We narrowed to 731 results for: Sis;

...validate your new construct by Sanger sequence analysis. But for this research, it was likely that the...

...data in a manner that demands high accuracy and consistency?  If the answer to any of these questions is...

...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...

...involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu group at the University ...

...reviews on blog posts are done on a case-by-case basis, as many of our blog contributors primarily write...

...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...

...current and future features being built for the analysis software program, and meet with the automation...

...transcription factor. It also gives cells antibiotic resistance to neomycin so that you can select the population...

... Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships...

...plant synthetic biology In addition to public resistance, an overall limited understanding of plant biology...

...company won’t go anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and...

...programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet together...

...CRISPaint Gene Tagging Kit SpyTag/SpyCatcher CRISPR-assisted insertion tagging Kit; integration of large DNA...

...the level of gene expression. For example, the synthesis of deoxychromoviridans requires three genes whose...

...protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological...

...secondary antibody. Chicken IgY This RANbody reporter consists of two chicken IgY domains. Chicken IgY domains...

...Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ...

...salary information as of December 1st. Salary inconsistencies across geographical regions When we originally...

...phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light (650 nm...

...removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible degron (AID)...

...papers you could get your hands on, formulated a hypothesis, and planned out your key experiments. Unsurprisingly...

... assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification ...

...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...

...you can often remove the incompatible agents by dialysis or gel filtration.    There is, of course, a lot...

..., nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are small and...

...foundation for postdoc-related data collection and analysis. He is also a strong supporter of science Master...

...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

...a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ...

...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...

...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ...

... codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes...

...contribute to our understanding of diseases and even assist in drug discovery. References 1. Baird, Geoffrey...

...Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein...

...: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ...

...integration within the target cells, ensuring more consistent and reproducible results in calcium assays. There...

...ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic...

...backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck and happy...

...goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To...