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We narrowed to 535 results for: val

Showing: 281 - 300 of 535 results

  2. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... of the journal club?” You also likely want to evaluate the “success” of the journal club. This can be...journal club’s usefulness should be continually evaluated and assessed for relevance.  Concluding thoughts...

  3. An Introduction to Adenovirus

    Type
    Blog Post
    ...likely for adenoviral vectors – namely due to the removal of certain adenoviral genes that apparently contribute...36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy, K. C., Hensley, S. E., Zhou...

  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...whether you are planning your own ExM experiment or evaluating ExM images in a new publication.         Why .... 2015).  Despite these limitations, ExM is a valuable tool, especially when paired with complementary...

  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...Einstein might have put it best when he stated, “the value of an education . . . is not the learning of many...rejected, etc.), while reminding participants of the value of this approach and encouraging them to engage ...

  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ... to identify knock-in clones by reading up on validating genome edits to find screening strategies for... Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out our CRISPR...

  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...While this methodology has proven to be incredibly valuable to scientists, there are some potential complications... Addgene.org Browse all CRISPR plasmids Find validated gRNAs Check out our CRISPR guide page ...

  8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide...and manipulating proteins in live cells through covalent labeling." Nature chemical biology 11.12 (2015...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...replicates and controls (see Tips below), and by validating leads with other methods. More common are false...your binding. And most importantly: Independently validate identified binding partners with other techniques...

  10. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...simple and efficient way to overexpress a gene and evaluate its function. In nature Agrobacterium contains...PMID: 19183892. De Ruijter, N. C. A., et al. "Evaluation and comparison of the GUS, LUC and GFP reporter...

  11. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...two direct repeats which can be used for marker removal so that the marker can be reused to introduce multiple...recognized and cleaved by RNaseIII resulting in cell survival. Plasmid addiction systems can be great additions...

  13. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... been full of bad news and dreadful prognoses. Survival rates for mesothelioma are creeping upwards, and...greater impact on the life expectancy and overall survival of mesothelioma patients. However, no matter how...

  14. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http://wesharescience.com...audiences that may otherwise never learn from the valuable research we are doing. Browse the site, learn ...

  15. Bioinformatics at Addgene

    Type
    Blog Post
    ...output to link the processes correctly. After this validation check, we repeated our strategy and copied a ...the Addgene blog:  The Power Behind NGS Plasmid Validation: SeqwellHow to Keep a Lab Notebook for Bioinformatics...

  16. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...accessible. The yields from magnetic beads are equivalent, if not slightly higher, than those of agarose...References and resources References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal...

  17. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of the reasons ...https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector...

  18. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...from Addgene!   Figure 1: Experimental validation for Anti-V5 [IPI-SV5-Pk1] in a western blot assay...efficiency of this dual-plasmid approach makes it a valuable resource for advancing our understanding of its...

  20. Hot Plasmids - November 2023

    Type
    Blog Post
    ...click chemistry reactions instead of biotin — a valuable development in and of itself. After adding one... reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D Atlas.  ...
Showing: 281 - 300 of 535 results