... new prime editors, PE6a–g, that overcome some current prime limitations and enable impressive editing...optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits with similar...similar or better efficiency than PEmax, the current state-of-the-art. PE6b was very efficient for short...types. DIRECTED is compatible with fusogens from different viral families and can be used to target multiple... enzymes, TurboID and APEX2, are localized to different cellular compartments. They showed APEX2 can use...uses sequential labeling by TurboID and APEX2 at different cellular locations. C) Enrichment of dual-labeled...stably or inducibly express tagged iPAK4 from different promoters, specific time points appear as fluorescent...

...these tools can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...93,491 viral vector preps with researchers in 45 different countries — and as of 2024, we’ve distributed ...the vectors in Addgene’s catalog are offered in different serotypes. In fact, the 879 viral vectors in our... CRISPR, where we dove fairly deeply into the different types of tools scientists were using/depositing... is less amenable to such analysis, due to its inherent bias: Addgene uses a prioritization process to...service. But why are their request patterns so different? It could be a matter of timing, as jGCaMP7 was...from requestors and depositors, and identifying trends in our own repository data, giving us significant...

...immunotherapy than ever before. Although different types of cancer have different challenges and obstacles to overcome...However, there are many different forms of immunotherapy that work in different ways. For example, a new...learned much about the causes of cancer and the different avenues that can be used to treat it. For those...latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma ...literally thousands of years to Ancient Greece. Current mesothelioma treatment As with many forms of cancer...catch the disease earlier. New techniques are currently being tested in clinical trials to detect mesothelioma...mesothelioma, such as in the case of Paul Zygielbaum. A different approach to immunotherapy is bevacizumab (Avastin...

...in the U.S., whose current salaries are below the new exemption level (the current average postdoc salary...exactly how postdoctoral salaries are changing. Transparent salary information for postdoctoral positions...data) and therefore the numbers of postdocs at different points on this salary range. We have been able...are doing, however, may face greater challenges. Current salary information that we have been able to find... with relative certainty that just over 40% of current postdocs will have their salaries raised to the...already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard group...laws will have on the postdoc population. In the current funding climate combined with the lack of a sustainable...

...more scientists in our leadership, and raising awareness regarding the pitfalls of accepting alternative...outside the scientific community. Vanessa Sung is currently a PhD student studying breast cancer at the Goodman...Over the last few years, there's been a disturbing trend in the number of high profile people denying science...climate change is a hoax. This has culminated in the current President holding and campaigning on many views...Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also studies energy...that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at Vanderbilt University...fingers of skepticism to defend an agenda that halts current data collection, destroys existing data, and guts...

...can't distinguish the different proteins from each other. To distinguish different target proteins in your...target. By using a mix of different antibody-fluorophore combinations for different markers of interest, you...fluorophore. However, these aren't strict rules and in practice the differences in brightness levels are ... fluorophore's brightness refers to the signal strength of the emitted photons detected by the cytometer...a global maximum at around 560 nm, leading to different levels of brightness, depending on the laser light's...floodlight. For fluorophore brightness levels, these differences are much more subtle (think of brightness levels... Even though the two compounds are completely different in nature (eGFP being a protein and FITC an organic...

...numerous variants for the different types of opsins available and their differences and uses have been discussed... the different variants [1, 2, 4]. And, of course, the best way to learn about the many different opsin...particular neurotransmitter release or post-synaptic current. Fortunately, if your lab focuses primarily on ...about this in detail later). But what are the different steps in an optogenetics experiment and what are...Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne... or follow him on Twitter @derekpsimonphd.    References 1. Tye KM, Deisseroth K. Optogenetic investigation... for analyzing the neural circuits of behavior. Trends in cognitive sciences. 2011;15(12):592-600. doi...

... science) they come from many different places supporting many different positions. It would be detrimental...cycle begins again. No part of this process is inherently political so why is there a march in Washington... (scientists who hold a doctorate degree, are currently working full-time, and have received a research... and everyone is welcome. Why? There are many different things scientists and non-scientists alike are...not necessarily marching for every cause. The references provided also only provide a snapshot into the...microbial interactions, and science education.       References March for Science Website Locke, John, and Peter...Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers need...

...  7 ways to strengthen your new relationships Here are some ways to go about strengthening a new relationship...events for one organization or go to the same conferences/meetings over and over to get to know the regulars...often the easiest way to build relationships and strengthen them over time.  Things to avoid when following...starfish shape. You can ask me about the different arms of this starfish sometime, but as of yesterday... was only one other person in my class who had children. When I had my baby, we became closer. She supported...really understood what I was going through. (Parenthetically, when I called the lab to ask my benchmate ...come across your contacts over the years in many different ways. This leads me to my last tip: It’s usually...

...deposits to Addgene.  Insomuch as different journals represent different research areas, target readers,... this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits... would also be great to know how much of that difference in citations is caused by depositing a plasmid...wouldn’t be causal. To control for a number of differences between those papers with Addgene plasmids and..., so our estimates now take into account any differences in citations due to the journal of publication... the same journals! So, is all this remaining difference caused by depositing the related plasmids with...it is still suggestive that we see such a big difference in citations after controlling for journal. And...

...plasmid cloning, and viruses. References Baltrus, David A., and Karen Guillemin. "Multiple phases of ...unique to the lab. Let’s take a look at these different methods of DNA insertion. Transformation Transformation...Various factors promote natural transformation in different bacteria such as growth phase of the cells (Baltrus...plasmid, the genes on the plasmid can give them different phenotypes. For example, this plasmid encodes ...cell. Once inside, phages can follow one of two different life cycles: lytic or lysogenic. Lytic phages ...a common tool used by scientists to introduce different DNA sequences of interest into a bacterial cell...through cell division. You can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use...

... activate different G proteins, work in different neurons and neuroglia, and have different effects on...internalization of two different receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics...Addgene’s chemogenetics guide.     References and resources References Kroeze WK, Sassano MF, Huang X-P,...These receptors can be activated by an array of different ligands, including hormones, neurotransmitters...is estimated that there are approximately 950 different human genes encoding GPCRs (Takeda et al., 2002...

...St Pierre is currently a graduate student at Northeastern School of Nursing and currently works as a registered...low-income communities to educate parents about resources that can help their children thrive academically. Rose...helps you find internships and scholarships in different subject areas!    2. Resume and cover letter...your relationships to the person as well as their current sphere of influence. In other words, the longer...the better, and their leadership status in their current field of expertise augments their authoritative... while you make the ultimate case: why you? 7. Strength in numbers Encourage your close peers to come ...C. St Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program...

...international mentoring conference held at the University of Valencia (Spain). At this conference, mentors and mentees...international mentors. Universities from five different geographical locations in Spain were enrolled:...mentees held scheduled monthly progress video conferences and had real-time correspondence via e-mail throughout...Mentorship Program and the International Mentorship Conference In addition to a pre-program self-assessment ... exchanged impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary...community overseas. Official representatives from different Spanish universities and high level public and...their support and hope for the IMP program. The conference also honored IMP Mentors & Mentees with awards...

...everyone at this specific conference, and I was able to include and exclude different modules for this workshop...something about it. Building a diverse team with different talents and expertise Fortunately, the eLife ambassadors...realized that holding the next one at a scientific conference could potentially enable reproducibility practices...it physically to instruct the workshop at the conference, but because each contributor made the materials...open and modular nature of the workshop content: different parts could be mixed and matched to serve a specific...working with communities, not for them - Another strength of this collaboration is that it is driven by ...the lookout for upcoming workshops at future conferences this year. Workshops are confirmed for the Evolution...

... words, they were leaving these meetings with different interpretations of what we discussed and decided...scientists learn how to communicate their work differently based on their audience. How you talk about your...at a poster is going to vary depending on the conference you’re attending and who approaches you. You ...with professionals from other backgrounds at conferences or networking events. And if you’re a wet lab...fellow team member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell...certainly wasn’t the only problematic word. How many different things do you simply refer to as a “tube”? We... they could in fact be interpreted in several different ways depending on the team. When Tony, our corporate...

...NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three distinct colors...orthologs is key: since each ortholog requires a different PAM sequence, a gRNA designed for one dCas9 should...specific for telomeric sequences and showed that different fluorescently labeled dCas9s are efficiently directed...target sequence. They succeeded in labeling two different pairs of chromosomes using gRNAs specific to sequences...specified by PAM sequence, each color requires a different Cas9 ortholog, and the target sequence must be... green, and red. In contrast, a gRNA with two different hairpins will produce a secondary color cyan, ...sequence (as noted in the table). gRNAs with two different hairpin sequences produce yellow, cyan, or magenta...

...template sequence. 5' and 3' primers will have different leader sequences, but operate on the same principle...

... Evaluating Gene function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing...genes. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to knockdown expression of specific gene...morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids to investigate for ...WISH technique. The expression patterns of 11 different planarian genes (all of which are available through...phenotypes of RNAi knockdown treatment should be apparent after three dsRNA feedings. Some of the phenotypes...and if you make new plasmids for investigating different planarian genes, we encourage you to deposit them...researchers can easily investigate in planarians. References 1. Alice Accorsi, Monique M. Williams, Eric J...

... to run an ELISA in your lab!   References and resources References Boguszewska, K., Szewczuk, M., Urbaniak...Antibodies are used in many different experiments that require scientists to detect proteins in their...enzymatic reaction. Types of ELISA There are four different types of ELISAs, which differ in the way the antigen...antibody and the detection antibody recognize different epitopes on the target protein, so they do not... for which multiple antibodies that recognize different epitopes are available; this type of ELISA is ... are coated with another protein — known as a reference antigen — that can also bind to that antibody....antigen as the other types of ELISAs require two different antibodies. As mentioned above, it can also be...