Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 568 results for: PAC;

Showing: 281 - 300 of 568 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...the protein would be helpful to know for viral packaging limitations. Maybe you want to read literature...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...with some of our most popular biosensors already packaged and ready to deliver. Ready to give biosensors...AAV as your vehicle, we’ve got you covered with packaging plasmids! References and Resources References...

  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...tricky to find: each of the factors can negatively impact both translation and fidelity of translation, leading.... Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan...

  4. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral applications...just one at a time. Many of these libraries are packaged and delivered with viral vectors instead of a ...

  5. Viral Production

    Type
    Collection
    ... Viral Vector Packaging Service Viral Production Viral Production Jump...cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell culture...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ...distributed milestone! We were thrilled to see the impact our repository was having on the scientific community...expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...

  10. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientists know the results, your work won’t have any impact! There are lots of ways scientists communicate...efforts, inspire future research, and increase their impact. Open access and transparency Another major benefit...

  11. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...amazing affinity reagents we just didn't have the space to give proper credit to. Thankfully, as Addgene's..., or be able to access an epitope folded into a space that would size-exclude an antibody. In imaging ...

  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...Future of Research Learn How Notions of Scientific Impact Are Evoloving Check Out Extracurricular Activities...

  13. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...the many freely-available DNA-editing software packages to assemble your expected full plasmid sequences...when designing a sequencing primer, you need some space between your primer binding site and the first feature...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified pFUS array vectors...

  16. Engaging with science and society at pgEd

    Type
    Blog Post
    ...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....

  17. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid website...

  19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...breed. Some problems with transgenic mice that can impact reproduction include embryonic lethality, a short...should be tested in cell lines to determine how it impacts the expression level of the transgene. In existing...

  20. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of histone-modifying...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine...
Showing: 281 - 300 of 568 results