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We narrowed to 824 results for: EED

Showing: 281 - 300 of 824 results

  1. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...designed to support new approaches that address the need to train our young scientists for futures both inside... symposium we will discuss where better data is needed to help shape and inform the overall structure ...graduates and postdocs – and from what fields – are needed to adequately supply these demands? Are there other...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

  2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...of the article – via the journal (though you may need to be on a network with an institutional subscription...literature until you have all of the information you need. Reading primary research literature The best way...feasible for every paper you will ever read. So, you need to come up with an organization system that works...works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search. Writing...

  3. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... to see how funds were allocated. Step 3: Get feedback from your peers and advisors Once a rough draft...reach out to peers and collaborators who have succeeded at grant writing to see what tips they found useful...reviewers Reviewers may take 6-12 months to give feedback on the application. Make sure to invest time and...the application process? What types of writing feedback do you find most helpful? Have a great grant writing...

  4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ... and skew results measuring locomotion. Mice may need to be given some time to adapt to a testing apparatus...stress level, origin (vendor or in-house mouse breeding colony) Environment Olfaction, temperature, ...possible effects. Injection Parameters: The injection needle should be as thin as possible to minimize the damage... damage to the brain tissue. The injection speed has to be slow to allow the viral particles to diffuse...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...your western and your results look… funky? Do you need to start a whole new blot? Never fear: membrane .... Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can be returned...antibodies. If you have already applied antibodies and need to dry the membrane for storage, but plan to reprobe...more strongly or that have saturated the blot may need longer incubation for effective stripping. Some ...

  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...drop-down options for you to choose from, while others need to be filled with short-answer text. You can also...partial sequence cells. *Note: If you believe you need to deposit a collection that is greater than 75 ..., S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic, or other. If...appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping ...

  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...was reported, and we felt that, given the critical need for low-cost, field-based diagnostics, our approach...nights, not unlike most research, but the urgent need for the test did catalyze our resolve to get the...reach out to the greater research community when we needed additional expertise. Perhaps most striking is ...advance our programmable approach to other critical needs and to further develop our rapid prototyping and...

  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...optics have become go-to providers of materials needed for optogenetics. There are several important considerations...considerations with fabrication of the fiber optics needed for optogenetics. First, the length of the fiber...brings me to the second key point; the fiber optics need to be polished to significant smoothness to prevent...Before you get your experiment started, you will need a laser of the appropriate wavelength to stimulate...

  9. Of Myc and Men

    Type
    Blog Post
    ...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ...proteins of interest and proteins tagged with it needed to be chemically treated before the antibody could...recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still...

  10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...precise expression levels. The BIOFAB project is seeding the beginnings of an operating system for programming...genetics and chemistry of life, then this new language needs to be free. The future of synthetic biology Addgene...development of enough sensor domains. Ultimately, we'll need a couple thousand or so sensor domains for everything...could imagine. That I think is the next priority; we need the keyboards and displays for control and visualization...

  11. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...Professional Scientist,” addresses the challenges of succeeding in science if you’re anything other than a white... cursory manner, their inclusion here adds much needed context to understand every other problem. For ...detract from the quality of the book? It doesn’t need to. The problems and challenges described in The...your life, the book may be the introduction they need to grasp the challenges you face in your career....

  12. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...rely on standard curves in their day-to-day work. Indeed, viral vector production facilities frequently ...do not know what concentration to expect, you may need to run the experiment a few times and optimize your...contaminants that could affect the measurements. You'll need to prepare the standard, standard dilutions, and...used to measure the response. The dynamic range needs to be wide enough to cover all possible concentrations...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...Of course, if qPCR were perfect there would be no need for ddPCR. One issue with qPCR is that the results...a small number of qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning...droplets) can be sufficient to capture the information needed for your PCR experiment. The process to titrate...simultaneously. For AAV titration, you should only need to use one. However, two channels can be used to...

  14. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...established grant professionals is learning to succeed in grants through trial and error, through winning...likelihood. We provide expertise on the processes needed to successfully implement, manage and close-out...materials for publishers. When the community orchestra needed a grant writer, Deb volunteered. Through these ...private businesses. How do you get the expertise needed to enter the grants profession? The four steps ...

  15. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...delivery system, the specific target cell or tissue needs to be considered. Q2: Can I use lentiviral transfer...When expressing a viral construct transiently care needs to be taken to ensure that the proper cell line ... 5’ LTR of the transfer plasmid to eliminate the need for the HIV Tat protein and consequently are considered...where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids replication...

  16. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...of codons, it only needs to produce a subset of charged tRNAs and therefore may need fewer resources for...Adaptation to changing conditions - Organisms often need to express genes at different levels under different...determined by both codon bias and folding energy." Proceedings of the National Academy of Sciences 107.8 (2010...

  17. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...initially created to support the CRISPR analysis needs of Synthego’s scientists, and it’s now free for ...experiments and comparing the robustness, accuracy, and speed with existing tools. For example, we examined ICE...ICE will do the rest. There are no parameters that need optimizing and no complicated steps to learn. For...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...location and type of edit you need to introduce highly specific? Perhaps you need to introduce a single nucleotide... have little flexibility as to where your sgRNA needs to be located and there may not be a NGG PAM site... and published in peer reviewed journals. If you need to generate a new sgRNA for your experiment, select...

  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...antibody host species and isotypes. Consequently, users need to choose the protein type that is most compatible...those in the nucleus, more stringent buffers may be needed. Since many factors, including salt concentration...strength and pH, affect binding the lysis buffer may need to be adjusted depending on the sample. It is important...be translatable to the endogenous system and will need to be verified using alternative methods.  Finally...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ... modify the protocol to best fit their specific needs. Herein, we’ll give you a rundown of the different...directly conjugated to the reporter, there is no need for a secondary antibody incubation step, saving... the signal. Since the primary antibody does not need to be conjugated, and conjugated secondary antibodies...feel empowered to choose the best ELISA for your needs. Good luck and happy binding! More resources on...
Showing: 281 - 300 of 824 results