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  2. Clear and Accessible Writing

    Type
    Blog Post
    ...the use of alt text to describe images in online spaces. Alt text is used by screen readers to provide ...professional development workshop, “Make a Greater Impact Using Clear and Accessible Scientific Writing.”...

  3. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...the protein would be helpful to know for viral packaging limitations. Maybe you want to read literature...

  4. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...the work you've done. If you want to maximize the impact of your communication, you will also have to explain...communicate with non-scientists, to establish broader impacts for their work in education and policy and to draw...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...amazing affinity reagents we just didn't have the space to give proper credit to. Thankfully, as Addgene's..., or be able to access an epitope folded into a space that would size-exclude an antibody. In imaging ...

  8. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  10. Addgene: The First Twenty Years

    Type
    Blog Post
    ...distributed milestone! We were thrilled to see the impact our repository was having on the scientific community...expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...

  11. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientists know the results, your work won’t have any impact! There are lots of ways scientists communicate...efforts, inspire future research, and increase their impact. Open Access and Transparency Another major benefit...

  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...Future of Research Learn How Notions of Scientific Impact Are Evoloving Check Out Extracurricular Activities...

  13. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...the many freely-available DNA-editing software packages to assemble your expected full plasmid sequences...when designing a sequencing primer, you need some space between your primer binding site and the first feature...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of histone-modifying...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine...

  15. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ...the calcium sensors described are also already packaged into viral vectors and ready to go. Pro tip: Vectors...

  16. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection....   Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.  ...

  18. Engaging with science and society at pgEd

    Type
    Blog Post
    ...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....

  19. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid website...
Showing: 281 - 300 of 555 results