We narrowed to 423 results for: NUC

1. Hot Plasmids and Viral Preps - July 2021

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   ...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...

2. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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   ... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...

3. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   ...researchers used the HA frankenbody to successfully track nuclei in a developing zebrafish embryo over an 80 minute...

4. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ... find the ACE reporter plasmid! Martin et al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ...

5. Summer SciComm Series: Master of SciComm

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   ... nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics researcher on...

6. Hot Plasmids and Viral Preps - September 2021

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   ...disease-causing point mutations. They allow single-nucleotide modifications to edit DNA without requiring a...

7. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   ...Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...

8. 10 Great Guest Posts We're Thankful For

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   ...chemical fluorophores for your next experiment. Nucleic acid based machines Recombinase-based State Machines...

9. Using Addgene's New Viral Service for Your Research

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   ...with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors...

10. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    ...transferred between cells. At the time, these extranuclear agents of heredity were thought of as everything...

11. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ...use the serotonin receptor to study how single nucleotide polymorphisms present in the human population...

12. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    ...intended parents is “rehoused” in a separate, enucleated zygote with healthy mitochondria, with the intent...

13. The Importance of High Titer for AAV Transductions

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    ...through each part of the cell on their way to the nucleus where their gene cargoes can be expressed. The...

14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    ...surface, in the cytosol, in mitochondria, or in cell nuclei. The two photosensitizers had similar rates of ...

15. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic...

16. With Our New Viral Service, We're Taking CRISPR Further!

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    ...it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent problem...

17. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...Microinjection requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation on the...

18. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have...

19. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...Sanger sequencing determines the precise order of nucleotides within a DNA molecule, in this case a plasmid...

20. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...DSB is repaired by NHEJ which depending on the nucleotides inserted or deleted, can result in an in-frame...