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We narrowed to 466 results for: organization Addgene

Showing: 281 - 300 of 466 results

  1. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...plasmids from this publication at Addgene! Additional Resources on the Addgene Blog Find planarian tools for...without the need to handle a living, replicating organism. Furthermore, cell free systems can be freeze-...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various important considerations...github @tlambert03.   Additional Resources on the Addgene Blog Fluorescence Microscopy Techniques - Which...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find...

  4. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic...

  5. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...multi-color imaging. Find the HA frankenbody tool-kit at Addgene to get started with your experiments! What else... frankenbody can be used for imaging in whole organisms. The researchers used the HA frankenbody to successfully...PMCID: PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover ...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids...

  6. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...All organisms share an innate goal to survive. This past year, scientists hijacked survival tactics of...innovative biological and translative technologies. Addgene has empowered researchers to harness previous experimental...For more information on CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... would like to thank some of the people and organizations who helped us reach this milestone. Viral service... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...

  8. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ... is what got him involved with Addgene - he says he’s “known of Addgene basically since the beginning ...ethic that Addgene has behind it is something that very much resonates with me.” About Addgene’s role in...Doench says, Addgene is “a central resource for what is worth having. The work that [Addgene’s] outreach ...do and to organize it and catalogue it, I mean that’s the critical function really…. Addgene’s role as ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

  9. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding... scientists globally, Addgene has partnered with various community organizations and workforce projects...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...to these researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce...its position in the river back to the user.   At Addgene we're happy to be able to support diverse groups...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... to our viral vector pages on the Addgene website and on the Addgene blog to get some ideas for future...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse... Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...you can apply it in neuroscience. AAV tools from Addgene are already having an impact on the field Not to...horn too much, but many people who came to the Addgene booth (there were over 400 who signed up for our...virus (AAV) and lentivirus are now available from Addgene. While super useful, these vectors require a bit...

  11. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ... on Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox...Many of these promoter systems are available at Addgene! Chemically inducible promoters Chemically regulated... Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter...the promoter, thereby activating transcription. Addgene depositors have developed heat shock-inducible ...allowing reporter gene transcription to take place. Addgene also has popular light-regulated two component ...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...writing of this article. Additional Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids...

  12. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn... the different website navigation paths, the organization and completeness of our information, and the...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...data in particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...effort and cross-team collaboration (thanks to all Addgenies involved). The new homepage and navigational elements...

  13. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages...inflammation for therapeutic benefit ”as stated by the organizers. My overall impression from this conference... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids...

  14. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...Proteins on the other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb...suited than another. Additional resources on the Addgene blog Read more about molecular biology protocols...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

  15. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...about reagents, and why other publishers and organizations have various different versions of checklists...development of Cell Press. Additional resources on the Addgene blog Find more ways to make your research more...

  16. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... reaction is fairly quick, make sure you stay organized and prepare everything you need for the next step...PubMed PMID: 7798275. Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR ...Learn How to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up...

  17. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...PMCID: PMC3433802. Additional Resources on the Addgene Blog Learn About Our Cancer Collection Pages Plasmid...Ghana Modulate Cancer Signalling Resources on Addgene.org Check out Our Cancer Collection Pages Use the...

  18. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...expert to see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the...applications. For more information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR... CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian systems...

  19. The time and cost required to make a plasmid

    Type
    Blog Post
    ...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...where to find them! Keeping good records and an organized lab saves time and money across the board. Consider...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...

  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook...information on Class 2 Cas enzymes. The information is organized in wiki format and includes biochemical properties...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that enzyme... from the article if they're available through Addgene. So whether you're looking for the exact Cas9 plasmid... or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered!     ... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment...
Showing: 281 - 300 of 466 results