We narrowed to 928 results for: Mpl

...some data, etc.  The firestorm that followed implementation of the policy comprised of numerous objections...raw data requires huge amounts of storage. For example, mouse behavioral geneticists may track the motion...although it might be argued various authors have simply chosen to submit elsewhere). The majority of the...numbers of figures such that many have multiple, complex panels. PLOS, itself, has not finalized its definition... paper are in scope. For images, the number of examples supporting the published material is a factor ...types? And how long should data be archived? Simplifying data access Some of the above mentioned issues...collections and interpretation of data, publishing complete data, rather than just a single projection of ...

... relatively simple, changed the course of scientific research. What was once a complicated process of ...and requesting scientists, all of the Addgene employees, the Addgene board members, vendors, Addgene supporters...prototype of an interactive game that Addgene employees created and designed for the Hall of Human Life...playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you might have... on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as plenty of delicious...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey...

...then following transduction, the partial genomes complement each other to restore the full-expression cassette...strategy for increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs ...co-transduced with multiple copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping...outperform single oversized AAVs (Hirsch et al, 2013). Examples of use: Lai et al., 2005 used a trans-splicing... the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008 used a hybrid split...homologous recombination approach delivers large donor templates using two sequential homology directed repairs... the gRNA target site that mediated the donor template’s integration so that the site is reconstituted...

... by a ribonuclease called Dicer. In a great simplification of the process, Dicer chops off the loop of...create pri-miRNA, which is then processed by a complex called Drosha to pre-miRNA. miRNA plasmids are ...RNA duplexes bind to the RNA-induced silencing complex (RISC). After binding to RISC, the RNA duplex is...t always cleave the mRNA — the bound miRISC can simply block translation machinery. The composition of...typically range from 19–22 nucleotides and are 100% complementary to their target sequence. However, you can’t... day. You need to include both sides of the complementary RNA duplex and the hairpin loop sequence (Figure...action, and degradation of RNA-induced silencing complex. Molecular Cell, 82(1), 30–43. https://doi.org/...

...to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent protein ...endogenous fluorescent co-factors can be used. Examples include the LOV domains that bind flavin (Buckley... pKa, which is around 6.0 for GFP. This number implies that at pH 6 only 50% of the available green fluorescent...under acidic conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP (pKa =...diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence ...specifically and with high affinity to fluorophores. Examples are the FlAsH tag, the His tag and several others... proteins are also a better choice for thicker samples. Another solution to avoid autofluorescence is ...

...Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR..., and the spacer sequence is used to target complementary DNA sequences. In laboratory settings, an sgRNA...whose sequence is not checked. Assuming full complementarity, its sequence matches the guide sequence. PAM...into a single strand that folds back on itself, simplifying the CRISPR-Cas9 system for laboratory applications...either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide and target...post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR ...

...GPCRs (Takeda S et al., 2002) and many downstream complexes involved, it can be difficult for scientists to...in sight (Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, ...collectively known as a heterotrimeric G protein. This complex converts a signal received by a GPCR on the cell...is being able to identify specific transducer complexes that are activated in response to a ligand binding...inactive, these subunits are found in a trimeric complex bound to the intracellular surface of the GPCR....Following activation in response to the ligand, the complex will disassociate, resulting in a decreased GFP2...screening across the transducerome, to gain a more complete understanding of a GPCR mediated response.  Acknowledgements...

...communicating science to laypeople.  Laypeople are simply non-experts in the field of science you are communicating...use-case (definition) in a specific field.  For example, while “PCR test” has recently transitioned from...PCR test” to mean “a test that uses a biological sample to look for presence of a virus.” A scientist would...sequence that uses trprimers and enzymes that will amplify a sequence only if present.” You can see how you...the possibilities are endless.    Activity Example audience  Talking about your career as a scientist... podcast about genetics using dog breeds as an example adults 45-55 yrs old who breed or are interested...online patient/caregiver communities  Table 1: Examples of defining your audience for science communication...

...will result in more engaged, successful employees who can accomplish great things.   To read more on this...time for that and you won’t be as productive. For example, the many resources available on the Addgene website...enough to do…you are not delegating enough. For example, if we need to develop a new plasmid collection...duplicated. With each component of the project completed by an individual who is a local expert in that...result is high quality and the entire process is completed more efficiently. Listen to our podcast interview...desired outcomes. Concern yourself with what is accomplished, rather than detailing how the work should be...

...minipreps for small batches of samples. As soon as that process is complete, QC scientists prepare Sanger...mutation.  Once the samples arrive, each group of plates will have select samples prepared and screened...available. Plasmids are continually added as they complete QC and you can find all available plasmids listed...screened by NGS to spot check the samples, the rest will be put into a status we call “Archived.” This status...for all several thousand plasmids at once. Any samples that are requested by scientists, will immediately...

...achievement, the complexity of the device increases, as does the price. With this increased complexity comes the...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...teleporter," since a part developed in Germany, for example, can also be printed quickly in Madagascar. Platforms...a move towards more open research. The EU, for example, is calling for the opening of sources within the...hardware.    Conclusion Addgene is a great example of how sharing of data and molecular tools can ...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and...

...Chemical agents, temperature, and light are all examples of factors that can lead to the induction of a... these three types of inducible promoters, and examples of each type. Many of these promoter systems are...promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...represses pBad, decreasing promoter leakiness.   Other examples of chemically induced promoters include positive...in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated...temperatures but can be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90...

... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...differences in these approaches can be found. As an example, say you are considering either a cellular screen...libraries targeting all known genes. This is a perfect example of an unbiased, reverse screen – all known genes...possessing a specific mutation or phenotype. For example, a drug screen may be performed on a cell line ...patients may respond to drug treatment. Another example is using a mouse model of a particular disease ...previously defined disease phenotype. These screens employ the same downstream manipulations as a forward ...that do not affect the function of interest. An example where a selection would work is if you are trying...

... For example, setting up your pipette tips to match your plate layout can help you track samples as you...Identifying the Issue You can’t fix an experiment by simply running it unchanged over and over again, and the...change variables to fix a failed experiment.  For example, I used to constantly underestimate time needed...was not trying even harder to start earlier, but simply moving all my prep work to the day before, which... and make a checkmark next to each step as you complete it. Yes, this can be incredibly tedious, but it...remember what's been added and what hasn't. Developing simple habits like these can consistently reduce errors...

...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...manager works with software developers to help implement solutions for the stakeholders. Finally, software...all fits in with science. Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software...backgrounds, most did not. It was my job to translate complex lab operations workflows into a format the developers... to solve is clear, but the solution is more complicated. You’ll need to be creative to be able to work...working with data, working with people, working with complex processes Arm yourself with knowledge of software...catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss which front-...

...results? Or considered why DpnI will degrade your template DNA from a PCR reaction but not the newly synthesized... Methylases involved with these processes (for example Dam and Dcm methylases) are independent from the...conversely, activating enzymes such as DpnI. For example, cleavage by XbaI may be blocked due to methylation...no methyltransferases in the PCR reaction). The template plasmid, on the other hand, should be derived ...with DpnI, only the non-mutated and methylated template is destroyed leaving behind a pool of mutated ...those sites will be methylated. Using the same example plasmid as above (which has the XbaI site blocked...methylation by altering your choice of bacteria. For example, if you must use a restriction site that will be...

...this complex holds a pair of DNA ends together, forming a paired end complex The paired end complex then...repair, as there is no requirement for a repair template (sister chromatid or homologue) or extensive DNA...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does... factors can also influence NHEJ activity. For example, repressing RecA and overexpressing the NHEJ machinery...NHEJ-dependent repair (“pop-in” insertion) provided a template containing compatible overhangs is available. ...

...predicted structure of your antibody-antigen complex as a template. This structural framework then guides the...equipment on a lab bench. The best part? Less complicated math. Bioengineering is a field that has been...methods. (A) Basic structure of an antibody. (B) Examples of engineered antibodies, including chimerization...directed evolution Directed evolution is a method employed to rapidly change a protein towards a specific...achieve whatever goal you’re aiming for. When you complete each cycle, your best-performing mutant is usually...different primers. While error-prone PCR is usually employed to mutate whole antibody sequences, degenerate...specific functionality you’re looking for. For example, if you were aiming to increase an antibody’s binding...

...Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo use at the University...hippocampus and cortex) within the mouse brain (4). An example from the cortex is outlined in the table below....is possible to use AAV constructs with self-complementing structure (scAAV) (7). As single stranded DNA...mapping of circuits In our lab, we use AAV-DREADD complexes to study neural function. Often the reporter proteins... proteins associated with these complexes are so robustly expressed that anatomical assessments can be...be easily performed too. While I implied that axoplasmic transport of AAVs is a potential problem in many... E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors...

...presentation, lost in a sea of data, my mind went completely blank. After several seconds of truly panicked...honest, if my paper hadn’t been ridiculously more complex than the one before it, I don’t think I would have...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets... panicked state, only option one would let me accomplish my goal.   As a scientist, public speaking isn't...dumpster noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes... in its most difficult form. You’re presenting complex data, to a group of peers who are there to provide...