Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 765 results for: Mos

Showing: 281 - 300 of 765 results

  1. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...experiment. You may find yourself defaulting to the most popular or familiar options without even realizing...directly impact the way you can utilize these dyes. The most-used fluorescent dye is fluorescein isothiocyanate...matched with the experimental setup and objectives. Most systems can only excite and read a set number of...such as superior brightness, photostability, and most importantly, a photoswitching ability. This photoswitching...

  2. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...that is more neutral, causing PA-GFP to display almost no fluorescence when illuminated with 488nm light...emission of green light is more phototoxic to cells, most scientists working on in vivo imaging models perfer...photoactivatable fluorescent proteins Some of the most important applications for PA-FPs involve enhancing...0.5-1% osmium tetroxide). These conditions destroy most PA-FPs, but, in 2015, the Looger Lab designed 2 ...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...consider the donor first where labeling specificity is most important. Donor labeling and the need for specificity...changes due to this bulky insertion are inevitable. Most FP fusions for FRET studies are at the N- or C-terminus...is required and thus far, this method has proven most effective only in a few cell types (E. coli and ... in chemical warfare [Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...various tools and components necessary to run it? And, most importantly, what are the methodological and experimental...one: Deciding how you want to use optogenetics The most important question to consider is what exactly do...neuronal promoters such as synapsin or camKIIα drive most of the constructs. As an alternative, some constructs...of interest (as shown in the figure below). Since most of the precision of optogenetics is dependent on...

  5. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...year of first reagent sent, year with most reagents sent, and the most recent full calendar year When the... information can provide valuable insight to the most requested plasmid, the number of requested plasmids...

  6. Editor's Choice, July 2016

    Type
    Blog Post
    ...Editor's Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more...excitement surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an...and similar on-target editing when compared to the most commonly used Cas9 variant, SpCas9. These properties...

  7. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    ...Developmental Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In...monoclonal antibodies obtained from serum.  Our most popular monoclonal antibodies include those directed...More Blog Posts about Scientific Sharing: The 10 Most Distributed Plasmid Technologies in Addgene's First...

  8. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... of all citations in 1995 to 47% in 2013 – yes, almost half.   The effect of online access on scientific...interesting aside, Nature recently reported that the most cited work in history is a 1951 description of an...Prize-winning research typically weren’t among the most cited. And, as scientific discourse has increasingly...

  10. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ... Protein purification can be one of the most stressful lab activities. Working with proteins requires...proteins are overexpressed from a plasmid construct, most often in special E. coli strains designed for protein...few different vectors to see which gives you the most functional protein. The pCri system, recently deposited...

  11. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...coding, labels, and post-its to make finding your most used protocols & references easy to find It’s happened...know where to start your search. Then identify the most frequently used pages in your notebooks and go wild..., or references that your labmates ask to borrow most frequently. Copy these into an electronic file sharing...

  12. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...associated with plasmid-based overexpression. Tim Formosa, at the University of Utah, has built a complete...terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning...

  13. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...my interest was renewed in part due to FOMO  but mostly because of this article: “A systematic identification...2017. 1. Among scientists, historians have the most Twitter users. Biologists rank #8. Figure 1: Highly...than other disciplines. The majority of tweets for most disciplines contain scientific domains between 0...

  14. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...Overview of the parts of CRISPR. The bacterial chromosome encodes a tracrRNA (in some systems including...are called Cas, short for “CRISPR-associated.” The most famous of these proteins is Cas9 nuclease, but there...DNA CRISPR array: The section of the bacterial chromosome that stores a genetic record of previously encountered...

  15. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... Unfortunately, unless they’ve worked in a lab, most people don’t get to experience this very human side...the stop motion format for our videos as it is the most approachable for LEGOs. Stop motion consists of ...takes an entire week of our free time. After taking almost a thousand pictures, my husband Enzo will spend...

  16. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... a scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors...inhibited in sharing concerns. Ask alumni of the lab. Most labs have websites listing alumi and it is easy ...ResearchGate and LinkedIn to find past lab members. Most scientists love to talk about their past lab experiences...

  18. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...non-academic jobs as “alternative” anymore.   Since most scientists start out in academia, it is especially...waiting Work is concentrated to business hours (mostly), but can often achieve more structured time management...and what parts of being a scientist you enjoy the most. Having a better idea of where you want to go will...

  19. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... development as your mentor. How do you make the most of this new relationship? Consider adding formality... a mentoring relationship really productive, the most important step you can take is to set goals to work...teaching to help a Mentee reach goals. One of the most important contributions made by a Mentor is to help...

  20. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...contain several important elements, but among the most important are the inverted terminal repeats (ITRs...modifications and types  Since ITRs are one of the most important elements of AAV and rAAV vectors, modifications...T-shaped hairpin loop sequences (Wilmott et al. 2019). Most ITR sequences are from AAV2 because they demonstrate...
Showing: 281 - 300 of 765 results