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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 638 results for: Erf

Showing: 281 - 300 of 638 results

  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...proteins reported by other research groups that performed BirA*-based affinity purification mass spec experiments...affect protein binding patterns. Hereditary Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized...

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein... from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized...

  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...similar rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing ...advancement in our understanding of how CRISPR will perform in embryos but also shows that many more experiments...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of prime editing ...enhanced prime editing systems improve gene editing performance at therapeutically relevant gene targets and ...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...determines what secondary antibody you should use when performing indirect immunoassays. The Fab regions, on the...before applying them to samples, such as when performing IHC in a sample where your secondary antibody...

  7. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...identification of desired clones.   ...Becomes a powerful cloning tool Molecular biologists first saw the...applications. While these two strains ultimately perform the same function, there is some evidence that ...

  8. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...case, we naively assume that everything will go perfectly and our software simply follows a linear progression... need to be repeated. We also now have a good interface to begin tracking analytics data for lab procedures...

  9. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...concentration, and not all providers are equipped to perform Sanger sequencing from glycerol stocks, so make...Alternatively, you can use DNA-editing software to perform alignments of multiple sequences to visually check...

  10. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...abundance of a specific clone; lineage tracing is performed via barcode sequencing. Using the ClonTracer library...the time to talk with us and for providing this powerful resource to the Addgene community! Carrie Bhang...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    ...transcription factors found in metazoans. The superfamily is comprised of 48 human and 49 murine members...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...

  13. Engaging with science and society at pgEd

    Type
    Blog Post
    ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

  15. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...general questions about job responsibilities, performance evaluations, professional development, and company... research is a deliverable. Find 10 steps to a perfect science talk Be prepared for potential technical...

  16. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...non-target proteins), variability (separate batches can perform differently – happens when the antibody is produced...binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired...

  17. Hot Plasmids February 2024

    Type
    Blog Post
    ...quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have been... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...

  19. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ... a destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick...sequencing and found 98% of the plasmids were perfectly correct. Because they created the assemblies in...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...embryonic genes are individually depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi...RNAi using four-dimensional contrast interference (DIC) microscopy and computer imaging processing. The...
Showing: 281 - 300 of 638 results