Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 395 results for: POR C

Showing: 281 - 300 of 395 results

  1. All in a Twist: dsRNA

    Type
    Blog Post
    ...and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with... how these structures are generated, why it’s important for cells to sense their formation, and how clinicians...in turn lead to genome instability. Thus, it’s important to strike a balance between productive levels ...be delivered or anticipating this response are important considerations when using these reagents. RNA...recognized by RNase H which hydrolyzes the RNA portion, allowing the DNA strands to re-pair. The two dominating...stranded isn’t just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease...

  2. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski...114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding... from the Allen Institute for Cell Science data portal (Link opens in a new window) , including: data ...

  3. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...Methods Mol Biol, 2018. PubMed PMID: 29043675.Grefen C and MR Blatt. Biotechniques 2012. PubMed PMID: 23066669...2017). However, Acr inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument...versatility opens up the potential for spatial and temporal control in many genetic targeting and editing ...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ...response to light, offers a way to spatially and temporally control cellular processes in vivo. Many photoactive...

  4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...proven in (A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...potential conflicts that waste money and time. Most importantly, “the company does not become real until someone...Synthetic Biology? Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup...

  5. Hot Plasmids - February 2022

    Type
    Blog Post
    ...binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling various... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial matrix...pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP...N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant resistance... to report mitochondrial turnover Mitochondrial health, transport and turnover are important features...genes, reporters, or other markers into a target genome. They did this by selecting the important elements...usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer from Yan’s ...Gottlieb’s lab has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to...

  8. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...photosensitizer fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite...

  10. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

  11. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...python, R, perl, and ruby // This is a comment in C++ and Java Command line history: Tracking command...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal dynamics in response...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional...gene. Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters are delivered...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from ...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ...Cre-dependent reporter expression, the potential for background expression of the reporter will be greatly...shown in Figure 2, the cassette should contain a reporter DNA sequence (such as GFP) in the sense orientation...the two pairs of target sites. By flanking the reporter with one loxP site and one lox511 site in the ...

  14. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...antibody structure with important features labeled. V = Variable domain. L = Light chain. C = Constant domain...from another portion. For example, Fab fragments are molecules composed of just the Fab portion of the antibody...between the target and reporter. They have fewer non-specific binding opportunities due to the lack of an...region is the “tail” of the antibody and comprises portions of the constant regions of the two heavy chains...used in vivo.     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

  15. AAVED

    Type
    Collection
    ... Deverman BE, Matho KS, Cetin A, Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J...Sponsor We are grateful to The Kavli Foundation for supporting this meeting. The goal of this year’s workshop...

  16. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses in the left and right...changes. In addition to supporting in-person conference attendance, the award now supports virtual conference...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...hydropersulfides modulate protein activity and have important roles during oxidative stress. To monitor this...

  17. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...manipulation. Common inbred strains include C57BL/6, Balb/c, and 129. Conversely, outbred strains were originally...gene incorporated into their genome. While transgenic mice have the new gene construct incorporated randomly...embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome. In ...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides...

  18. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...their monovalent nature. They are stable up to 80°C and can refold correctly after denaturing.  Designed...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  19. Antibodies 101: Conjugation

    Type
    Blog Post
    ...doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...
Showing: 281 - 300 of 395 results