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We narrowed to 550 results for: NOL

Showing: 281 - 300 of 550 results

  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...control protein expression for experimental, biotechnological, and medical applications. Researches can ...desired protein. Despite being a rather nascent technology, plant cells have been used to express a wide...

  5. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

  6. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips (USA...where it immediately spreads out to form a firm monolayer. The cell clones - easily identified from the ...

  11. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...anchoring strategy for expansion microscopy. Nat Biotechnol 1–12. https://doi.org/10.1038/s41587-022-01546...standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038/nbt.3625  ...

  12. DNA Purification Without a Kit

    Type
    Blog Post
    ...RNase to remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins...of four times before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms...

  13. MXS Chaining

    Type
    Blog Post
    ...proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent protein...High Throughput Cloning Strategies Method Technology Pros Cons References Golden Gate Type II restriction...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic...research is being done to further improve the technology: engineering of new capsid variants, designing...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts... many other uses currently exist, and new GFP technology is constantly being developed! Fusion tagging...

  18. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ..., Methylophilus methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...

  19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

  20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...
Showing: 281 - 300 of 550 results