We narrowed to 400 results for: POR C

...arrays (e.g. for spacers A, B, and C, you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central... Find plasmids from this paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for ...

...to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based...milestone. Viral service supporters We would like to thank the companies who supported this project with funding...and be ready to provide extensive logistics support for export and shipping on dry ice. The Business and...seed money was used to build a completely self-supporting service so that Addgene is not dependent on grants... in accelerating the Viral Service. With their support we are able to provide faster access to a larger...number of viral preps. Visit our Sponsors and Supporters Page Thank you to all of our generous, sharing...Addgene repository. Without these scientists and the support of their technology transfer offices, Addgene would...

...this may be due to different lipid compositions of C. elegans membranes, which might beneficially influence...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ...cells. These changes were larger than previously reported for other organisms, and the Gottschalk group ...

...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht...about the process that was used to create this important collection.  Creating the Plasmids To begin constructing...antibody (R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue...sequences available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for...why choosing the right secondary is of primary importance. PLoS One. 2012;7(6):e38313. doi: 10.1371/journal.pone...

... Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...promoter is right for me? Here’s a list of some important variables to consider when choosing an inducible...’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky....

...depositing can be grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks...drop-down menu to indicate whether the tag is on the N or C terminus and whether it was present in the backbone...be entirely verified by sequencing. Upload any support files that will make it easier for scientists to...

...    References and Resources References Drummond, C., & Fischhoff, B. (2017). Individuals with greater...how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight...to all the optional sexual health sessions and reported the intel back to my shyer classmates. For my ...the community During my PhD, I’ve also found opportunities for more hands-on science communication. Between...

... tracrRNA and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound to the Cas9... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

...synthetase subunit E. coli p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA...(CePheRS) C.elegans p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA...tRNA is incorporated to the growing peptide chain. To expand the genetic code and incorporate a ncAA into...by reassigning specific codons to enable the incorporation of amino acids beyond the 20 standard ones, ... cell, the ncAA provided in the media can be incorporated using the existing protein translation machinery... tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This plasmid often carries multiple tRNA...target plasmids in genome code expansion for the incorporation of non-canonical amino acids (ncAA) in a protein...

... Since the bacteria are subsequently grown at 37 °C, the recombineering plasmid is not maintained. Recombination...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

...ti2learning.org. References 1. Dieterich, Daniela C., et al. "In situ visualization and dynamics of newly...designated work areas and come with health risks. Importantly, with this method it is not possible to follow...azidohomoalanine (AHA). In the technique, AHA is incorporated into newly synthesized proteins instead of methionine...endogenous protein of interest that also had incorporated the AHA tag after pulse labeling of a determined...FUNCAT-PLA provides both quantitative and spatio-temporal information about the turnover of specific endogenous..."Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains." Proceedings...Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings of the National Academy...

...and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with... how these structures are generated, why it’s important for cells to sense their formation, and how clinicians...in turn lead to genome instability. Thus, it’s important to strike a balance between productive levels ...be delivered or anticipating this response are important considerations when using these reagents. RNA...recognized by RNase H which hydrolyzes the RNA portion, allowing the DNA strands to re-pair. The two dominating...stranded isn’t just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease...

...Methods Mol Biol, 2018. PubMed PMID: 29043675.Grefen C and MR Blatt. Biotechniques 2012. PubMed PMID: 23066669...2017). However, Acr inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument...versatility opens up the potential for spatial and temporal control in many genetic targeting and editing ...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ...response to light, offers a way to spatially and temporally control cellular processes in vivo. Many photoactive...

...proven in (A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...potential conflicts that waste money and time. Most importantly, “the company does not become real until someone...Synthetic Biology? Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup...

...binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling various... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

...specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

...targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial matrix...pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP...N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant resistance... to report mitochondrial turnover Mitochondrial health, transport and turnover are important features...genes, reporters, or other markers into a target genome. They did this by selecting the important elements...usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer from Yan’s ...Gottlieb’s lab has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to...

...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...photosensitizer fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite...

...sequence similarity to previously identified Cas13a-c orthologs, but they do include HEPN nuclease domains...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...