We narrowed to 653 results for: EPO
-
TypeBlog Post...the source or were deposited when the now-published article was a preprint. Depositing plasmids that were... great way to do that. We are happy to receive deposits of plasmids that have been described in a preprint... authors can include the Addgene ID for their deposited plasmids in the final journal publication or an...
-
Plasmid Tools for Microbiome Studies
TypeBlog Post...microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these ...is a ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering... reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition....org Check out our microbiology resource page Deposit microbiology plasmids with Addgene Find synthetic... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...getting support from depositors. We regularly hear how much they see the value in depositing and they are increasingly... own labs get excited about making their first deposit to Addgene. We are also lauded by another set...large community of scientists who support us by depositing, requesting and spreading the word. Our blog... -
Hot Plasmids - October 2020
TypeBlog Post...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. Here's what you'll find in...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...scientist. New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...your own Fragmid component (which you can also deposit with Addgene!) We’re excited to help the Doench... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...experiments in planarians with their recent plasmid deposit. These plasmids enable two main types of experiments...expression of specific gene expression. The 49 deposited plasmids enable students to study gene expression... genes (all of which are available through the deposit) are described in the Sánchez lab publication [...different planarian genes, we encourage you to deposit them back with Addgene and expand the number of... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids...future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic ... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements... -
Hot Plasmids February 2024
TypeBlog Post... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Here's ...PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...Arokiaraj (Gradinaru lab) Another useful viral tool deposited by the Gradinaru lab, AAV1-X1, was developed by... -
Hot Plasmids: Winter 2025
TypeBlog Post... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Here's what...2024 under a CC-BY 4.0 license. The team deposited an array of constructs for tagging the kinesins...Hao and Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt. ...