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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 356 results for: SAM activators

Showing: 281 - 300 of 356 results

  1. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... hybrid vector, as measured by β-galactosidase activity, was 81% of that from a single vector, but significantly...nbt0702-697 Hirsch ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction:...Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized AAV Transductifon Is Mediated...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021). Yu, D...., and deep penetration of red-shifted light. The same NIR FPs and biosensors can be imaged at spatial ...

  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...RNA cleavage, and translational repression or activation: Kellermann, Stefanie J., and Andrea Rentmeister...plasmids used to generate these fusions all use the same restriction enzymes for cloning. Furthermore, some...

  4. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... metastasis as well as teaching and mentoring activities. Follow her on twitter @zcastan or on linkedln... my mentor, since she does research at UK in the same field that I do: prostate cancer. She has guided... Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant expertise and skills...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying antibody... However, the acceptor doesn’t need to go to the same exact location as the donor. As long as some of ...targeted nearby (such as a neighboring house on the same street as the donor), then FRET measurements are...Ebright, 2001). These Cy/NTA conjugates work the same way as NTA-sepharose beads used for protein purification... binding sites are adjacent to each other on the same protein and a negative control experiment conducted...

  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...effects, each group finds very low to no off-target activity at the ten highest-ranking predicted off-target...slightly different methods, they each observed the same positive phenotypes in cardiac and skeletal muscle...

  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...protein level at the timepoint studied Off-target activities of the RNAi Correctly interpreting your data...the abundance of the epitope in the sample, relative to cross-reactive antigens – that is, an antibody shown... recognize different epitopes of the same protein, show the same pattern of labeling, this is supportive...detecting the target-of-interest in your assay and sample-of-interest. The approaches available broadly map...determining the selectivity of antibodies for other sample types and for non-surface (intracellular) antigens...approaches used will be different depending on the sample and target type. To start the validation process...the target at a robust level, similar to that in samples of interest. If you are working with cells that...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were engineered...findings of the 1952 Hershey-Chase experiment. In the same year, Rosalind Franklin and her student Raymond ...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Resources on the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate...investigator can be relatively certain that their samples don’t have off-target mutations if they remain ...

  11. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR...

  12. Chemogenetics Guide

    Type
    Guide
    ...For example, PSAM Q79G,Q139G is activated by PSEM 22S , while PSAM L141F,Y115F is activated by PSEM 89S...of Na + and/or K + ions, and activates neuronal activity. Figure 3: PSAM4-based ligand-gated ion channels...ligands, could be activated by synthetic ligands. Specifically, Receptors Activated Solely by Synthetic...constitutive activity, making them less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer...endogenous ligands, have low constitutive activity, and their activating ligands have few, if any off-target...First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs...off-target activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...

  13. Viral Vector Guides and Plasmids

    Type
    Collection
    ...multi-plasmid system. Many viral plasmids are also self-inactivating, meaning that they can only complete one round...typically do not have the ability to propagate in the same way that wild-type viruses do. For general virus...

  14. Plan Your Experiment

    Type
    Guide
    ...fusion with transcriptional activator (such as dCas9-VP64) Many different activator systems exist, including...including the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes...Knockouts Activation/interference Needs good primer design Western blot Knockouts Activation/interference...the gRNA activity. Be sure to sequence the genomic region you intend to target in your samples to ensure...proteins) Many base editors fused to Cas9 exert their activity within a very narrow window proximal to the PAM...dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently...essential protein domain could abolish protein activity and essentially function as a “knockout,” but ...

  15. AAV Production in HEK293 Cells

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-175 flasks). Cell stacks ... 2 Cellstack 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine...0.08 μg/bp Therefore, for each plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1...overnight at 4 °C if needed. Transfer the entire sample to 3 x 500 mL conical bottles and centrifuge at...round of sonication to avoid overheating of the sample. Mix well between rounds of sonication. Sonicate...

  16. Colony Formation Titering Assay

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G...bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....protocol outlines the seeding of the cells at the same time as the virus-mediated transduction. For some... titer, take the average of multiple dilutions. Sample Data Figure 1: A549 cells were transduced with ...

  17. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. ...complete + 10 µg/mL polybrene. Note, this is just a sample of possible dilutions. You may want to try higher...monoclonal lines from the early polyclonal populations. Sample Data Figure 1: Generation of monoclonal cell lines...

  18. Fluorescence Titering Assay

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10mg/mL), Millipore TR-1003-G ...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C.... titer, take the average of multiple dilutions. Sample Data Figure 1: 293T cells were transduced with ...

  19. General Transfection

    Type
    Protocol
    ...FBS can be purchased already head inactivated or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Low serum medium such as Opti-MEM or Opti-Pro...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....24–48 h before checking for protein expression. Sample Data Legend: Lenti-X 293T cells were transfected...

  20. Lentivirus Production

    Type
    Protocol
    ...FBS can be purchased already heat inactivated or it can be inactivated in the lab by heating to 56°C for...chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip... °C as soon as possible to avoid loss of titer. Sample Data Figure 1: 293T cells were transfected with...
Showing: 281 - 300 of 356 results