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...Excitation (dotted) and emission (solid) spectra for various teal, green, yellow, orange, and red fluorophores...Figure 4: Calcium titration curves for OCaMP and various red and yellow GECIs measured with soluble protein...

... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ...supply of transgenic reporter lines that supply a variety of GFP expression patterns, thus allowing you to...

...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

...plasmids can direct the production of virus with various tropisms.  Packaging using helper-free packaging... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...

...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples... research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene...

...exhibition area, I got a sampling of the broad variety of tools in neuroscience trade. There were, of ...easily I could jump in and ask questions about the various posters I visited. If you’re interested in neuroscience...

...classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current ...100 teachers each year, with workshops held at various locations around the U.S. (and Mexico in 2018)....

...University of North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the... 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services. There ...

... available.  We’ve also had some requests for variations on the plasmids we’ve developed. We’re working...be doing something similar. There may be other variants of this that are better for some experiments than...

...the degree to which genes are turned on and off varies depending on which guide RNA we use. What we have...straightforward to apply the new library for use in various cell types and in live mice. Therapeutic uses in...

...is going to vary widely. The way you communicate your science at a poster is going to vary depending on...

...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

...reactions that work in just about any situation (Devaraj & Finn, 2021). In one of the most common Click ...clicking. References and Resources References Devaraj, N. K., & Finn, M. G. (2021). Introduction: Click...

...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

...interest. Thanks to the tremendous efforts made by various research teams to build and constantly improve ...integrated, we look forward to the realization of a variety of perturbations and applications in functional...

... either 3-base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see... to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly, which used only ...

... deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to ...

... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

...process and includes Picogreen quantification of various intermediate steps, a sizing electrophoretic gel... the pool but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA...

... these modules (linker length can and should be varied as it can have important implications for the fusion... Various Addgenies...

...were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells grown in high concentrations... Various Addgenies...

...CRISPR activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes...factors to increase gene expression. CRISPRa methods vary in their transcriptional activators: some methods...

...for identifying, categorizing, and resolving a variety of QC issues. This process ensures that any potential...making the same decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited...

...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...

... Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...–10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1–10) Improved split fluorescent proteins...