciting result quashed by a bad standard curve. Take the...

14. An Introduction to Adenovirus

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    ... E1 region). The space in the viral particle’s capacity that is made available by this deletion from the...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

15. MXS Chaining

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    ...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular... mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable...

16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...efficiencies of 90-100% (Fig. 2B), and >99% on-target specificity. Additionally, by expressing multiple crRNAs ...the CRISPR toolbox RNA-guided transposases are exciting new additions to the growing CRISPR toolbox for...

17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...where limited expression of Cas9 is required and specificity is a concern, such as knockout generation or ... in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein in the host...

18. Lentiviral Vector Uses and Overview

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    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...

19. Tips for Writing a Good Cover Letter

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    ...nonprofits, who tend to prioritize candidates that are excited about their work and mission.  How do I know what...you excel in the role. It can also convey your excitement for that particular job, your commitment to the...

20. Hot Plasmids - November 2023

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    ...development, transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC...