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We narrowed to 829 results for: EED

Showing: 281 - 300 of 829 results

  1. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...plasmids and their ability to identify and fulfill a need in terms of helping the scientific community manage... platform, there is the potential for increased speed and efficiency through which knowledge is created...programmatic content as well as help our students succeed whether that be academically or on the employment...great things in a particular area or profession, you need to make sure you have learned and mastered the fundamentals...

  2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...study gene function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs...phenotypes than standard RNAi protocols without the need for mutant genetic backgrounds. The standard RNAi...creating a pair of enzymes that overcome both the speed and toxicity limitations of APEX2 and BioID. In ...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...plasmid service was started based on donations. That seed money was used to build a completely self-supporting...similar model for our Viral Service, we solicited seed money for the new project. New England Biolabs and...ready-to-use viral format. Having identified this need in the community, we approached it in the way Addgene...interesting things going on at Addgene. Sharing speeds science and we are proud to be adding viral vector...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...’ve just made it easier for you to find what you need quickly.  Through the years, we have perfected ...search function. We also quickly realized that we needed to improve more than that: the general “findability... have different strategies for finding what they need, and we wanted to support all of them — searching... our deep understanding of Addgene’s customer’s needs. Categories We created a categorization of our information...

  5. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... and the things you need to include in your manuscript. What information do I need to include in my paper... to anyone who wishes to read it, without them needing a subscription to the journal. An increasing number...reviewers and the editor decide that your manuscript needs to be revised before it can be published, this can...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...transgene-free and vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce...genomic integration Multiple transfections required Need to sequence to verify there’s no genomic integration...-free cells No genomic integration Inefficient Need to verify loss of episome Minicircle Non-integrating...-free cells No genomic integration Inefficient Need to verify loss of minicircle RNA Delivery DNA-Free... difficult to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs...

  8. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...Addgene's resident blog editor, I could take the needed steps to continue where the guide left off - namely...conjugates. And, of course, once you have them, you’ll need to either re-develop or re-optimize your assay for...identified but a smaller or less immunogenic version is needed.  Single-chain fragment variables (scFvs) are ~...will arise in your research or what data you may need to collect. Understanding available alternatives...

  9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...2013). We found that the fluorescent proteins can, indeed, interfere with the correct function of the protein...this was problematic, because the DREADD receptors need to be located on the plasma membrane to be activated...DREADDs. In studies where the fluorescence is not needed, the use of HA and other short epitope tags (Kimple...receptors potently activated by an inert ligand. Proceedings of the National Academy of Sciences 104:5163–...

  10. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...projects. Right now we don’t have to worry about succeeding at our core mission. Plasmid distribution is ...this and recognize it all the time. We love this feedback and are always adding to our “google doc of gratitude...awhile I worry a very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year..., excellent intentions, or incredible drive to succeed.  Thanks to all the Addgenies and congratulations...

  11. To Each HIS Own

    Type
    Blog Post
    ...how many do I need? When designing a plasmid containing a polyhistidine tag, you’ll need to decide whether... binding without eluting the target protein and needs to be empirically determined. In addition, some ...Longer histidine tags are also recommended if you need a very pure sample. Because binding strength is ...

  12. Antibodies 101: Conjugation

    Type
    Blog Post
    ...the research antibodies in your refrigerator often need a little help to be useful. Mainly because, well...available, that never guarantees the antibody you need will be conjugated, or that it will be conjugated...conjugation, it is important to keep in mind that you will need to confirm your antibodies’ performance after the...which can interfere with conjugation reactions. If needed, you can often remove the incompatible agents by...

  13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...stakeholders. However, while most institutions are indeed going ahead with their planned raises, some are...ahead with the raises as they see fit, following feedback from postdocs and faculty (at time of writing,...detrimental effect on the morale of postdocs, and indeed we have been contacted by a number of postdocs ...keep raising salaries and that we are submitting Freedom of Information Act (FOIA) requests for their postdoctoral...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...designed to support new approaches that address the need to train our young scientists for futures both inside... symposium we will discuss where better data is needed to help shape and inform the overall structure ...graduates and postdocs – and from what fields – are needed to adequately supply these demands? Are there other...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

  15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...of the article – via the journal (though you may need to be on a network with an institutional subscription...literature until you have all of the information you need. Reading primary research literature The best way...feasible for every paper you will ever read. So, you need to come up with an organization system that works...works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search. Writing...

  16. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... to see how funds were allocated. Step 3: Get feedback from your peers and advisors Once a rough draft...reach out to peers and collaborators who have succeeded at grant writing to see what tips they found useful...reviewers Reviewers may take 6-12 months to give feedback on the application. Make sure to invest time and...the application process? What types of writing feedback do you find most helpful? Have a great grant writing...

  17. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...your western and your results look… funky? Do you need to start a whole new blot? Never fear: membrane .... Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can be returned...antibodies. If you have already applied antibodies and need to dry the membrane for storage, but plan to reprobe...more strongly or that have saturated the blot may need longer incubation for effective stripping. Some ...

  18. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ... and skew results measuring locomotion. Mice may need to be given some time to adapt to a testing apparatus...stress level, origin (vendor or in-house mouse breeding colony) Environment Olfaction, temperature, ...possible effects. Injection Parameters: The injection needle should be as thin as possible to minimize the damage... damage to the brain tissue. The injection speed has to be slow to allow the viral particles to diffuse...

  19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...drop-down options for you to choose from, while others need to be filled with short-answer text. You can also...partial sequence cells. *Note: If you believe you need to deposit a collection that is greater than 75 ..., S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic, or other. If...appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping ...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...was reported, and we felt that, given the critical need for low-cost, field-based diagnostics, our approach...nights, not unlike most research, but the urgent need for the test did catalyze our resolve to get the...reach out to the greater research community when we needed additional expertise. Perhaps most striking is ...advance our programmable approach to other critical needs and to further develop our rapid prototyping and...
Showing: 281 - 300 of 829 results