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We narrowed to 1,092 results for: addgene

Showing: 751 - 800 of 1092 results

  1. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...mVenus as a reference protein is available from Addgene. Another way to dodge cellular variation observed...based biosensors.     Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...2020 This post was contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist ...find validated gRNAs for your next experiment in Addgene's Validated gRNA Sequence Datatable. A more extended...://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen to our Podcast with John Doench Learn... the Broad Institute and has worked with many Addgenies to help improve the understanding, curation, and...Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR Basics with Our CRISPR...

  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR” version...popular target for labeling these cells. Check out Addgene’s Neuroscience Antibody Collection to find a recombinant...patterns from recombinant antibodies produced at Addgene (N206A/8R) were compared to the hybridoma-derived...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac... to help us expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase...PMCID: PMC3627465. Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase...Repair Is Used for Genome Editing Resources on Addgene.org Find Vectors with the piggyBac™ Transposon Catch...

  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use...CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage...CRISPR system in E. coli. The system available from Addgene consists of two plasmids: pCas9: carries Cas9 ...://doi.org/10.1093/nar/gkw625 Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ... out about CRISPR Multiplexing Resources on Addgene.org Browse our CRISPR Guide Pages Find Bacterial...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...s41586-021-03886-5 Additional resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for ...beyond spCas9 Cas proteins More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR...

  12. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...few of the newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR...PEDAR plasmids   Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... Various Addgenies...

  13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...s00018-019-03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...) Golden Gate assembly (backbones available at Addgene) with examples of potential synthetic chromatin...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... Various Addgenies...

  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...PubMed PMID: 20036813. Additional resources on the Addgene blog Read our cancer related blog posts Learn ...  Find plasmids for your research Learn about Addgene's viral service ...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

  16. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide AAV Viral Preps References  Chen...Resources and references More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements Viral...

  17. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858.   Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...

  18. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo Sabatini Wenbiao...Wickersham Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering...

  19. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...are publicly available, including those found at Addgene! Figure 1: Strategy for assembling the barcoded...PubMed Central PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA...about CRISPR/Optogentics Tools Resources on Addgene.org Catch up on Your CRISPR Background with Our ...

  20. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide... PMCID: PMC514534. Additional Resources on the Addgene Blog Read Our Lentivirus FAQ Learn How Lentivirus...

  21. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...

  22. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...different research applications they can be used for. Addgene’s Antibody Plasmid Collection includes plasmids ...10.3389/fcimb.2013.00072 Additional resources on the Addgene blog: Read about another reagent sharing platform...

  23. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...that can make or break a lab experience. Previous Addgene blogs have discussed the process that goes into...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career...science.caredit.abi6939 Additional resources on the Addgene blog Check out our resources for early career ...

  24. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...available through Addgene. This means you could perform a screen with one library from Addgene, and then use...PMCID: PMC5292764. Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design ...melanogaster, and T. gondii are available through www.addgene.org/crispr/libraries/. Additional libraries are...gRNA Tips for a 1st CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening...

  25. Important Considerations When Using AAVs

    Type
    Blog Post
    ...tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location, location, location! What do buying...populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors...PubMed PMID: 28073291. Additional Resources on the Addgene Blog AAV: A Versatile Viral Tool for Gene Expression..., AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/aav-guide. Where in the tissue...Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use...

  26. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...AAV plasmids containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice... other tissues.  Additional resources on the Addgene blog Read more about using AAV for retinal gene...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...

  27. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...an extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right...

  28. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP...References and resources:  Additional Resources on the Addgene blog CRISPR101: Editing the Epigenome  Mapping...the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read our CRISPR Guide to Epigenetics References...

  30. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ... using Nanoblades? Plasmids are available from Addgene. Genome editing primary cells with Nanoblades Primary...PMCID: PMC 6318322. Additional resources on the Addgene blog Learn about RNP delivery Learn about CRISPR...Browse our CRISPR 101 blog post Resources on Addgene.org Check out our comprehensive CRISPR guide Find...

  32. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...

  33. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning...PMCID: PMC3207894.  Additional Resources on the Addgene Blog Read other Plasmids 101 blog posts New to...Download the Plasmids 101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find...

  34. dTAG - You're it!

    Type
    Blog Post
    ...generated a series of plasmids that are available from Addgene. These plasmids enable lentiviral expression of...molecule: dTAG-47 Additional Resources on the Addgene Blog Easi-CRISPR: Generating Knock-In and Conditional...endogenous gene tagging in human cells Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for...

  35. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... use. Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD from Northeastern University...proteins in plant root exudates Resources on the Addgene Blog Learn about inducible promoters Learn about...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...

  37. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...

  38. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor Pages   Recent innovations have demonstrated...PubMed PMID: 26210398. Additional Resources on the Addgene Blog Tips for using FRET in Your Experiments Learn...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...

  39. 6 Tips for Grant Writing

    Type
    Blog Post
    ... why they should give you money. Check out the Addgene Blog for Some Ways to Improve Your Science Communication...multiplex assays.   Additional Resources on the Addgene Blog Learn How to Become a Grant Writing Professional...

  42. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ...Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management for Scientists...a 5-part blog on the topic:  http://blog.addgene.org/management-for-scientists-managing-vs-leading. The...

  45. Technical Design of a Western Blot

    Type
    Blog Post
    ...Antibodies Resources on Addgene.org Addgene's Western Blot Protocol Addgene's Western Blot Protocol Video...Looking for a western blot protocol? Check out Addgene's western blot protocol and protocol video!  Antibodies...Resources and references More resources on the Addgene blog Antibodies 101: The Basics of Western BlottingAntibodies... Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan Rego, and Amrita Rhodes...

  46. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...perform pha-1 co-conversion are available through Addgene. In 2019, Farboud et al. described another similar...To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives...about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design and...

  47. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

  48. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable practices for...relatively little work after the initial setup. (Addgene actually uses some of these — if you’re curious...Nyla Naim. Resources Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different...: Easy Action Items Additional resources on addgene.org DNA Quantification Protocol Recovering Plasmid...

  49. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...
Showing: 751 - 800 of 1092 results