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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 580 results for: vit;

Showing: 281 - 300 of 580 results

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated by glutamate...they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation...

  7. How to Lead a Great Meeting

    Type
    Blog Post
    ...impacted by a project. Instead, invite the experts on the topic at hand. Invite one “ambassador” from each ...

  8. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... including their potential toxicity and cross-reactivity with other pathways. Since these effectors are...chromosomally. Leakiness was also an issue; some promoter activity persisted in the inactive state. The dynamic range...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ... instead of having all the pages in a Curricula Vitae to showcase your publications, academic performances...perhaps you were involved in some extracurricular activities – did you volunteer to do science outreach and...

  11. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ... lower background levels and improved signal sensitivity. Another advantage: luciferase reporters aren...Not required Signal strength Lower Higher Sensitivity Higher Lower Background Lower Higher Photobleaching...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    .... Envelope gene (may be pseudotyped to alter infectivity): The use of the VSV-G envelope provides the ...tropism envelope protein; Pseudotyped to alter infectivity. Packaging Gag Precursor structural protein...

  13. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...existing components are adapted and combined with creativity and feedback from the community to create state-of-the-art...Madagascar. Platforms that allow for on-going creativity with the construction plans. Appropriate open-source...

  14. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...How do I engage my students in real-world lab activities? I’m trying to inspire them and also keep up ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...

  15. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous publications...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...showing that Cpf1 can cleave an array of 4 crRNAs in vitro and when expressed in 293 cells. This allows for...effective method with multiple applications (in vitro, in vivo, transfection, viral transduction) for ...

  17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...the lacZ gene, thus disrupting 𝛽-galactosidase activity. These cells, presumably the cell you want, cannot...

  18. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient...

  19. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses researchers...mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes et al., 2011). The various ...

  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...study the effect of gene knockout in vivo and in vitro, as well as to insert targeted mutations through...gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate deletions ...
Showing: 281 - 300 of 580 results