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We narrowed to 353 results for: SAM activators

Showing: 281 - 300 of 353 results

  1. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...RNA cleavage, and translational repression or activation: Kellermann, Stefanie J., and Andrea Rentmeister...plasmids used to generate these fusions all use the same restriction enzymes for cloning. Furthermore, some...

  2. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... metastasis as well as teaching and mentoring activities. Follow her on twitter @zcastan or on linkedln... my mentor, since she does research at UK in the same field that I do: prostate cancer. She has guided... Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant expertise and skills...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying antibody... However, the acceptor doesn’t need to go to the same exact location as the donor. As long as some of ...targeted nearby (such as a neighboring house on the same street as the donor), then FRET measurements are...Ebright, 2001). These Cy/NTA conjugates work the same way as NTA-sepharose beads used for protein purification... binding sites are adjacent to each other on the same protein and a negative control experiment conducted...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...effects, each group finds very low to no off-target activity at the ten highest-ranking predicted off-target...slightly different methods, they each observed the same positive phenotypes in cardiac and skeletal muscle...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...protein level at the timepoint studied Off-target activities of the RNAi Correctly interpreting your data...the abundance of the epitope in the sample, relative to cross-reactive antigens – that is, an antibody shown... recognize different epitopes of the same protein, show the same pattern of labeling, this is supportive...detecting the target-of-interest in your assay and sample-of-interest. The approaches available broadly map...determining the selectivity of antibodies for other sample types and for non-surface (intracellular) antigens...approaches used will be different depending on the sample and target type. To start the validation process...the target at a robust level, similar to that in samples of interest. If you are working with cells that...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were engineered...findings of the 1952 Hershey-Chase experiment. In the same year, Rosalind Franklin and her student Raymond ...

  8. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Resources on the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate...investigator can be relatively certain that their samples don’t have off-target mutations if they remain ...

  9. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...

  10. Chemogenetics Guide

    Type
    Guide
    ...For example, PSAM Q79G,Q139G is activated by PSEM 22S , while PSAM L141F,Y115F is activated by PSEM 89S...of Na + and/or K + ions, and activates neuronal activity. Figure 3: PSAM4-based ligand-gated ion channels...ligands, could be activated by synthetic ligands. Specifically, Receptors Activated Solely by Synthetic...constitutive activity, making them less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer...endogenous ligands, have low constitutive activity, and their activating ligands have few, if any off-target...First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs...off-target activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...

  11. Plan Your Experiment

    Type
    Guide
    ...target gene Many different activators exist, including the multi-plasmid SAM system. Select Expression ...application. For example: To activate or repress a target gene using dCas9-activators or dCas9-repressors, gRNAs...effective than dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression of an endogenous...without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting promoter...expression of CRISPR components Short window of CRISPR activity may decrease off-target effects Additional Resources...gRNAs based on predicted on-target and off-target activity A PAM sequence is absolutely necessary for Cas9...high-fidelity Cas enzyme. In addition to off-target activity , it is also important to consider factors that...

  12. Distribution to Industry

    Type
    Collection
    ...partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications PI Don’t See What You...allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer Information...

  13. AAV Production in HEK293 Cells

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-175 flasks). Cell stacks ... 2 Cellstack 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine...0.08 μg/bp Therefore, for each plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1...overnight at 4 °C if needed. Transfer the entire sample to 3 x 500 mL conical bottles and centrifuge at...round of sonication to avoid overheating of the sample. Mix well between rounds of sonication. Sonicate...

  14. Colony Formation Titering Assay

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G...bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....protocol outlines the seeding of the cells at the same time as the virus-mediated transduction. For some... titer, take the average of multiple dilutions. Sample Data Figure 1: A549 cells were transduced with ...

  15. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. ...complete + 10 µg/mL polybrene. Note, this is just a sample of possible dilutions. You may want to try higher...monoclonal lines from the early polyclonal populations. Sample Data Figure 1: Generation of monoclonal cell lines...

  16. Fluorescence Titering Assay

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10mg/mL), Millipore TR-1003-G ...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C.... titer, take the average of multiple dilutions. Sample Data Figure 1: 293T cells were transduced with ...

  17. General Transfection

    Type
    Protocol
    ...FBS can be purchased already head inactivated or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Low serum medium such as Opti-MEM or Opti-Pro...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....24–48 h before checking for protein expression. Sample Data Legend: Lenti-X 293T cells were transfected...

  18. Lentivirus Production

    Type
    Protocol
    ...FBS can be purchased already heat inactivated or it can be inactivated in the lab by heating to 56°C for...chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip... °C as soon as possible to avoid loss of titer. Sample Data Figure 1: 293T cells were transfected with...

  19. CRISPR Guide

    Type
    Guide
    ...helper activators; highest levels of single-gene activation (Figure 9D) In bacteria, activating gene expression...nuclease activity Cas9 enzymes will likely improve as more key residues for specificity and activity are discovered...-NG - NG; increase in vitro activity SpG - NGN; increase nuclease activity SpRY - NRN/NYN, where R = A...select for gRNAs with high on-target activity and low off-target activity. Knockout libraries often target...become a flexible tool to activate, repress, visualize, and isolate genes. Activation or Repression of Target...transcriptional activator (e.g. VP64) or repressor (e.g. KRAB; Figure 9A). Other examples of activation systems...instead of a two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified...

  20. Optogenetics Guide

    Type
    Guide
    ...different colors of light to activate/silence the same neuronal population, or activate different neural populations...respectively. Color of activation light. There are a variety of different activation wavelengths, ranging...including protein activation, membrane localization, and transcriptional activation. In the widely used... transcriptional activation domain and DNA binding domain, allowing light to activate transcription. Phytochromes...Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike ...the closing of the channel 470(activ.), 590(inact.) ReaChR Red-activatable variant of CrChR2 590 Channelrhodopsins...Different activation wavelengths also make it possible to combine multiple opsins in the same experiment...
Showing: 281 - 300 of 353 results