We narrowed to 365 results for: FUS
-
TypeBlog Post...Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific...
-
Bioinformatics at Addgene
TypeBlog Post... containerization, but haven’t tried it or are confused by the concept, don’t worry–you are not alone!... -
Hot Plasmids Spring 2024
TypeBlog Post...Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...envelope protein causes cells that it infects to fuse together and form syncytias that cause the cells... -
CRISPR Activation: A Practical Guide
TypeBlog Post...for the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...the microinjection was done as the pronuclei were fusing, you may have several mosaic mice. To figure out... -
TALEN Plasmids and Kits
TypeCollection...Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate...for expression as TALE-LSD1 fusions. It was recently shown that TALE fusions with the histone demethylase...are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors...Takashi Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter... Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids...Visualization [TGV]). pTALYM3 and pTALYM4 contain TALE fused with mClover and mRuby2, respectively, and can be... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...templates for applications that require large gene fusions. The current state-of-the-art in combining the ... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post... composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed... -
Five Popular Model Organisms, Part 2
TypeBlog Post...tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including Xenopus. ... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely, while others ... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All bind specific ... -
Tips for Getting a Faculty Position
TypeBlog Post...answer an expert's question than to leave everyone confused about your research because you waded too deep... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...taking care of that”, because that can indicate confusion around... Ownership: Misunderstandings about who... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...less interference with the oligomeric state of a fusion partner. Therefore, we recommend using miRFPs and... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...available from Addgene. This system uses the dCas9-VP64 fusion commonly used to activate transcription, but with... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...techniques, such as Golgi staining, injection of diffusible labels, or electron microscopy of stained sections... -
CRISPR Plasmids and Resources
TypeCollection... dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information...peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a given... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...actually performed, potentially curtailing future confusion (and hours sifting through paper lab notebooks... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Stanton, A., King, E. M., Ye, S., Tellez, L., Krunnfusz, A., Tavakoli, S., Widrick, J. J., Messemer, K...