... Deverman BE, Matho KS, Cetin A, Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J...Sponsor We are grateful to The Kavli Foundation for supporting this meeting. The goal of this year’s workshop...

...binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling various... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

...specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

...targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial matrix...pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP...N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant resistance... to report mitochondrial turnover Mitochondrial health, transport and turnover are important features...genes, reporters, or other markers into a target genome. They did this by selecting the important elements...usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer from Yan’s ...Gottlieb’s lab has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to...

...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...photosensitizer fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite...

...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

...sequence similarity to previously identified Cas13a-c orthologs, but they do include HEPN nuclease domains...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

...python, R, perl, and ruby // This is a comment in C++ and Java Command line history: Tracking command...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

...combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal dynamics in response...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

...References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional...gene. Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters are delivered...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from ...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ...Cre-dependent reporter expression, the potential for background expression of the reporter will be greatly...shown in Figure 2, the cassette should contain a reporter DNA sequence (such as GFP) in the sense orientation...the two pairs of target sites. By flanking the reporter with one loxP site and one lox511 site in the ...

...antibody structure with important features labeled. V = Variable domain. L = Light chain. C = Constant domain...from another portion. For example, Fab fragments are molecules composed of just the Fab portion of the antibody...between the target and reporter. They have fewer non-specific binding opportunities due to the lack of an...region is the “tail” of the antibody and comprises portions of the constant regions of the two heavy chains...used in vivo.     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

...stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses in the left and right...changes. In addition to supporting in-person conference attendance, the award now supports virtual conference...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...hydropersulfides modulate protein activity and have important roles during oxidative stress. To monitor this...

...manipulation. Common inbred strains include C57BL/6, Balb/c, and 129. Conversely, outbred strains were originally...gene incorporated into their genome. While transgenic mice have the new gene construct incorporated randomly...embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome. In ...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides...

...their monovalent nature. They are stable up to 80°C and can refold correctly after denaturing.  Designed...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

...doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

...References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

... AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki...nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stemloop...

...islands (regions of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to ... versions of this reporter to target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27...The K9 reporter uses the HP1 chromodomain to recognise lysine 9 methylation, while the K27 reporter uses... methylation in living cells. Essentially this reporter construct consists of one substrate (the histone-derived...-K27 histone methylation reporter) positions on histone H3, which are known to be involved in repression...methylation of these sites was controlled, the reporter consistently showed significantly different FRET...states. Thus, this system is a reliable tool in reporting methylation status of these sites. This new technology...

... PubMed Central PMCID: PMC3622210. Schek N, Cooke C, Alwine JC. Definition of the upstream efficiency ...expression levels, the terminator also plays an important role in RNA processing and contributes to variability...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...degradation, and is integral to the nuclear export and translation processes as well.   Figure 2... non-poly(A) transcript-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use...the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1 Gag expression ...

...procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which can be used to confirm...Professor at Boston College. National reports stress the importance of providing authentic research experiences... is particularly interested in strategies to incorporate research into undergraduate courses and in communicating...