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We narrowed to 449 results for: organization Addgene

Showing: 281 - 300 of 449 results

  1. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ...about reagents, and why other publishers and organizations have various different versions of checklists...development of Cell Press. Additional resources on the Addgene blog Find more ways to make your research more...

  2. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post
    ...Proteins on the other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb...suited than another. Additional resources on the Addgene blog Read more about molecular biology protocols...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

  3. Plasmids 101: TOPO Cloning

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    Blog Post
    ... reaction is fairly quick, make sure you stay organized and prepare everything you need for the next step...PubMed PMID: 7798275. Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR ...Learn How to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...PMCID: PMC3433802. Additional Resources on the Addgene Blog Learn About Our Cancer Collection Pages Plasmid...Ghana Modulate Cancer Signalling Resources on Addgene.org Check out Our Cancer Collection Pages Use the...

  5. The time and cost required to make a plasmid

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    Blog Post
    ...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...where to find them! Keeping good records and an organized lab saves time and money across the board. Consider...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook...information on Class 2 Cas enzymes. The information is organized in wiki format and includes biochemical properties...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that enzyme... from the article if they're available through Addgene. So whether you're looking for the exact Cas9 plasmid... or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered!     ... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment...

  7. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral...retroviral plasmids Addgene's collection of AAV plasmids Addgene’s collection of adenovirus plasmids Resources...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...components of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for ...Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids...

  8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...resources on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors...patterning activities of entire tissue types or even organs. In frogs, ectopic expression of ion channels, ...discussed in Mathews and Levin, 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine...

  9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    Blog Post
    ...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and...and thus transformation efficiency. Check out Addgene’s blog to learn about making your own competent ... as well as ready-to-use viral preparations at Addgene. For more information on viral vectors, including...starting any molecular biology experiment check out Addgene’s protocol page, which has both protocols and videos...PubMed PMID: 16357262. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Check... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

  10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    Blog Post
    ...several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-based directed...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including...toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting norovirus will be ...SH3 domain containing plasmids available through Addgene. These plasmids can be used to carry out any biochemical...hundreds of additional plasmids available through Addgene, for study of the of WW, PDZ, and other protein...A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics... Various Addgenies...

  11. Sweating the Small Stuff: Details in the Lab

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    Blog Post
    ...Bench setup Small adjustments in the physical organization of your workspace and your workflow can have...documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide to Getting Started in Undergrad ...

  12. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    ... the effects of the drug on the actual cells or organ system. This allows scientists to get a more definitive...and current Scientist, DNA Sequencing and QC at Addgene.  References and resources References Mosch, ...

  13. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post
    ...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects.     Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

  14. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  15. Viral Vectors 101: Types of viruses

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    Blog Post
    ...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...

  16. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post
    ...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...

  17. Addgene: The First Twenty Years

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    Blog Post
    ... a repository others could request from — Addgene!  Addgene was established as an e-commerce company, ...a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the Society...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, ...young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing service to biology...Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning ...

  18. Plasmid Tools for Microbiome Studies

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    Blog Post
    ...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology blog posts Read about synthetic...Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...

  19. Visualizing Protein Turnover In Situ

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    Blog Post
    ...in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...

  20. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al. “Dynamic...PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling...PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of...PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering...
Showing: 281 - 300 of 449 results