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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 551 results for: NOL

Showing: 281 - 300 of 551 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    ...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids...-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID: 27347757. Find plasmids...

  5. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...control protein expression for experimental, biotechnological, and medical applications. Researches can ...desired protein. Despite being a rather nascent technology, plant cells have been used to express a wide...

  6. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips (USA...where it immediately spreads out to form a firm monolayer. The cell clones - easily identified from the ...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic...research is being done to further improve the technology: engineering of new capsid variants, designing...

  15. MXS Chaining

    Type
    Blog Post
    ...proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent protein...High Throughput Cloning Strategies Method Technology Pros Cons References Golden Gate Type II restriction...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...anchoring strategy for expansion microscopy. Nat Biotechnol 1–12. https://doi.org/10.1038/s41587-022-01546...standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038/nbt.3625  ...

  17. DNA Purification Without a Kit

    Type
    Blog Post
    ...RNase to remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins...of four times before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms...

  18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts... many other uses currently exist, and new GFP technology is constantly being developed! Fusion tagging...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...also be used with the most cutting-edge imaging technologies such as array tomography, super-resolution STORM...W. Davidson. "Advances in fluorescent protein technology." Journal of cell science 120.24 (2007): 4247...
Showing: 281 - 300 of 551 results