We narrowed to 399 results for: SEF

1. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   ... cholesterol metabolism. The TALEN kit is quite useful in generating the mutant cell lines that one needs...

2. Seven Tips for Using LinkedIn as a Scientist

   Type

   Blog Post

   ... it feels creepy, don’t do it.” It’s just not a useful way to build or use a network. Total strangers ...

3. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   ... replaces a nonbridging phosphate oxygen. Other useful backbone modifications to consider include amide...

4. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   ...reagents can have deeper penetration into a tissue, useful for applications like IHC, or be able to access...

5. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   ...picture problems systematically and logically is as useful for finishing your thesis as it is for helping ...

6. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ... Professor Schuman’s lab showed that FUNCAT’s usefulness can be extended to determine the turnover of ...

7. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   ...used. For example you might find that you can get useful data from your chosen FP for much longer than predicted...

8. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   ...tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy, the...

9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   ...Technique page and the Addgene Blog for a variety of useful resources and protocols! We invite feedback from...

10. Antibodies 101: Fab Fragments

    Type

    Blog Post

    ... Fab portion of the antibody. Fab fragments are useful when you just need an antibody’s antigen binding...

11. To Each HIS Own

    Type

    Blog Post

    ...target for antibody based assays. Antibodies are useful tools for studying proteins and are used for a ...

12. Developing Lab Management Software for Biology

    Type

    Blog Post

    ...just limited to large volume facilities; it can be useful in academic labs where postdocs, students, and ...

13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ... having such fine control of gene expression is useful for many metabolic engineering and synthetic biology...

14. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    ...words and applying annotation can be particularly useful in academic labs when creating shared experimental...

15. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    ...new ways to make this workhorse protein even more useful! From FPs optimized for oxidizing environments ...

16. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    ...minuses, but Golden Gate cloning has been especially useful within both the synthetic biology and genome engineering...

17. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    ...coaches have websites you can review and most create useful online content in the form of blogs or articles...

18. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    ...succeeded at grant writing to see what tips they found useful. Step 4: Read and learn from the reviewers Reviewers...

19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    ... knockout mice. While these techniques are each useful for introducing one modification into the mouse...

20. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    ...resources discussed at the meeting, in addition to useful plasmids and toolkits available at Addgene.  C...