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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 731 results for: Sis;

Showing: 601 - 640 of 731 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...present, YFI is inactive, preventing repressor cI synthesis and allowing reporter gene transcription to take...

  6. Hot Plasmids - May 2022

    Type
    Blog Post
    ... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific...

  13. CRISPR Library Amplification

    Type
    Protocol
    ... on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not suspended...sufficient DNA for use, pool samples, and continue with analysis. “Sufficient” DNA will vary upon intended use....

  14. Antibodies 101: Conjugation

    Type
    Blog Post
    ...you can often remove the incompatible agents by dialysis or gel filtration.    There is, of course, a lot...

  24. dTAG - You're it!

    Type
    Blog Post
    ...protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological...

  30. Design Tips for Prime Editing

    Type
    Blog Post
    ...a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ...

  31. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...plant biology to achieve traits such as disease resistance. MoClo is a great tool to deliver many combinations...

  34. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic...

  35. 6 Tips for Grant Writing

    Type
    Blog Post
    ...proof-of-principal study were very helpful in assisting me with applications for much larger fellowships...
Showing: 601 - 640 of 731 results