... antibodies. This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV...anti-AAV antibodies often neutralize the therapeutic efficacy of AAV vectors. How are exosome AAVs produced...interest special contexts, such as the delivery of therapeutic proteins to the brain which requires crossing...

...generally agreed-upon international moratorium on utilizing CRISPR/Cas9 in humans. This topic was to be revisited... unethical experimentation on human subjects, utilizing a scientific technique not proven adequately safe... of the consulting firm Rogue Bioethics. Kelly utilizes her firsthand experience in the software industry...

... to track down that resource. A more long-term solution is the use of unique persistent identifiers (PIDs...CURIEs) combined with well-established prefix resolution services such as Identifiers.org and N2T.net....when citing, you can copy and paste the text attributing the PI who generated the plasmid, followed by...

...researchers are now using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting...suggest it might work well enough to consider as a therapeutic.” There are many fundamental questions about ...Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610...

...between researcher and expert; 4) Credit all contributions.   Our volunteers are extremely diverse....where a researcher makes a small but crucial contribution to someone else’s project. We think that these...right expert and the lack of credit for small contributions. We strive to eliminate these two barriers, ...

... this app is it turns your writing habit into beautiful graphs! I like to look at these graphs when I ... need that extra push to stick with my writing routine. The graph of which days of the week you most often...categorized by the goal of the phrase, such as being cautious, being critical, or defining terms. The phrases...

...systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They...proprietary hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: ...enzymes cannot be used for multiplexing, and the solutions used to provide the chemilumescence may be light-sensitive... the past few years. Pro tip! You only need a resolution of ~150 µm to image a blot. This is because proteins...

...pandemic in which scientists race to find vaccines, therapeutics, and other treatments to stem the spread of ... accelerate research and to help find a faster solution to the COVID-19 pandemic. Though we’ve confirmed...citation to the plasmid page on our website. Contributing your results helps other researchers using these...

...Mutagenesis is a tool that both evolution and molecular biologists use to tinker with DNA. Making changes...help scientists identify and/or facilitate the evolution of new phenotypes, and forward genetics harnesses...rate. For example the Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the...

...cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often a selectable...your construct in target cells transiently or constitutively depending on the virus used. All of these ...libraries makes them the ideal vector for the job. Therapeutic applications Vaccine development Attenuated and...

...computational processing with biological protocols and revolutionary physical approaches. However, with each new ...evaluating antibody staining, or counting cells are now routine, open-source software projects that actively engage...community to regularly replicate designs worldwide. Distributing hardware via globally established makerspace...

...consider the advice below:  1. Eligibility check Scrutinize every opportunity prior to starting an application...program coordinator with your question(s). This precautionary step increases the likelihood of acceptance ...communicates your interest in a specific program, and institution. Consequently it’s best to draft a cover letter...

...sequences. Researchers have also used experimental evolution to develop Cas9s that have a broader PAM specificity... recently, the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9 resulting in a Cas9 with...PMC5951633. Lee, Jungjoon K., et al. "Directed evolution of CRISPR-Cas9 to increase its specificity." Nature...

... plasmids and co-transfected into cells for reconstitution. Image from Levy et al., 2020 with permission...tested the base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse ...Niemann-Pick disease type C, directly demonstrating the therapeutic potential for the split base editors. Find the...

...connectivity patterns of these cells and the functional contribution to a variety of behaviors related to addiction...Image from Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian”) is a postdoc...video-based high-throughput screen for directed evolution of biosensors based on their dynamic properties...

...tet-off turns off constitutively expressed proteins while tet-on turns on constitutively silenced proteins...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting...

...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...The photoactivatable counterpart to this system utilizes a gRNA which is photocaged and unable to engage...initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to control the...

...sources of miscommunication and b) find practical solutions.  My PhD prepared me remarkably well for this...misinterpretation was definitely happening. Our solution for this problem was the creation of an Addgene...sought buy-in from all of Addgene’s teams. This solution can easily be implemented in an academic lab setting...

...participating in science and engineering. “Fix the Institutions” promotes gender equality in careers through... shows that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers... both sexes ensures that the diagnostics and therapeutic interventions you develop represent the whole...

...studies are expected to identify new therapeutic targets/solutions and enable scientists to discover if...sites in T4 lysozyme by deficiency-creating substitutions." Journal of molecular biology 277.2 (1998):...