We narrowed to 564 results for: Vit

...How do I engage my students in real-world lab activities? I’m trying to inspire them and also keep up ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...

...existing components are adapted and combined with creativity and feedback from the community to create state-of-the-art...Madagascar. Platforms that allow for on-going creativity with the construction plans. Appropriate open-source...

...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...the lacZ gene, thus disrupting 𝛽-galactosidase activity. These cells, presumably the cell you want, cannot...

...study the effect of gene knockout in vivo and in vitro, as well as to insert targeted mutations through...gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate deletions ...

... of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing...

...depleting a protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing...performance, great depth of degradation, absolute selectivity, broad utility for degrading targets in different...

...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications in which...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent...

...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all... and taking on independent projects, you will inevitably develop problem solving skills passively. But...

...necessary and sufficient for certain patterning activities of entire tissue types or even organs. In frogs...activation. Upon activation, exhibits stabilized activity for over 30 minutes and can be deactivated using...

...achieve maximal on-target and minimal off-target activities have proven themselves incredibly useful for ...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

...Another option uses optogentics to control neuronal activity, commonly via light-activated ion channels. Similarly...vectors By modulating neuronal gene expression and activity, you can use viral vectors to study all sorts ...

...could in theory promote off-target binding. The activity of SpRY was validated in double stranded cleavage...assessment of the PAM compatibility and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep...

...provides a noninvasive method for manipulating cell activity, especially neuronal firing. What is chemogenetics...Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively...

... scared to engage with them. Comment on their activities and participate in discussions. Get your voice...Twitter to promote your other science communication activities, e.g. tweet it when you publish a new blog post...

...important implications for the fusion protein effectivity), and a fluorescent protein tag for visualization...VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's...

...researchers can explore synaptic integration and connectivity without the constraints of antibody labeling...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

...ABE8s showed a median increase of 1.94x editing activity compared to ABE7.10. Depending on the position...evolution of adenine base editors with increased activity and therapeutic application. Nature Biotechnology...

...polyadenylation generally serves to promote transcript longevity in eukaryotes and promote transcript degradation...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ...

...where each Committee Chair and Vice Chairs were invited to spend a weekend together, funded by supporters...spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee within...