We narrowed to 522 results for: pes;
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TypeBlog Post... Viruses can be produced in many different cell types, spanning both prokaryotic and eukaryotic organisms...viruses can be produced in multiple different cell types, including plants, mammals, and insects. In this...
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Viral Vectors 101: Producing Your rAAV
TypeBlog Post...depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication...collect will depend on the serotype, as some rAAV serotypes are abundantly released into the culture media... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...more suited for prediction of very diverse edit types and cell contexts. Figure 1: High-throughput... diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016/j.cell... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...video and reference on how to design PCR primers. Types of PCR Since the invention of PCR, different PCR... analysis, and RNA detection. A summary of the types of PCR Type of PCR Goal of the PCR method ... -
Plasmids 101: Degron Tags
TypeBlog Post...control methods and compare the commonly available types of degron tags. What is a degron tag and why should...options! Below we will review the 4 most common types of degrons and some of the nuances to each one. ... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...light does not overlap in wavelength. In many microscopes the filters are not narrow enough to distinguish...Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral Applications...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated... -
Lentiviral Vector Uses and Overview
TypeBlog Post...contained a viral envelope to dictate the tropism, or types of cells the vector could infect. Finally, the transfer...envelope have high infectivity in a wide range of cell types, so they’re ideal for work with primary cells or... -
CRISPR Antimicrobials
TypeBlog Post...on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their phages ...Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria. It’s a... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...location, location, location There are many different types of samples at Addgene - DNA sent from depositors...barcodes additionally allow us to store multiple types of tube for each plasmid we have at Addgene. Each... -
Hot Plasmids Spring 2024
TypeBlog Post...enhanced editing efficiencies in a number of cell types at different genomic loci — in some cases, over ... Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid protein, one of... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...regions. They also don’t give any clues about the types of neurons involved or whether their involvement..., et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...surprising because in vivo transduction of AAV serotypes generally tracks between strains of mice. This...between these two mice might drive their divergent phenotypes. Two labs, the Deverman lab at the Broad Institute... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...mesoderm is what gives rise to different tissue types in the developing embryo (Azplazu and Frasch, 1993...mutants vaguely resemble a bagpipe and historically, bagpipes were made using the stomachs of different animals... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...immunotherapy than ever before. Although different types of cancer have different challenges and obstacles... mesothelioma make it different from many other types of cancer. Mesothelioma is considered an orphan ... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...collaborate whenever it faces an important challenge. The types of projects mentioned above are just examples, and...leverage the preferences of different personality types, and to devise strategies to be prepared for addressing... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...variety of photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...supports a variety of processes in different cell types. One great way to do this is to use CRY2-CIB1 clustering... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... career scientists aren’t trained to have these types of negotiations. They usually turn into “win-lose...sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all ... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog Post...unique surface epitopes for many proteins, but some proteins do not have such epitopes. These classical... -
Plasmids 101: Repressible Promoters
TypeBlog Post...transcriptional activation domain VP16 taken from the herpes simplex virus. tTA binds to promoters containing...Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. ...