We narrowed to 1,015 results for: LEA

...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...older than those available from biorepositories leading to genotypic and phenotypic artefacts. The life...commercial repositories and the same cell line from a colleague?”. The main difference is the QC and validation...usually the case if you obtain a cell line from a colleague. Time and money are wasted, papers are retracted...

...remains unclear whether the FP misfolding impacts fusion protein function and this could lead to under...localize [4]. Most investigators are surprised to learn that FPs are often poorly suited for cellular compartments...glycosylation, disulfide bond formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible... dark fluorescent proteins in your experiments, please consider the following: How can one ensure that...

...bit of a steep learning curve and many opportunities for mistakes. The easiest way to learn optogenetics... is an approximation of reward or the sense of pleasure that the animal perceives through taking a drug...University.  His interests include the role of learning and memory in opioid addiction and science communication...Kheirbek, Mazen A., et al. "Differential control of learning and anxiety along the dorsoventral axis of the...

...Bench series was born from the shift to remote learning due to COVID-19. We had many protocols for plasmid...details about personal protective equipment to learning about how to use a tissue culture hood and other...other lab equipment, we’re here for you as you learn the ropes! All material can be found on our Protocols...Protocols page and YouTube page.  Happy learning, and welcome to the lab bench, new scientists!   ...

...investigating HIV integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous variants...However, IDLV genomes are transcriptionally active, leading to transient expression of gene products (Wu et... insertional mutagenesis. This integration could lead to an undesirable interruption of a critical gene... off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822...PMID: 18688286 PubMed Central PMCID: PMC2496964 Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H....

...warm meal on a winter day, you should be happy to learn that many DNA-based reagents are named for soup ... and non toxic to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative, but...regions in the 5’ UTR of mRNA that bind metabolites, leading to conformational changes that control the expression...the Corn-DFHO complex. For dessert (last but not least), the only fruit-named aptamer, Mango, binds to ...PMC4143336. Additional resources on the Addgene blog Learn more about aptamer fluorophores Investigate other...

...the uses of biotinylation in molecular biology! Learn about in vivo biotinylation of bacterial fusion ...biotin-avidin complexes bound to your protein of interest leading to amplification of the reporter signal and easier...microenvironments. There are also reversible and cleavable biotinylation reagents to help with the specific...vivo biotinylation of bacterial fusion proteins Learn how biotin is used in Split-BioID: An improved method...method for studying protein-protein interactions Learn how biotin can be used with CRISPR Additional resources...

...which is then cleaved by RISC and degraded. In the miRNA pathway, RISC doesn’t always cleave the mRNA — ...hairpin shRNA structures that are processed by a ribonuclease called Dicer. In a great simplification of the...protein is what binds the small RNA and either cleaves the target mRNA or recruits other silencing proteins...no tissue specificity, so in some cases they can lead to toxicity and off target effects. More recently...

...expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation Improves plasmid...cell mcrA, mcrBC Inactivation of pathway that cleaves methylated cytosine DNA Allows for uptake of foreign... mrr, Δ(mcrC-mrr) Inactivation of pathway that cleaves methylated adenine or cytosine DNA Allows for uptake...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...

...blog posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about... serotype, injection site, and incubation time. Learn more about the Data Hub in this blog post. Alongside...attending SfN2019, stop by booth #2163 to say hi and learn more about Addgene’s viral service. Additional ...

... the Addgene Blog Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris...needs. For more context, exposition, and detail, please refer to the paper. Why might one be interested...uploaded to Addgene. We also recommend inserting self-cleaving hammerhead ribozymes directly upstream of the ... transcribed gene and its 5’ UTR. Various self-cleaving hammerhead ribozyme parts are described in Nielsen...

... I had found my dream job, because one thing I learned in grad school was that scientists have amazing...at their lab benches. It’s these exchanges with leading scientists (and our growing collection of 25,000...sciences research for the past decade. We’ve also learned along the way that a connected and collaborative...scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and get practical tips...

...Health and Allied Sciences in Ghana. Read on to learn more about doctors Bengyella and Duedu and how they...tumors is “a major public health problem, and a leading cause of hospitalization for gynaecologic disorders...Program Additional Resources on the Addgene Blog Learn about Plasmid Awards to Researchers Working on Rare... in China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International...

...alcohol-based disinfectants. A 10% bleach solution should be used to clean AAVs from workspaces. Speed...Manitoba due to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are...vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune response. Any investigator who ...antisense oligonucleotides (ASOs), zinc finger nucleases, and many others (12). The most attractive potential...

...LTRs) has been removed, Rev (which facilitates nuclear export) is expressed from a separate plasmid, and...mutation or recombination with human retroviruses could lead to RCL. Regarding transduction efficacy, no differences...Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant and...retrovirus packaging cell lines to avoid recombination leading to helper virus production." Molecular and Cellular...5 Tips for Trouble Shooting Viral Transductions Learn Why You Might Want to Use AAV in Your Research Lentiviral...

...even to not bind to their target molecule at all, leading to inconclusive results, erroneous conclusions,... that your antibody has been validated using at least one of these strategies. Choosing which strategy...) the protein of interest in your sample, should lead to reduced or eliminated antibody signal. If you...translation start sites or alternative splicing that could lead to some form of the target protein to be present... Check out our Selecting an Antibody blog post! Learn more about Antibodies and their many applications...

...countries. We, like everyone around the world, are learning to navigate the changes brought about by the pandemic...opportunities. I have spent the last 9 months quietly learning about Addgene’s past and present, but the real...successes to reflect upon, a wealth of history to learn from, and a compelling future to plan in the changing...people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger scientific...

... change? Addgene has you covered. Today we’re releasing 11 bold science-themed backgrounds! Show off your...out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other techniques using FPs! ...Addgene are shipped as bacterial stabs? You can learn about what format your material will come as (and...molecular biology tools in your research or not! And learn more about the variety of careers available to scientists...

...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...with the plasmid post transfection. The 2A "self cleaving" peptide causes the ribosome to translate Cas9...gRNA that will target SpCas9 to the approrpriate cleavage site). Finally, in addition to the 2A peptide ...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some...

...Resources at Harvard Medical School, Joanna was my colleague. She mentioned Addgene was looking for someone...jewelry making, reading, sailing, and gardening. Learn more about Julie Cicalese  Ms. Cicalese served as... for Harvard Medical School where she brought leadership teams together to advance the mission of the ...England. Additional resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene"...

... guide Learn about different careers in science communication Resources on Addgene.org Learn how Addgene...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com.... Sadangi is an eLife ambassador, SfN community leader, and an early research career panelist at BioTechniques...

...applications was still very new. Now, Konermann and her colleagues including Zhang and Mark Brigham have taken the...influences in our environments. We are able to react and learn. All of this requires genes to be regulated dynamically...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...processes and disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience...

... well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability...Who even regulates them? You may be surprised to learn that it hasn’t even been 50 years since the creation...fluidic machines handle biological samples and are cleaned between each use. You wouldn’t throw away your ...series!   Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe...

...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights...section to make this post even more useful to your colleagues. Technique: Probe Phage Genomes for Host Binding...(check out our interview with Michael Koeris to learn how his company uses phage to test for Listeria ...posts in chronological order here: Four Things I Learned at the 10 Years of iPSCs Symposium Tips for Writing...

... passion, and patience are required to build and lead a productive and successful research lab. Once goals...champagne, that's a given.” Furthermore, anyone leaving the lab receives send-off via a gift, card, and... the afternoon during the child-rearing years, learning “lots of interesting things from children, their...their classmates, and parents”. He also cooks and cleans with his family, “sometimes listening to audiobooks...

...functionality can be found in the following video: Please remember that the accuracy and reliability of our... plasmid is missing from our collections pages, please shoot us an e-mail at [email protected]. We’d love...visibility in a few other ways. Every quarter we release a Newsletter where we spotlight some of the plasmids... in the repository. And last, but certainly not least, we often feature new plasmid technologies on our...

... also have the option to “Leave us a message.” When you click on the "Leave us a message" button shown...lab’s materials and requesting them from your colleagues, to intellectual property rights and handling...the Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources...

...insights discoverable. Watch this quick video to learn how ReFigure works and continue reading for more... and again as they don’t save the insights they glean from a reading session. To remember what happened...reach the ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering...video to share your science at We Share Science Learn more about Data Sharing Resources at Addgene.org...

... you want. But how do you know if a masters will lead to a career you will like? Here’s two things to ...setting up an informational interview with them to learn how their master's degree prepared them for their... help with manuscript and grant preparation, and lead your own research project.  3. Do you prefer being...eBook.   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now...

... year. The society facilitates opportunities to learn about Synthetic Biology and exciting new technologies...network of fellow scientists that you can grow with, learn from, and mentor while pursuing your research interests... which would benefit from some Addgene support? Please get in touch, as we aim to support a couple of ...Networking Get Career Advice from Addgene Bloggers Learn How to Get Involved in a Profession Organization...