We narrowed to 601 results for: Ret;
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TypeBlog Post...papers and webpages are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked...Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA...
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Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post..., you’ll first need to learn a little bit of theoretical background on the different parameters that your... tumor sample You made it! So much about the theoretical background. Let’s dive into the praxis of gating... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...researchers realized they needed specific tools to interpret the roles of individual integrins. The Ha lab ... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...This means all newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in...invasive strand is displaced from the template and returned to the processed end of the non-invading strand... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ... -
CRISPR Plasmids - RNA Targeting
TypeCollection...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top Plant ID Plasmid... Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to ...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection... Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant... ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a large selection... -
Serotype Testing AAV
TypeCollection... AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and AAV retrograde....AAV11 37825-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP...AAV11 50465-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde 50465-AAVrg.T 20 µL $ 150 Add to Cart *Each viral... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...good solution. The process of error-prone PCR is pretty much exactly what it sounds like — a PCR, but with...antibodies that target families of cell surface and secreted proteins (glypicans, integrins, and more coming... -
Antibodies 101: Validation
TypeBlog Post...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post... is required in this process because result interpretation might be difficult. The low density of mycoplasmas...by these bacteria can hinder correct result interpretation. Also, the signals from the nuclear area make... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post... try research? Personally, I have always been a pretty curious person. I like to ask questions and think...that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots you don't take” quote... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top Content last reviewed on 01 October 2025... -
mTOR Pathway
TypeCollection...was generated with the help of David Sabatini . Return to top mTOR Pathway - Gene List Click on a name...sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2...complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...entire plate was scanned for V5 tagging using an Operetta imaging system. Dewari et al, eLife 2018;7:e35069...follow when planning epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no.... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post... proper behavioral readout that allows you to interpret the results of your experiment. Like many things...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is... -
Worm Expression Resources
TypeCollection..., or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits are sets...synthetic biology plasmids for use in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans...genome of the genetic model organism C. elegans. Return to top Do you have suggestions for other plasmids... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP AICSDP-57 mEGFP ...SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus...