We narrowed to 1,039 results for: LAS

....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids ...vectors expressing light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience.... These Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand Tamoxifen is added...neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the...recombinase. After in-utero electroporation, this plasmid mix results in sparse labeling of cortical neurons...labeling of individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However...visualized at the same time from two different plasmids this approach becomes problematic as it reduces...

...single plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...pooled library, which contains a collection of plasmids in a single tube, or a pre-made viral prep library... prepare the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral...library and appropriate packaging and envelope plasmids into HEK cells allows production of lentivirus...amplify the library. This is similar to amplifying a plasmid: transform E. coli with the pooled library DNA,...own protocol that amplifies without disrupting plasmid representation. A good place to start is with a...

...that the plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe... 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent well of HEK... Fig. 1: Expressing bARGSer in E. coli. (a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to ...overexpressed compared to accessory proteins. Excess gvpA plasmid added into the transfection mixture in HEK293T ...mammalian species to produce gas vesicles. New mARG plasmids Our new mARGs come as a set of two PiggyBac integratable...genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient transfection... into the genome by cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines...

...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...to July 27, 2020. Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents...

... After all, students build circuits in physics class to learn about electronics or mix chemicals together...everyone safe. None of this is conducive to a classroom setting, especially for a school with a limited... activities making their way into high school classrooms, like gel electrophoresis to separate DNA or ...translation. Unfortunately, having live cells in a classroom presents equipment needs, such as shakers and ...FD-CF pellets are easy for students to use in the classroom because the students simply need to add water ...developed the BioBits kit to be used in high school classrooms, but it could be widely adapted and used in a...transition your undergraduate students from the classroom to the lab. You could give your undergrads hands-on...

...Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for...microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with fluorescent... To make it easier for you to find plasmids for your work, plasmids in the Microbiology Collection are... a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...us at [email protected]. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene...develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org/deposit ...

...transfection of single plasmids vs pooled plasmids; for multiple edits, single plasmids are generally more...transfected on a single plasmid. This is versus 2.4% of cells transfected with a pool of plasmids that contained...sites. This eliminates bulk from crRNA expression plasmids resulting in smaller, more streamlined vectors...contained one crRNA per plasmid plus a Cpf1 expression vector.  See the graph in figure 2 for a comparison of...of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes...your research? Check out the Cpf1 multiplexing plasmids available from Addgene. If you have any questions.... Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3), 759-771. PubMed...

... expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression vector... express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing... (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based ...been engineered to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded...lentiviruses. This means that if you received lentiCas9-blast lentivirus from Addgene, you could use it in conjunction...own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...

... awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...Learn about the Addgene, Seeding Labs Plasmid Grant Read about Plasmid Grant Recipient, Gladys Kaba Resources... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s Newsletter and recently ...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian... Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher... recent recipient of the Addgene, Seeding Labs Plasmid grant and is working on a variety of projects in...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular and Molecular Bioengineering...Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools... drug inducible Cas9 plasmids E. coli genome-wide inhibition library In the last edition of “What’s New...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...vector sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli and human...expression. Read the paper in Nature Find the CasX plasmids Drug inducible transcriptional activation and...estrogen receptor. This protein fusion stays in the cytoplasm, but with the addition of 4-hydroxytamoxifen, ...

...Turquoise (CeNL; Plasmid #85199), Yellow (YeNL; Plasmid #85201), Orange (OeNL; plasmid #85202) and Red ... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to ...kDa. An example is the CRISPaint-NLuc construct (Plasmid #67178) for use in the system outlined in Schmid-Burgk...CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids for Antares or firefly luciferase into mouse livers...Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions of.... This construct is available through Addgene (Plasmid #83926). Live-Cell Voltage Sensor  Ingaki and ...

... involving two-plasmid or single-plasmid systems (Vo et al., 2020). In the two-plasmid system, an effector...group’s initial report of VchINT, three separate plasmids and multiple promoters were used to express the...effector plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter...promoter, and a donor plasmid (pDonor) encodes the mini-transposon. pEffector and pDonor can also be combined...combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller cargos, and ...and the ability to easily cure cells of the plasmid after integration. Figure 2: (A) Streamlined ...insertions with Type I-F INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow...

...Best Way to Elute and Store Your Plasmid DNA? Selecting Your Plasmid Purification Kit Resources on Addgene.org...You have just purified your plasmid and are ready to move on to your downstream application — but wait...wait! Do you know how much plasmid DNA you have or how pure your sample really is?  To assess the concentration...concentration and purity of nucleic acids, such as plasmids, the go-to method is to use spectrophotometry....Protein contaminants Whether you are purifying plasmid DNA from bacteria or cleaning up a PCR product,... 230 nm is guanidine thiocyanate (used in many plasmid purification kits), which can dramatically reduce...assay or running an agarose gel. In the case of plasmids, the use of RNase treatment to degrade bacterial...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...284 . https://doi.org/10.1038/nature24049 Find plasmids from this paper at Addgene. Amrani N, Gao XD,... https://doi.org/10.1126/science.aaq0180 Find plasmids from this paper at Addgene. Kim D, Kim J, Hur...863–868 . https://doi.org/10.1038/nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo ...8: . https://doi.org/10.1038/ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver...485 . https://doi.org/10.1038/nature14592 Find plasmids from this publication at Addgene. Kleinstiver...869–874 . https://doi.org/10.1038/nbt.3620 Find plasmids from this publication at Addgene. Hu JH, Miller...

... scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our Plasmids 101 eBook!...as easy as obtaining a plasmid with the gene of interest (search Addgene plasmids by gene here), designing...fragments (containing regions of homology) cut from plasmids via restriction enzyme digestion, and used directly...as simple as combining your favorite linearized plasmid with a synthetic oligo that contains a region of... desired edits to the target region. Assembled plasmids can be directly transformed into E.coli for plating...Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional Resources on Addgene....Find Validated gRNAs Browse All Plasmids ...

...integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details on how to...advantage for researchers looking to generate long-lasting changes in cells. However, for some applications...lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long...this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically...production of lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus...into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral...

...e.g., sister chromatid, plasmid, etc.), and consists of three pathways: classical double-strand break repair...covered in detail here. Classical double-strand break repair (DSBR) In the classical DSBR pathway, the 3’...disrupted in the donor. Choosing a type of donor: plasmid, oligo, double or single stranded? The size of ...longer than 200 bases can be difficult, thus dsDNA plasmids are generally preferred for large insertions such...fluorescent proteins or selection cassettes. This class of template should have homology arms between 500...500-1000 bps. HDR efficiency with circular plasmid templates is generally low; to increase the frequency...edits, researchers have designed self-cleaving plasmids that liberate the targeting region from the vector...

...There are two IgA subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies...There are a lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list...switch isotypes? What is an isotype? An isotype is a class of antibody that’s determined by its heavy-chain...and α. Some isotypes have different versions or subclasses that have minor differences in their heavy chain...the hinge region in IgM antibodies. IgM has no subclasses and is found mostly in blood. IgD While IgD is...monomer structure with a valency of two and has no subclasses. IgG IgG is the most abundant isotype in blood...monomer with a valency of two. There are four IgG subclasses, numbered based on their abundance in blood: ...

...Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression systems...indel generation for two PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing...3 and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly higher mutation...mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How many loci can...gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted genomic sites separated...common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. al are specifically designed for....1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication at Addgene Other Techniques...