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We narrowed to 929 results for: KIN

Showing: 751 - 800 of 929 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations...

  2. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... used as a test to see how well treatments are working to fight the cancer.  Beyond early modifications...Asbestos Disease Awareness Organization (ADAO) are working toward such a ban, and the president of the organization...

  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...overly confluent or too old. Ensuring that you are working with healthy, happy cells is one of the easiest...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...of Replication The Promoter Region – Let's Go! Looking for More Plasmids 101 Articles?  Find them all ...

  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...biologist you would say. The student was of course talking about the now well-known FRET, aka Fluorescence...and the extinction coefficient of the acceptor (linking the quantity of absorbed light, at a given wavelength...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...

  9. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune, and/or inflammatory...

  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...thoughts on research statements. Snapp, who is always looking for ways to improve his eBook, adds: "If you have... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...

  12. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...optimal promoter for your gene of interest, painstakingly cloned into the perfect empty backbone, made...Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles?  Find them all ...

  13. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...gRNAs to choose from. With so many possibilities, picking a gRNA with an optimized sequence is of primary...detectable mutations (Veres et al., 2014). When working with single-cell clones, the authors note that ...

  14. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... MQ1.) The Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24...Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...cell types and are useful if you're going to be working in several different systems or want to transduce...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

  16. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii...

  19. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...mutations are essential data points for investigators looking to definitively link genotype and phenotype. It... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

  20. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...cytometry particularly well to complex experiments looking at a variety of different targets. If you are planning...43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current...

  21. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the smallest structures...microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic or static...

  22. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ... 98-99% target modification in primary T cells, making them a promising tool for cell therapy applications...

  23. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...efficiency is so high that some applications may permit working with edited “pools” of cells, eliminating the time-consuming...telomere length. Telomere trimming can be assayed by looking for the production of ECT-DNA (extra-chromosomal...

  24. New Coomassie Protocol Video

    Type
    Blog Post
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

  29. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...

  34. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...

  35. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... has a large international audience, including working with distributors across the world to help increase...

  37. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...keep track of reagents in your lab. With barcode tracking, you will always be able to locate materials quickly...

  43. Call for Guest Bloggers

    Type
    Blog Post
    ...all ourselves. Therefore, in this blog post we’re taking a moment to reach out to you, our readers, and ...

  46. CRISPR Cheat Sheet

    Type
    Blog Post
    ...download the CRISPR eBook today! P.S - If you’re looking for a quick refresher before you dive into the ...

  48. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? Browse our in-stock viral...

  50. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...Feng New viral service, multiple serotypes Looking for more viral vectors? Browse the full viral services...
Showing: 751 - 800 of 929 results