We narrowed to 933 results for: KIN

...high fidelity during action potentials of fast-spiking neurons. The same A-to-D mutation in the related...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...

...tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations...

...lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...overly confluent or too old. Ensuring that you are working with healthy, happy cells is one of the easiest...

...positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...of Replication The Promoter Region – Let's Go! Looking for More Plasmids 101 Articles?  Find them all ...

...biologist you would say. The student was of course talking about the now well-known FRET, aka Fluorescence...and the extinction coefficient of the acceptor (linking the quantity of absorbed light, at a given wavelength...

...thoughts on research statements. Snapp, who is always looking for ways to improve his eBook, adds: "If you have... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...

... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

... are equal. Created with BioRender.com. This kind of normalization is a nearly ubiquitous tool, but...controls So how can you address these errors without making incorrect assumptions? The answer depends on the...

...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...

..., termed BASU. BASU exhibits 1,000 fold faster kinetics and increases the signal to noise ratio 30-fold...more recently cell-cell interactions. If you’re looking to identify interactions with your favorite protein...

... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune, and/or inflammatory...

...optimal promoter for your gene of interest, painstakingly cloned into the perfect empty backbone, made...Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles?  Find them all ...

...cell types and are useful if you're going to be working in several different systems or want to transduce...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

... MQ1.) The Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24...Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation...

...riboregulation could be a promising strategy for linking RSM inputs to endogenous gene expression (2). ...terminators, promoters, and genes) were built with flanking primer binding sites (designed to have 20-30 nt...

...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii...

...gRNAs to choose from. With so many possibilities, picking a gRNA with an optimized sequence is of primary...detectable mutations (Veres et al., 2014). When working with single-cell clones, the authors note that ...

...mutations are essential data points for investigators looking to definitively link genotype and phenotype. It... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

...with DIO/FLEx vectors and off-target expression making it easier to determine the spread pattern around...false negative or conclude the viral vector is not working.  When using a “cell-type specific” promoter, such...

...photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the smallest structures...microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic or static...

...cytometry particularly well to complex experiments looking at a variety of different targets. If you are planning...43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current...

...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ... 98-99% target modification in primary T cells, making them a promising tool for cell therapy applications...

...efficiency is so high that some applications may permit working with edited “pools” of cells, eliminating the time-consuming...telomere length. Telomere trimming can be assayed by looking for the production of ECT-DNA (extra-chromosomal...

...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...

...for Protein Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI...

...If you’re looking for the best way to improve your lab techniques — but perhaps are a little limited ...

...friend.  During the month of January, we’ll be taking name suggestions using this form or the hashtag...

...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...

...recently chosen four names as finalists. Now, we’re asking you to help us make the decision between some meaningful...

...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...

... has a large international audience, including working with distributors across the world to help increase...

...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...

...dressed as Santa, delivering gifts to the Mushroom Kingdom. Kravitz Lab 2019 Holiday Door is up and running...

...keep track of reagents in your lab. With barcode tracking, you will always be able to locate materials quickly...

... than commercial ladders. Are you interested in making your own DNA ladder? You can find the Tan Lab’s...

...processes.  Our most recent protocol video detailing Streaking Plates for Single Bacterial Colonies can be found...

...by emailing us at [email protected]. If you are looking for plasmids used in E. coli and yeast, you’re ...

...get to talk to scientists from around the world making breakthrough discoveries!” I knew right then I ...

...powered by 100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown...

...all ourselves. Therefore, in this blog post we’re taking a moment to reach out to you, our readers, and ...

... to use them in experiments. Best practices!). Making the find plasmids badge appear So how do you make...

...educational outreach. Many thanks to Alex Chavez for taking the time to sit down and talk with us.  Alejandro...

...download the CRISPR eBook today! P.S - If you’re looking for a quick refresher before you dive into the ...

...AAV PHP.eB Controls Deisseroth New serotype Looking for more viral vectors? Browse the full viral services...

... We’ve taken care of all the steps going from streaking to a single colony to DNA extraction and quantification...

...AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? Browse our in-stock viral...

...your viral packaging service in the same place, making it easier to go from selection to viral vector....