We narrowed to 821 results for: EED

...the research antibodies in your refrigerator often need a little help to be useful. Mainly because, well...available, that never guarantees the antibody you need will be conjugated, or that it will be conjugated...conjugation, it is important to keep in mind that you will need to confirm your antibodies’ performance after the...which can interfere with conjugation reactions. If needed, you can often remove the incompatible agents by...

...how many do I need? When designing a plasmid containing a polyhistidine tag, you’ll need to decide whether... binding without eluting the target protein and needs to be empirically determined. In addition, some ...Longer histidine tags are also recommended if you need a very pure sample. Because binding strength is ...

...stakeholders. However, while most institutions are indeed going ahead with their planned raises, some are...ahead with the raises as they see fit, following feedback from postdocs and faculty (at time of writing,...detrimental effect on the morale of postdocs, and indeed we have been contacted by a number of postdocs ...keep raising salaries and that we are submitting Freedom of Information Act (FOIA) requests for their postdoctoral...

...is sometimes at odds with the space needed to be creative. “You need a lot of time to let you mind process...usually start out my days looking through our Twitter feeds and dealing with social messages and postings,” ...organizations have so many [science communication] needs. I worked for one company, but there were a ton ...

...projects. Right now we don’t have to worry about succeeding at our core mission. Plasmid distribution is ...this and recognize it all the time. We love this feedback and are always adding to our “google doc of gratitude...awhile I worry a very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year..., excellent intentions, or incredible drive to succeed.  Thanks to all the Addgenies and congratulations...

...designed to support new approaches that address the need to train our young scientists for futures both inside... symposium we will discuss where better data is needed to help shape and inform the overall structure ...graduates and postdocs – and from what fields – are needed to adequately supply these demands? Are there other...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

...of the article – via the journal (though you may need to be on a network with an institutional subscription...literature until you have all of the information you need. Reading primary research literature The best way...feasible for every paper you will ever read. So, you need to come up with an organization system that works...works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search. Writing...

... to see how funds were allocated. Step 3: Get feedback from your peers and advisors Once a rough draft...reach out to peers and collaborators who have succeeded at grant writing to see what tips they found useful...reviewers Reviewers may take 6-12 months to give feedback on the application. Make sure to invest time and...the application process? What types of writing feedback do you find most helpful? Have a great grant writing...

...your western and your results look… funky? Do you need to start a whole new blot? Never fear: membrane .... Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can be returned...antibodies. If you have already applied antibodies and need to dry the membrane for storage, but plan to reprobe...more strongly or that have saturated the blot may need longer incubation for effective stripping. Some ...

...drop-down options for you to choose from, while others need to be filled with short-answer text. You can also...partial sequence cells. *Note: If you believe you need to deposit a collection that is greater than 75 ..., S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic, or other. If...appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping ...

... and skew results measuring locomotion. Mice may need to be given some time to adapt to a testing apparatus...stress level, origin (vendor or in-house mouse breeding colony) Environment Olfaction, temperature, ...possible effects. Injection Parameters: The injection needle should be as thin as possible to minimize the damage... damage to the brain tissue. The injection speed has to be slow to allow the viral particles to diffuse...

...was reported, and we felt that, given the critical need for low-cost, field-based diagnostics, our approach...nights, not unlike most research, but the urgent need for the test did catalyze our resolve to get the...reach out to the greater research community when we needed additional expertise. Perhaps most striking is ...advance our programmable approach to other critical needs and to further develop our rapid prototyping and...

...optics have become go-to providers of materials needed for optogenetics. There are several important considerations...considerations with fabrication of the fiber optics needed for optogenetics. First, the length of the fiber...brings me to the second key point; the fiber optics need to be polished to significant smoothness to prevent...Before you get your experiment started, you will need a laser of the appropriate wavelength to stimulate...

...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ...proteins of interest and proteins tagged with it needed to be chemically treated before the antibody could...recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still...

...precise expression levels. The BIOFAB project is seeding the beginnings of an operating system for programming...genetics and chemistry of life, then this new language needs to be free. The future of synthetic biology Addgene...development of enough sensor domains. Ultimately, we'll need a couple thousand or so sensor domains for everything...could imagine. That I think is the next priority; we need the keyboards and displays for control and visualization...

...rely on standard curves in their day-to-day work. Indeed, viral vector production facilities frequently ...do not know what concentration to expect, you may need to run the experiment a few times and optimize your...contaminants that could affect the measurements. You'll need to prepare the standard, standard dilutions, and...used to measure the response. The dynamic range needs to be wide enough to cover all possible concentrations...

...Professional Scientist,” addresses the challenges of succeeding in science if you’re anything other than a white... cursory manner, their inclusion here adds much needed context to understand every other problem. For ...detract from the quality of the book? It doesn’t need to. The problems and challenges described in The...your life, the book may be the introduction they need to grasp the challenges you face in your career....

...Of course, if qPCR were perfect there would be no need for ddPCR. One issue with qPCR is that the results...a small number of qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning...droplets) can be sufficient to capture the information needed for your PCR experiment. The process to titrate...simultaneously. For AAV titration, you should only need to use one. However, two channels can be used to...

...established grant professionals is learning to succeed in grants through trial and error, through winning...likelihood. We provide expertise on the processes needed to successfully implement, manage and close-out...materials for publishers. When the community orchestra needed a grant writer, Deb volunteered. Through these ...private businesses. How do you get the expertise needed to enter the grants profession? The four steps ...

...delivery system, the specific target cell or tissue needs to be considered. Q2: Can I use lentiviral transfer...When expressing a viral construct transiently care needs to be taken to ensure that the proper cell line ... 5’ LTR of the transfer plasmid to eliminate the need for the HIV Tat protein and consequently are considered...where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids replication...