...Addgene’s compensation decisions. It was a great time for me to join the committee because I had recently...community? Access to plasmids which gives people more time to focus on their research.  How will your prior...that I’m not working so that I’m available to spend time caring for my mother. For fun, I enjoy pilates, ...

...meetings, and finding the best way to organize your time. At a conference, you could be honing your science...can't wait for opportunities to fall in your lap. Sometimes, in order to do the thing you love, you have to...  Break the process down into steps and set aside time during the week to tackle each one.  Get the toughest...

...you need the right experimental materials. Who has time for paperwork? Here’s why a Material Transfer Agreement.... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!...Keeping: Addgene will let you know exactly how many times your materials have been requested and by whom. ...

...technology with the Golden Gate method. For the first time, it was possible to quickly and efficiently assemble... in subsequent rounds, dramatically reducing the time required to generate multi-construct transgenic ... half a year,” in comparison to more tedious and time-consuming traditional cloning methods, Lohmann said...

...are involved. They may feel they are wasting their time. The manager may appear to be too busy to be receptive...team: Solicit feedback regularly and often A one-time event doesn't work to get people sharing.   Frequently... for feedback and input on multiple topics every time you meet.  Seek feedback in multiple formats      ...

...knockout mice, which is time-consuming, as it only changes one gene at a time. CRISPR-based systems enable...functions in different cell types, at different times, and in combination with different genes. Having...

...academic inertia, it’s time to develop systems that give trainees a plan, both for their time in the lab and ...students and post-docs alike are wondering where the time has gone. Are we any closer to graduating, publishing...

...just the “training” portion of our careers. In that time we may have only 2-3 formal supervisors that will...think you can finish that book in 4 weeks!)” “Sometimes a mentor is a sounding board--a good mentor asks... risks A great Mentor is often one that devotes time and energy to thinking about how to be a great Mentor...

...out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence.... Addgene's "How to cite this plasmid" section Anytime you use a plasmid from the repository, we request... to repeat the modification. This will save both time and money. A quick note -  While it is tempting ...

...accelerate your research. We know you don’t have time to be waiting on hold, let alone waiting multiple...support team available whenever you need them, but sometimes that’s not enough. With your busy schedule, you...features that allow users to help themselves, saving time and effort for everyone involved. We’re adding self...

...on molecular features found in nature, with the ultimate goal to improve the diagnosis of disease, and ...purchase and use the SpyLigase system for the first time? Howarth: The Proceedings of the National Academy...17] should cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with Addgene...

...fresh insights and broaden your understanding, ultimately enhancing your project's potential. Widening ...mindful meditation, taking field trips, and spending time outdoors in nature or visiting museums can be excellent...ideas. It's beneficial to carry a notebook at all times (or use the notes app on your phone) to capture ...

...methods missing something? Here we will review some time-tested gene identification systems that may have...expression levels (high/low) and patterns (tissue/timing) can be monitored under various conditions without...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...

...a modification of interest. While the authors optimized the workflow for use in a fetal-lung derived organoid...are costly, non quantitative or require heavy optimization. Claudia Vickers’s lab has designed novel SL... genes with 10 gRNAs per gene. The guides are optimized to maximize on-target cleavage and minimize off-target...

...conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

...so that you can spend less time researching the basics and spend more time developing cleverly designed... Gateway, Gibson, etc). The cloning method is ultimately chosen based on the plasmid you want to clone...

...helped grad students who were teaching for the first time. I walk[ed them] through their lecture, giving them...interests. “So for me, I researched G-proteins at the time. This program takes what you know about it [your... I was. It was really luck of right place, right time, and having a good interview experience.” Although...

...more likely) impossible to find; therefore all the time spent collecting it will have been worthless. But...ASM CEO Stefano Bertuzzi welcoming us. Then it was time to work! Set up similarly to the online class, we...technique, then type up the materials and methods. Over time that portion of the paper will be generated. After...

...Check out Joanne's Reddit AMA  Few of us spend much time thinking about conserving resources in the labs....wasteful packaging.  Turns out, one could make a lifetime supply for about $10 US dollars worth of glass... our protocol for making competent cells to save time and money. My other stingy science project started...

...whether an advisor will be a good mentor: Spend time with the potential advisor and don’t be shy about...lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is a warning...if the PI does not welcome you to visit and spend time with everyone. Additionally, if you are looking...

...antibodies that have almost certainly already been optimized for your application. Depending on the tag, directly...of epitope tags exist – all with individual optimizations and specific monoclonal antibodies. Below we... acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s...

...undergraduate degree (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience...findings of brain science, my curiosity did not ultimately translate to a desire to do the research itself...percolate within a new intellectual environment. Over time, this interest came to incorporate other facets ...

...communication research.   This summer, we’re making time to talk about my favorite thing - science communication...knowledge than average and are more likely to spend time engaging with science communication efforts.  Scientific...about science communication itself! See you next time!  Additional resources on the Addgene blog 10 ...

...something Stegmeier has been interested in for a long time. “At an early age, I was fascinated by the impact...therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass... walls we’ve built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe...

...Science Magazine. Although we only walk one path at a time, the variety of paths in life is mind-boggling. ...aspects of bioscience into context, while at the same time working through the details of experimental design...discoveries into an entertaining podcast? Look at our multimedia producer and online editor. And, the copyeditors...

...this; however, you should reduce your incubation time on ice to 5 minutes (incubating the full 30 minutes...group! You may want to include extra extension time after the last cycle of PCR to make sure that the... is not recommended that you exceed the 5 minute time limit (lower transformation efficiencies have been...

...launched in the early spring of 2015 and since that time have received about 3 project proposals per month...making connections and finding mentors -- well, it's time you call upon these people for guidance.  Before... evolve and respond to feedback throughout its lifetime.  In this way, you will always improve and give...

...University of Cambridge and knew it was time to leave. I really enjoyed my time in the lab, my colleagues and what...choices were mostly by chance. Luckily, around the time I decided to look for work outside of academia, ...

... job. EAs shouldn't be viewed as an unproductive time spent away from the bench, but as an opportunity... conferences and seminars, as this is a valuable time to network Attend informational interviews to ...required qualifications and skill sets Taking the time to identify and research 2 – 3 potential professions...

...easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation ... K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering...cerevisiae and S. pombe - aren’t going anywhere anytime soon, and for good reason! However, there are other...