We narrowed to 447 results for: SUP

1. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

   Type

   Blog Post

   ...careers. In that time we may have only 2-3 formal supervisors that will provide guidance and experience. Navigating...

2. Addgene is Expanding Our Viral Vector Service!

   Type

   Blog Post

   ...considerations that may affect expected titers, a customer support specialist will reach out to you during the request...

3. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

   Type

   Blog Post

   ...along with the ability for any user to upload supporting documentation that agrees or disagrees with the...

4. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

   Type

   Blog Post

   ...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...

5. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

   Type

   Blog Post

   ... interests. We do this by promoting citation, supporting sophisticated search, and curating plasmid collections...

6. Mapping the 4D nucleome with CRISPR/Cas9

   Type

   Blog Post

   ...resolution. The combination of this method with superresolution microscopy may improve the resolution, although...

7. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   ...Vagaries of conditional gene targeting. Schmidt-Supprian M, Rajewsky K. Nat Immunol. 2007 Jul;8(7):665-...

8. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

   Type

   Blog Post

   ...on the corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your...

9. An Addgene Summer Internship

   Type

   Blog Post

   ... an environment with limited opportunities and support, puts into perspective just how much one’s environment...

10. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type

    Blog Post

    ...from the bench, look for “technical or product support specialist,” “field application scientist,” or ...

11. Lentivirus Production

    Type

    Protocol

    ...harvesting. Filter supernatant through a 0.45 μm PES filter. The viral supernatant can be stored at 4 ...at 4 °C between harvest. Centrifuge the viral supernatant at 2100 rcf for 5 minutes to pellet any packaging...

12. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    ... imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen also has little sequence...

13. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    ...that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). In the absence of autophagy...

14. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    ... a strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter...

15. Summer SciComm: Science Storytelling

    Type

    Blog Post

    ...ending.    Fig. 1: We all know that the superheroes win in the end. But we’re still invested in seeing...

16. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    ...more! Optogenetics Optogenetics continues to supply neuroscience with ever more efficient tools for...

17. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    ... and close guides for Cas9 and Cpf1. This tool supports over 250 genomes, and they just released a brand...

18. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    ...purified protein involves immobilization on a solid support. This allows washing away of unbound material to...

19. Identifying Sequence Elements with SnapGene's Feature Database

    Type

    Blog Post

    ... are in the pipeline. First, now that SnapGene supports “Collections”, which are shareable databases of...

20. Plasmids 101: TOPO Cloning

    Type

    Blog Post

    ...endonuclease and as a ligase by cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO ...