We narrowed to 641 results for: ato

...specific genes. Many years ago, people in the laboratory became frustrated with using glass cloning rings...sterile truncated pipet tips. Indeed, an experienced operator can pick 48 clones from a 10 cm dish in 15- 20...YouTube video. Before returning the plate to the incubator, additional growth medium is added to about halfway...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties...Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical School in...

...targeted tdTomato and pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus...ready-to-use viral preps with more on the way. S5E2 regulatory element containing vectors developed in the Jordane...Flp-dependent DREADDs viral vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available...

...is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like an engineer ...the toolkit. We have included a wider range of terminators than are commonly used as we have tested them...system. The paper also gives quantitative data for regulatory elements, which has not been available for plants...easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have...start. They might want to use our promoters and terminators, but then they aren’t sure how to convert their...

...in a year-long biochemistry laboratory : Part II – research-based laboratory – a semester-long research...confidence in their research capabilities and laboratory and critical thinking skills (Knutson et al.,...improvisation and the latter making him a more effective motivator (“be like Tony Robbins!” he encourages faculty...research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification and characterization...

...probably never happen. Yet with transcription activator-like (TAL) effectors, scientists in several biological...

...upregulate an alternative pathway or induce other compensatory mechanisms. How can protein function be dissected...protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from Scot Wolfe's lab. .... These include Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014...basic parts (promoters, UTRs, coding sequences, terminators, etc) into individual Level 0 plasmids, or choose...unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, up to six Level 1 modules can be similarly...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...of the gene of interest, C-terminal tag, plant terminator, and plant resistance cassette) in pUC19 based...

... are successful base editors in rice, tomato, wheat, and potato. Target-AID and rAPOBEC based CBEs are... HDR methods for rice crop improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor,...Zenpei, et al. "Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion."...The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018). Additional resources on the Addgene blog...

...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...Einstein College of Medicine in the Department of Anatomy and Structural Biology. His interests include the...Medicine. Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent...

...blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen." Scientific...production of a downstream peptide, the transcription terminators in this series are just the tool you need. The...this portion of the BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP ...characterized in Cambray et al., 2013. These terminators can be used to precisely tune your polycistronic...Kit for some insights on its potential from its creators. We hope you’ll find these kits useful in your...

...list from your current P.I., lab manager, or administrator containing all of the materials that you’ve ...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...